Slc12a2 (NM_009194) Mouse Tagged ORF Clone

CAT#: MR227334

Slc12a2 (Myc-DDK-tagged) - Mouse solute carrier family 12, member 2 (Slc12a2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc12a2
• Synonyms: 9330166H04Rik; mBSC2; Nkcc1; sy-ns
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227334 representing NM_009194
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGGGCCCGCGGCGCCCTCGTCTGGCGCCCCGCGGCCCGCCCGGGATGGGGACGCGCCGCTGG
  CTGCCGCCGCCGGGGTGGACCTGCCCGGCACGGCCGTGCCCTCCGGCCAAGAGGATGCTACTACGGCTGG
  GAGGCAGGCTGGCGGCGGGGTGCGCGGAGAGGGAACCCCAGCGGCGGGCGATGGGCTGGGCAGACCTCTG
  GGGCCCACCCCCAGCCAGAGCCGCTTCCAGGTGGACCCGGTTTCCGAGAACGCCGGGCGGGCCGCGGCGG
  CTGCGGCGGCGGCGGCGGCGGCTGCGGCTGCGGCCGGGGCGGCGGGCAAGGAGACCCCCGCGGCCGGGAA
  AGCCGGCGGTGAGAGCGGCGTGGCCAAGGGCAGCGAGGAAGCCAAGGGCCGCTTCCGCGTGAACTTCGTG
  GACCCGGCTGCCTCGTCGTCGGCGGACGACAGCCTGTCGGATGCGGCGGGGGTCGGTGGCGACGGGCCCA
  ACGTGAGCTTCCAGAACGGCGGGGACACGGTGCTGAGCGAGGGCAGCAGCCTGCACTCGGGGGGTGGCAG
  TGGACACCACCAGCAGTACTACTATGACACCCACACCAACACCTACTACCTGCGCACCTTCGGCCACAAC
  ACCATGGACGCGGTGCCCAGGATCGACCACTACCGGCACACGGCCGCGCAGCTGGGCGAGAAGCTGCTCC
  GGCCCAGCCTGGCAGAGCTCCACGATGAGCTGGAAAAGGAACCTTTTGAGGATGGCTTTGCGAATGGAGA
  AGAAAGTACTCCAACCAGAGATGCTGTGGTCGCATACACTGCCGAGAGTAAAGGAGTTGTAAAGTTTGGC
  TGGATCAAGGGTGTATTAGTCCGCTGCATGTTAAACATTTGGGGTGTGATGCTCTTTATTAGATTGTCAT
  GGATCGTGGGTCAAGCTGGGATAGGTCTCTCTGTCGTCGTAATAGCAATGGCCACTGTTGTGACAACTAT
  CACAGGATTGTCTACTTCAGCAATAGCTACCAATGGGTTCGTGAGAGGAGGAGGAGCGTACTATTTAATC
  TCTAGAAGTCTAGGGCCGGAATTTGGTGGTGCAATTGGCTTGATCTTTGCTTTTGCCAATGCGGTGGCCG
  TTGCTATGTATGTTGTCGGATTCGCAGAGACTGTGGTGGAGCTGCTTAAGGAACATTCCATACTTATGAT
  AGATGAAATCAATGATATCCGAATTATTGGAGCCATTACAGTCGTGATTCTTCTGGGCATCTCAGTGGCT
  GGAATGGAGTGGGAAGCAAAGGCTCAGATTGTGCTGCTGGTGATCCTTCTCCTGGCCATCGCCGACTTCG
  TCATAGGGACGTTTATCTCACTGGAGAGCAAGAAGCCCAAGGGTTTCTTTGGCTATAAATCTGAAATATT
  TAATGAGAACTTTGGGCCGGATTTTCGAGAAGAAGAGACTTTCTTCTCTGTATTTGCCATCTTTTTTCCT
  GCTGCAACTGGTATTCTAGCTGGAGCAAATATCTCAGGTGATCTTGCAGATCCTCAGTCAGCCATACCCA
  AAGGAACACTCTTAGCCATTTTGATTACTACAGTGGTTTACATAGGAATTGCAGTGTCTGTGGGTTCGTG
  TGTTGTTCGGGATGCCACTGGCAATGTTAATGATACCATTACAACAGAGCTGACAAACTGTACTTCTGCA
  GCCTGCAAACTAAACTTTGACTTCTCTTATTGTGAGAGCAATACTTGTTCTTACGGGCTAATGAACAACT
  TTCAGGTGATGAGCATGGTGTCAGGATTTGCACCCTTGATCTCTGCAGGGATCTTTTCAGCCACGCTCTC
  GTCAGCACTAGCATCTCTTGTGAGTGCTCCCAAAATATTTCAGGCTCTATGTAAGGACAATATCTACCCA
  GCTTTCCAGATGTTTGCTAAAGGTTATGGGAAAAATAATGAACCGCTTCGTGGTTACATCTTAACATTTC
  TGATTGCACTGGGATTCATCCTGATTGCTGAATTGAACGTTATTGCTCCAATTATCTCTAATTTCTTCCT
  TGCATCGTATGCCTTAATCAACTTTTCAGTATTCCATGCATCACTTGCAAAATCTCCAGGATGGCGTCCT
  GCATTCAAATACTACAACATGTGGATCTCCCTTATTGGAGCAATTCTTTGTTGCATAGTGATGTTTGTCA
  TTAACTGGTGGGCTGCGTTGCTCACATATGTGATAGTCCTTGGGCTGTACATTTATGTTACTTACAAAAA
  ACCAGATGTGAACTGGGGATCCTCCACACAAGCGCTGACTTACCTCAGTGCACTGCAGCATTCAATCCGT
  CTTTCTGGGGTGGAGGACCATGTGAAAAACTTCAGACCACAGTGCCTTGTAATGACAGGTTCTCCAAACT
  CACGGCCTGCTTTACTTCATCTTGTTCATGACTTCACGAAAAATGTTGGTTTAATGATCTGTGGCCATGT
  GCATATGGGCCCCAGAAGACAAGCTATGAAAGAGATGTCCATTGATCAAGCCAGATATCAGCGATGGCTC
  ATTAAAAACAAAATGAAGGCTTTCTATGCTCCGGTTCACGCAGATGACTTGCGAGAAGGTGCACAATACT
  TGATGCAGGCTGCTGGACTTGGTCGTATGAAGCCAAACACACTTGTCCTGGGATTTAAGAAAGATTGGTT
  ACAAGCCGATATGAGGGATGTGGATATGTATATAAACTTATTTCATGATGCTTTTGACATACAATTTGGA
  GTTGTGGTTATCCGCCTGAAGGAAGGACTGGATATATCACACCTCCAAGGTCAGGAAGAATTATTATCTT
  CACAAGAAAAATCACCTGGTACCAAGGATGTGGTAGTAAATGTGGATTACAGTAAAAAGTCAGATCAAGA
  TACTTGCAAATCATCTGGTGAAAAATCCATTACACAGAAAGATGAGGAAGAGGATGGCAAGACTCCAACT
  CAGCCACTGTTGAAAAAAGAATCCAAAGGCCCTATTGTGCCTTTAAATGTAGCTGACCAAAAGCTTCTTG
  AAGCTAGCACACAGTTTCAGAAAAAACAAGGGAAGAATACTATAGATGTCTGGTGGCTTTTTGATGATGG
  AGGTTTGACATTGTTGATACCCTATCTTCTGACTACCAAGAAAAAGTGGAAAGATTGTAAGATCCGAGTA
  TTCATTGGTGGAAAAATAAACAGAATAGACCATGACCGGAGAGCGATGGCTACTTTATTGAGTAAATTCC
  GGATAGACTTCTCTGATATCATGGTCCTTGGAGATATCAACACCAAGCCAAAAAAAGAAAATATTATTGC
  TTTTGATGACATGATCGAGCCGTACAGACTTCACGAAGATGATAAAGAACAGGACATTGCAGATAAGATG
  AAAGAAGATGAGCCTTGGCGAATCACAGATAATGAGCTTGAGCTTTATAAGACTAAGACATATCGACAGA
  TCAGGTTAAATGAATTATTAAAGGAGCATTCAAGCACAGCTAACATTATTGTCATGAGTCTTCCAGTCGC
  ACGGAAAGGCGCTGTGTCCAGTGCTCTGTATATGGCTTGGTTAGAAGCTCTCTCGAAGGACCTGCCACCA
  GTCCTCCTGGTTCGTGGGAATCATCAGAGCGTCCTTACCTTCTACTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227334 representing NM_009194
  Red=Cloning site Green=Tags(s)
  
  MEPGPAAPSSGAPRPARDGDAPLAAAAGVDLPGTAVPSGQEDATTAGRQAGGGVRGEGTPAAGDGLGRPL
  GPTPSQSRFQVDPVSENAGRAAAAAAAAAAAAAAAGAAGKETPAAGKAGGESGVAKGSEEAKGRFRVNFV
  DPAASSSADDSLSDAAGVGGDGPNVSFQNGGDTVLSEGSSLHSGGGSGHHQQYYYDTHTNTYYLRTFGHN
  TMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVAYTAESKGVVKFG
  WIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVVVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLI
  SRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVA
  GMEWEAKAQIVLLVILLLAIADFVIGTFISLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFP
  AATGILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTITTELTNCTSA
  ACKLNFDFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYP
  AFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRP
  AFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIR
  LSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQARYQRWL
  IKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFG
  VVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVNVDYSKKSDQDTCKSSGEKSITQKDEEEDGKTPT
  QPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRV
  FIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIEPYRLHEDDKEQDIADKM
  KEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPP
  VLLVRGNHQSVLTFYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009194
• ORF Size: 3618 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009194.1, NM_009194.2, NM_009194.3, NP_033220.2
• RefSeq Size: 6520
• RefSeq ORF: 3621
• Locus ID: 20496
• MW: 131.1 kDa
• Gene Summary: Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. [UniProtKB/Swiss-Prot Function]