Mmp13 (NM_008607) Mouse Tagged ORF Clone

CAT#: MR227427

Mmp13 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 13 (Mmp13)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mmp13
• Synonyms: Clg; MMP-13; Mmp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227427 representing NM_008607
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATTCAGCTATCCTGGCCACCTTCTTCTTGTTGAGCTGGACTCCCTGTTGGTCCCTGCCCCTTCCCT
  ATGGTGATGATGATGATGATGACCTGTCTGAGGAAGACCTTGTGTTTGCAGAGCACTACTTGAAATCATA
  CTACCATCCTGCGACTCTTGCGGGAATCCTGAAGAAGTCTACAGTGACCTCCACAGTTGACAGGCTCCGA
  GAAATGCAATCTTTCTTTGGCTTAGAGGTGACTGGCAAACTTGATGATCCCACCTTAGACATCATGAGAA
  AACCAAGATGTGGAGTGCCTGATGTGGGTGAATACAATGTTTTCCCTAGAACACTCAAATGGTCCCAAAC
  GAACTTAACTTACAGGATTGTGAACTATACTCCTGATATGTCCCATTCTGAAGTGGAGAAGGCCTTCAGA
  AAAGCCTTCAAGGTCTGGTCTGATGTGACACCACTGAATTTCACCAGAATCTATGATGGCACTGCTGACA
  TCATGATATCTTTTGGGACTAAAGAACATGGTGACTTCTACCCATTTGATGGACCTTCTGGTCTTCTGGC
  ACACGCTTTTCCTCCTGGACCAAACTATGGTGGGGATGCCCATTTTGATGATGATGAAACCTGGACAAGC
  AGTTCCAAAGGCTACAACTTGTTTATTGTTGCTGCCCATGAGCTTGGCCACTCCCTAGGTCTGGATCACT
  CCAAGGACCCAGGAGCCCTGATGTTTCCCATCTATACCTACACTGGCAAAAGCCATTTCATGCTTCCTGA
  TGATGACGTTCAAGGAATTCAGTTTCTTTATGGTCCAGGCGATGAAGACCCCAACCCTAAGCATCCCAAA
  ACACCAGAGAAGTGTGACCCAGCCCTATCCCTTGATGCCATTACCAGTCTCCGAGGAGAAACTATGATCT
  TTAAAGACAGATTCTTCTGGCGCCTGCACCCTCAGCAGGTTGAGGCTGAGCTCTTTTTGACAAAGTCCTT
  TTGGCCAGAACTTCCCAACCATGTGGATGCTGCATATGAACATCCATCCCGTGACCTTATGTTTATCTTT
  AGAGGGAGAAAATTCTGGGCTCTGAATGGTTATGACATTCTGGAAGGTTATCCCAGAAAAATATCTGACC
  TGGGATTCCCAAAAGAGGTGAAGAGACTGAGCGCTGCGGTTCACTTTGAGAACACGGGGAAGACCCTCTT
  CTTCTCTGAGAACCACGTGTGGAGTTATGATGATGTTAACCAGACTATGGACAAAGATTATCCCCGCCTC
  ATAGAAGAGGAATTCCCTGGAATTGGCAACAAAGTAGATGCTGTCTATGAGAAAAATGGCTATATCTACT
  TTTTCAATGGGCCCATACAGTTTGAATACAGTATCTGGAGTAATCGCATTGTGAGAGTCATGCCAACAAA
  TTCCATATTGTGGTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227427 representing NM_008607
  Red=Cloning site Green=Tags(s)
  
  MHSAILATFFLLSWTPCWSLPLPYGDDDDDDLSEEDLVFAEHYLKSYYHPATLAGILKKSTVTSTVDRLR
  EMQSFFGLEVTGKLDDPTLDIMRKPRCGVPDVGEYNVFPRTLKWSQTNLTYRIVNYTPDMSHSEVEKAFR
  KAFKVWSDVTPLNFTRIYDGTADIMISFGTKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDDDETWTS
  SSKGYNLFIVAAHELGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQFLYGPGDEDPNPKHPK
  TPEKCDPALSLDAITSLRGETMIFKDRFFWRLHPQQVEAELFLTKSFWPELPNHVDAAYEHPSRDLMFIF
  RGRKFWALNGYDILEGYPRKISDLGFPKEVKRLSAAVHFENTGKTLFFSENHVWSYDDVNQTMDKDYPRL
  IEEEFPGIGNKVDAVYEKNGYIYFFNGPIQFEYSIWSNRIVRVMPTNSILWC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008607
• ORF Size: 1416 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008607.1, NM_008607.2, NP_032633.1
• RefSeq Size: 2675
• RefSeq ORF: 1419
• Locus ID: 17386
• MW: 54.6 kDa
• Gene Summary: This gene encodes a member of the matrix metalloproteinase family that plays a role in wound healing, skeletal development and bone remodeling. The encoded protein is activated by the removal of an N-terminal activation peptide to generate a zinc-dependent endopeptidase enzyme that can cleave various native collagens, including types I - IV, X and XIV. Mice lacking the encoded protein display profound defects in growth plate cartilage as well as a delay in the endochondral bone development. Lack of the encoded protein also impairs the wound healing process due to reduced keratinocyte migration and vascular density at the wound site. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. [provided by RefSeq, Jun 2015]