Enpp1 (NM_008813) Mouse Tagged ORF Clone

CAT#: MR227498

Enpp1 (Myc-DDK-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Enpp1
• Synonyms: 4833416E15Rik; AI428932; C76301; CD203c; E-NPP 1; E-NPP1; Ly-41; M6S1; NPP1; Npps; PC-1; Pca; Pca-1; Pdnp1; ttw; twy
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227498 representing NM_008813
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGCGACGGCGACCAGGCAGGGCACGGGCCCCGGCACGGGTCAGCGGGAAACGGTCGCGAGCTCG
  AGTCACCAGCCGCGGCTTCGCTGCTCGCGCCCATGGACCTAGGAGAGGAGCCGCTGGAGAAGGCGGAGCG
  TGCCCGCCCTGCCAAAGACCCCAACACCTACAAAGTGCTGTCGCTGGTTTTGTCAGTATGTGTGCTAACA
  ACAATTCTTGGTTGTATATTTGGGTTGAAACCAAGCTGCGCCAAAGAAGTAAAAAGTTGCAAAGGCCGCT
  GCTTTGAAAGGACGTTCAGCAACTGTCGCTGTGATGCTGCCTGTGTCAGCCTTGGGAACTGCTGTCTGGA
  TTTCCAGGAGACCTGTGTGGAACCAACACATATATGGACTTGCAACAAATTCAGGTGCGGTGAGAAGAGG
  CTGTCCAGGTTCGTGTGCTCCTGTGCAGACGACTGCAAAACCCACAATGACTGTTGCATCAACTACAGTT
  CTGTGTGCCAAGATAAGAAGAGTTGGGTAGAAGAAACATGTGAAAGCATCGATACCCCGGAGTGTCCAGC
  AGAGTTTGAATCACCCCCTACTCTCTTGTTTTCTTTGGATGGATTCAGAGCTGAGTATTTGCACACCTGG
  GGTGGACTTCTTCCTGTCATTAGCAAGCTGAAAAACTGTGGAACATACACTAAAAACATGAGGCCTATGT
  ACCCTACCAAGACGTTTCCCAATCATTACAGCATCGTCACAGGACTTTATCCGGAATCCCATGGCATAAT
  TGATAACAAGATGTATGATCCCAAAATGAATGCTTCTTTCTCGCTTAAAAGTAAAGAGAAATTCAACCCT
  TTGTGGTACAAAGGACAGCCGATTTGGGTGACCGCTAATCATCAGGAGGTCAAGTCCGGCACATACTTTT
  GGCCAGGATCAGATGTGGAGATTGACGGGATTCTACCAGATATCTACAAAGTGTATAATGGGTCAGTACC
  ATTTGAAGAAAGGATTTTAGCTGTTCTTGAGTGGCTGCAGCTTCCTAGCCATGAAAGACCACACTTTTAC
  ACTCTGTATTTAGAAGAACCAGATTCTTCAGGGCATTCACATGGACCAGTCAGCAGCGAGGTCATCAAGG
  CCTTGCAGAAGGTTGACCGCCTGGTCGGCATGCTGATGGACGGCCTGAAGGACCTGGGCTTGGATAAATG
  CCTGAACCTCATCCTCATTTCAGATCACGGCATGGAACAAGGCAGCTGTAAGAAGTATGTGTACCTGAAT
  AAGTACTTGGGGGATGTGAACAATGTGAAAGTTGTGTATGGACCTGCTGCTCGGTTGAGACCCACTGATG
  TTCCAGAGACATACTATTCATTTAACTATGAAGCCCTTGCAAAAAATCTTTCTTGCCGGGAGCCAAACCA
  GCATTTCCGGCCTTACCTGAAACCCTTCTTACCCAAGCGCTTACACTTCGCTAAAAGTGACCGGATTGAG
  CCCCTGACCTTCTACCTGGACCCTCAGTGGCAACTTGCGTTGAATCCATCAGAGAGGAAATATTGTGGAA
  GTGGATTTCATGGCTCTGACAACTTGTTTTCAAACATGCAAGCTCTTTTTATTGGCTATGGACCTGCCTT
  CAAGCATGGTGCTGAAGTTGACTCCTTTGAAAATATTGAAGTCTATAACTTAATGTGTGACTTACTGGGT
  TTGATCCCAGCTCCCAATAATGGAAGTCATGGCAGCCTTAACCACCTTCTAAAGAAACCCATTTACAACC
  CAAGTCATCCCAAAGAAGAGGGCTTCCTGTCTCAGTGTCCAATCAAATCAACATCCAATGACCTCGGCTG
  CACATGTGACCCTTGGATTGTGCCAATCAAGGACTTTGAGAAACAGCTTAATCTGACCACAGAAGATGAT
  GACATTTACCATATGACTGTACCCTACGGGAGGCCCCGGATTCTACTGAAGCAGCACCGCGTCTGTCTAC
  TCCAGCAGCAACAGTTCTTGACCGGATACAGCCTGGACCTCCTCATGCCCCTCTGGGCGTCTTACACCTT
  CCTCAGTAATGACCAATTCTCCAGAGATGACTTTTCCAACTGTCTGTACCAGGATCTTCGGATTCCCCTT
  AGTCCTGTGCATAAATGTTCCTATTACAAAAGCAATTCTAAGCTAAGTTATGGGTTCCTCACGCCACCGA
  GACTAAATAGAGTTTCAAATCACATATACTCTGAAGCGCTGCTTACATCTAATATAGTGCCAATGTATCA
  GAGTTTTCAAGTTATATGGCACTACCTTCATGACACCCTACTGCAAAGGTATGCCCATGAAAGGAACGGC
  ATCAATGTTGTCAGCGGTCCCGTGTTTGACTTTGATTATGACGGACGCTATGATTCCTTAGAGATCCTGA
  AACAAAACAGCAGAGTCATCCGAAGCCAGGAAATTCTGATTCCGACTCACTTCTTCATTGTGCTCACCAG
  CTGCAAGCAGCTGTCTGAGACTCCCTTGGAGTGCTCCGCCTTAGAGTCCTCAGCTTACATACTGCCTCAT
  AGGCCTGATAACATTGAGAGCTGTACGCATGGGAAGCGGGAGTCTTCATGGGTCGAGGAGTTGCTGACCT
  TGCACAGAGCTCGGGTCACAGACGTGGAACTCATCACTGGCCTCAGCTTCTACCAGGACCGACAAGAGTC
  AGTTTCAGAACTGCTGAGGTTGAAAACACATTTGCCAATCTTCAGCCAAGAAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227498 representing NM_008813
  Red=Cloning site Green=Tags(s)
  
  MERDGDQAGHGPRHGSAGNGRELESPAAASLLAPMDLGEEPLEKAERARPAKDPNTYKVLSLVLSVCVLT
  TILGCIFGLKPSCAKEVKSCKGRCFERTFSNCRCDAACVSLGNCCLDFQETCVEPTHIWTCNKFRCGEKR
  LSRFVCSCADDCKTHNDCCINYSSVCQDKKSWVEETCESIDTPECPAEFESPPTLLFSLDGFRAEYLHTW
  GGLLPVISKLKNCGTYTKNMRPMYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNP
  LWYKGQPIWVTANHQEVKSGTYFWPGSDVEIDGILPDIYKVYNGSVPFEERILAVLEWLQLPSHERPHFY
  TLYLEEPDSSGHSHGPVSSEVIKALQKVDRLVGMLMDGLKDLGLDKCLNLILISDHGMEQGSCKKYVYLN
  KYLGDVNNVKVVYGPAARLRPTDVPETYYSFNYEALAKNLSCREPNQHFRPYLKPFLPKRLHFAKSDRIE
  PLTFYLDPQWQLALNPSERKYCGSGFHGSDNLFSNMQALFIGYGPAFKHGAEVDSFENIEVYNLMCDLLG
  LIPAPNNGSHGSLNHLLKKPIYNPSHPKEEGFLSQCPIKSTSNDLGCTCDPWIVPIKDFEKQLNLTTEDD
  DIYHMTVPYGRPRILLKQHRVCLLQQQQFLTGYSLDLLMPLWASYTFLSNDQFSRDDFSNCLYQDLRIPL
  SPVHKCSYYKSNSKLSYGFLTPPRLNRVSNHIYSEALLTSNIVPMYQSFQVIWHYLHDTLLQRYAHERNG
  INVVSGPVFDFDYDGRYDSLEILKQNSRVIRSQEILIPTHFFIVLTSCKQLSETPLECSALESSAYILPH
  RPDNIESCTHGKRESSWVEELLTLHRARVTDVELITGLSFYQDRQESVSELLRLKTHLPIFSQED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008813
• ORF Size: 2715 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008813.2, NM_008813.3, NM_008813.4, NP_032839.3
• RefSeq Size: 3224
• RefSeq ORF: 2718
• Locus ID: 18605
• MW: 103.5 kDa
• Gene Summary: This gene encodes a member of the nucleoside pyrophosphatase/phosphodiesterase family of enzymes that catalyzes the hydrolysis of pyrophosphate and phosphodiester bonds in nucleotide triphosphates and oligonucleotides, respectively, to generate nucleoside 5'-monophosphates. The encoded protein is a type II transmembrane glycoprotein that negatively regulates bone mineralization. Mice harboring a nonsense mutation in this gene, termed tiptoe walking (ttw), exhibit ectopic ossification of the spinal ligaments. The encoded protein binds to the insulin receptor, inhibits downstream signaling events and induces insulin resistance and glucose tolerance. This gene is located adjacent to a paralog on chromosome 10. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]