Mmp19 (NM_001164197) Mouse Tagged ORF Clone

CAT#: MR227676

Mmp19 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 19 (Mmp19), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mmp19
• Synonyms: MGC123370
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227676 representing NM_001164197
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTGGCAGCAGCTGTGGCTGGCATTCTTACTTCCCATGACAGTCTCAGGCCGGGCTCTGGGGCCTA
  CAGAGAAGGAGGCAGTCTTGGATTACCTGTTGCAGTATGGGTATCTACAGAAACCTCTAGAAGGAGCTGA
  TGACTTCAGGCTGGAAGATATCACAGAGGCTTTAAGAACTTTCCAGGAAGCATCTGGACTGCCCATTTCC
  GGTCAGATGGATGACGCCACAAGGGCCCGTATGAAGCAGCCCCGTTGTGGTCTGGAGGATCCCTTCAACC
  AGAAATCTCTTAAATACCTGCTTCTGGGCCACTGGAGAAAGAAGAACTTGACATTCCGCATCTTCAATGT
  GCCCTCCACCCTCTCACTTCCCAGAGTCCGAGCAGCCCTGCATCAAGCCTTTAAGTACTGGAGCAGCGTG
  GCTCCTCTGACCTTCCGGGAGGTGAAAGCTGGTTGGGCTGACATTCGCCTCTCTTTCCATGGCCGCCAAA
  GCCTGTACTGCTCCAATACCTTTGATGGGCCTGGAAAGGTCCTGGCCCATGCTGACATCCCAGAACTTGG
  GAGTATACACTTTGATAAAGATGAACTCTGGACTGAGGGGACCTATCAAGGAGTGAACCTGCGCATCATT
  GCGGCCCATGAAGTGGGCCATGCCCTAGGACTTGGGCACTCCCGATATACCCAGGCACTCATGGCTCCTG
  TCTATGCTGGCTACCAGCCCTTCTTCAAGCTGCATCCAGATGATGTGGCAGGGATCCAGGCTCTCTATGG
  CAAGAGGAGCCCAGAGACAAGAGATGAGGAGGAAGAGACCGAGATGCTCACTGTGTCTCCAGTGACTGCA
  AAACCTGGTCCCATGCCAAACCCCTGCAGCGGTGAGGTGGACGCCATGGTGCTGGGGCCTCGTGGGAAGA
  CTTATGCTTTCAAGGGCGACTATGTGTGGACTGTAACAGATTCAGGGCCAGGCCCCTTGTTCCAAATATC
  TGCCCTTTGGGAGGGGCTTCCTGGAAACCTAGATGCTGCCGTTTACTCTCCCCGGACACGACGGACTCAT
  TTCTTCAAGGGAAACAAGGTGTGGCGGTATGTGGATTTCAAGATGTCTCCTGGCTTTCCCATGAAATTCA
  ACAGAGTAGAGCCCAACCTGGATGCAGCTCTCTACTGGCCTGTTAATCAAAAGGTGTTCCTGTTTAAGGG
  CTCAGGATACTGGCAATGGGATGAACTGGCCAGAACTGACCTTAGCCGCTACCCTAAACCAATCAAGGAA
  CTGTTTACTGGAGTGCCAGACCGACCCTCGGCAGCTATGAGCTGGCAAGATGGCCAAGTCTACTTTTTCA
  AGGGCAAAGAGTATTGGCGCCTTAACCAGCAACTTCGAGTGGCAAAGGGCTATCCCAGAAATACGACACA
  CTGGATGCACTGTGGTTCTCAGACTCCAGACACTAACTCATCAACCGGGGACGTTACTCCTTCAACCACA
  GACACAGTCTTGGGTACCACTCCATCAACCATGGGCTCAACCTTGGACATTCCCTCAGCTACAGACTCTG
  CCTCCCTCTCATTCTCTGCTAATGTCACCCTGCTAGGGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227676 representing NM_001164197
  Red=Cloning site Green=Tags(s)
  
  MDWQQLWLAFLLPMTVSGRALGPTEKEAVLDYLLQYGYLQKPLEGADDFRLEDITEALRTFQEASGLPIS
  GQMDDATRARMKQPRCGLEDPFNQKSLKYLLLGHWRKKNLTFRIFNVPSTLSLPRVRAALHQAFKYWSSV
  APLTFREVKAGWADIRLSFHGRQSLYCSNTFDGPGKVLAHADIPELGSIHFDKDELWTEGTYQGVNLRII
  AAHEVGHALGLGHSRYTQALMAPVYAGYQPFFKLHPDDVAGIQALYGKRSPETRDEEEETEMLTVSPVTA
  KPGPMPNPCSGEVDAMVLGPRGKTYAFKGDYVWTVTDSGPGPLFQISALWEGLPGNLDAAVYSPRTRRTH
  FFKGNKVWRYVDFKMSPGFPMKFNRVEPNLDAALYWPVNQKVFLFKGSGYWQWDELARTDLSRYPKPIKE
  LFTGVPDRPSAAMSWQDGQVYFFKGKEYWRLNQQLRVAKGYPRNTTHWMHCGSQTPDTNSSTGDVTPSTT
  DTVLGTTPSTMGSTLDIPSATDSASLSFSANVTLLGA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164197
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq Size: 3275
• RefSeq ORF: 1368
• Locus ID: 58223
• MW: 51.4 kDa
• Gene Summary: This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme. Mice lacking the encoded protein develop a diet-induced obesity due to adipocyte hypertophy, exhibit decreased susceptibility to chemical carcinogen-induced skin tumors and early onset of tumoral angiogenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]