Etfbkmt (NM_001252095) Mouse Tagged ORF Clone

CAT#: MR228811

Mettl20 (myc-DDK-tagged) - Mouse methyltransferase like 20 (Mettl20), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Etfbkmt
• Synonyms: 4833442J19Rik; AI256744; ETFB-KMT; Mettl20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228811 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTCAGCCTGTGTTGGAAAGCTCCCAGGAGCCCATGGAGTTTCCTGCAGGCTGTGAACAATGGGT
  CTCCCCTGTTTCTCTGGAGGACAGTGGGGAGCTGTTTGGATCCTAAGATGAAAGCCTACCTGGAAGAGAA
  CACTGAAGTCACCAGCAGTGGTAGCCTTACCCCGGAAATCCAGTTACGGCTTTTAACCCCCAGGTGCAAG
  TTTTGGTGGGAAAGGGCTGACCTGTGGCCCTACAGTGATCCCTACTGGGCTATTTACTGGCCAGGAGGCC
  AGGCTCTGTCTAGGTATCTTTTGGATAACCCTGCTGTTGTTAGAGGAAAGTCTGTCTTAGATCTTGGGAG
  TGGATGTGGAGCTACAGCCATTGCTGCCAAGATGAGTGGAGCATCAAAGATCTTGGCCAACGACATAGAC
  CCCATTGCAGGAATGGCGATTACACTAAATTGCAAATTAAATGGACTGAATCCTTTTCCTGTTTTAACTA
  AAAACATTTTGAATACCCAACAAGGTAAGTTTGATCTCATTGTTCTTGGAGATATGTTTTATGATGAAGA
  CCTTGCAGACAGTCTTCATCTGTGGCTGCAGAACTACTTTTGGACCCATGGAACCCGAGTACTGATTGGT
  GACCCTGGGAGGCCCCAGTTCAGTGGACATAGCATTCGGCATCAACTGTACCAACTAGTAGAATACACAC
  TTCCAGAGCCAACTCAGCAAGAGAACAATGGACTGACCACAAGTGCTGTATGGGATTTTCATCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228811 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAFSLCWKAPRSPWSFLQAVNNGSPLFLWRTVGSCLDPKMKAYLEENTEVTSSGSLTPEIQLRLLTPRCK
  FWWERADLWPYSDPYWAIYWPGGQALSRYLLDNPAVVRGKSVLDLGSGCGATAIAAKMSGASKILANDID
  PIAGMAITLNCKLNGLNPFPVLTKNILNTQQGKFDLIVLGDMFYDEDLADSLHLWLQNYFWTHGTRVLIG
  DPGRPQFSGHSIRHQLYQLVEYTLPEPTQQENNGLTTSAVWDFHP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252095
• ORF Size: 768 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252095.1, NP_001239024.1
• RefSeq Size: 1688 bp
• RefSeq ORF: 768 bp
• Locus ID: 320204
• MW: 28.6 kDa