Nanog (NM_001289831) Mouse Tagged ORF Clone

CAT#: MR228969

Nanog (myc-DDK-tagged) - Mouse Nanog homeobox (Nanog), transcript variant 4

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nanog
• Synonyms: 2410002E02Rik; ecat4; ENK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228969 representing NM_001289831
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGCAGTTTTTCATCCCGAGAACTATTCTTGCTTACAAGGGTCTGCTACTGAGATGCTCTGCACAG
  AGGCTGCCTCTCCTCGCCCTTCCTCTGAAGACCTGCCTCTTCAAGGCAGCCCTGATTCTTCTACCAGTCC
  CAAACAAAAGCTCTCAAGTCCTGAGGCTGACAAGGGCCCTGAGGAGGAGGAGAACAAGGTCCTTGCCAGG
  AAGCAGAAGATGCGGACTGTGTTCTCTCAGGCCCAGCTGTGTGCACTCAAGGACAGGTTTCAGAAGCAGA
  AGTACCTCAGCCTCCAGCAGATGCAAGAACTCTCCTCCATTCTGAACCTGAGCTATAAGCAGGTTAAGAC
  CTGGTTTCAAAACCAAAGGATGAAGTGCAAGCGGTGGCAGAAAAACCAGTGGTTGAAGACTAGCAATGGT
  CTGATTCAGAAGGGCTCAGCACCAGTGGAGTATCCCAGCATCCATTGCAGCTATCCCCAGGGCTATCTGG
  TGAACGCATCTGGAAGCCTTTCCATGTGGGGCAGCCAGACTTGGACCAACCCAACTTGGAGCAGCCAGAC
  CTGGACCAACCCAACTTGGAACAACCAGACCTGGACCAACCCAACTTGGAGCAGCCAGGCCTGGACCGCT
  CAGTCCTGGAACGGCCAGCCTTGGAATGCTGCTCCGCTCCATAACTTCGGGGAGGACTTTCTGCAGCCTT
  ACGTACAGTTGCAGCAAAACTTCTCTGCCAGTGATTTGGAGGTGAATTTGGAAGCCACTAGGGAAAGCCA
  TGCGCATTTTAGCACCCCACAAGCCTTGGAATTATTCCTGAACTACTCTGTGACTCCACCAGGTGAAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228969 representing NM_001289831
  Red=Cloning site Green=Tags(s)
  
  MPAVFHPENYSCLQGSATEMLCTEAASPRPSSEDLPLQGSPDSSTSPKQKLSSPEADKGPEEEENKVLAR
  KQKMRTVFSQAQLCALKDRFQKQKYLSLQQMQELSSILNLSYKQVKTWFQNQRMKCKRWQKNQWLKTSNG
  LIQKGSAPVEYPSIHCSYPQGYLVNASGSLSMWGSQTWTNPTWSSQTWTNPTWNNQTWTNPTWSSQAWTA
  QSWNGQPWNAAPLHNFGEDFLQPYVQLQQNFSASDLEVNLEATRESHAHFSTPQALELFLNYSVTPPGEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289831
• ORF Size: 840 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001289831.1, NP_001276760.1
• RefSeq Size: 2004
• RefSeq ORF: 843
• Locus ID: 71950
• MW: 32.3 kDa
• Gene Summary: The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]