Gjb1 (NM_001302496) Mouse Tagged ORF Clone

CAT#: MR228988

Gjb1 (myc-DDK-tagged) - Mouse gap junction protein, beta 1 (Gjb1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gjb1
• Synonyms: Cnx32; connexin-32; Cx32
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228988 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTGGACAGGTCTATACACCTTGCTCAGTGGCGTGAATCGGCACTCTACAGCCATTGGCCGAGTAT
  GGCTGTCTGTCATCTTCATCTTCAGAATCATGGTGCTGGTGGTGGCTGCTGAGAGCGTGTGGGGGGATGA
  GAAGTCCTCTTTCATCTGTAACACCCTCCAGCCGGGCTGCAACAGCGTCTGCTATGACCATTTTTTCCCC
  ATCTCCCACGTGCGCCTATGGTCCCTGCAGCTTATCTTGGTTTCCACCCCAGCTCTCCTCGTGGCAATGC
  ACGTAGCTCACCAACAGCACATAGAAAAGAAAATGCTACGGCTTGAGGGGCATGGGGACCCCCTTCACCT
  GGAAGAGGTAAAGAGACACAAGGTGCACATCTCAGGGACACTGTGGTGGACCTATGTCATCAGTGTGGTG
  TTCCGGCTGCTGTTCGAGGCTGTCTTCATGTATGTCTTCTATCTGCTCTACCCCGGCTATGCCATGGTGC
  GGCTGGTCAAGTGTGAAGCCTTCCCCTGCCCCAACACAGTGGACTGCTTCGTGTCCCGCCCCACCGAGAA
  AACCGTCTTCACTGTCTTTATGCTCGCAGCCTCCGGCATCTGCATTATCCTCAACGTGGCGGAGGTGGTG
  TACCTCATCATCCGGGCCTGTGCCCGCCGTGCTCAGCGCCGCTCCAATCCGCCCTCCCGCAAGGGCTCGG
  GCTTCGGCCACCGCCTCTCACCTGAATACAAGCAGAATGAGATCAACAAGCTGCTGAGCGAGCAGGATGG
  CTCTCTGAAAGACATACTGCGCCGCAGCCCTGGCACAGGGGCCGGGCTCGCTGAAAAGAGCGACCGATGC
  TCAGCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228988 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNWTGLYTLLSGVNRHSTAIGRVWLSVIFIFRIMVLVVAAESVWGDEKSSFICNTLQPGCNSVCYDHFFP
  ISHVRLWSLQLILVSTPALLVAMHVAHQQHIEKKMLRLEGHGDPLHLEEVKRHKVHISGTLWWTYVISVV
  FRLLFEAVFMYVFYLLYPGYAMVRLVKCEAFPCPNTVDCFVSRPTEKTVFTVFMLAASGICIILNVAEVV
  YLIIRACARRAQRRSNPPSRKGSGFGHRLSPEYKQNEINKLLSEQDGSLKDILRRSPGTGAGLAEKSDRC
  SAC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001302496
• ORF Size: 852 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001302496.1, NP_001289425.1
• RefSeq Size: 1525
• RefSeq ORF: 852
• Locus ID: 14618
• MW: 32 kDa
• Gene Summary: This gene is a member of the gap junction protein (connexin) family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of ions and small molecules between cells. Mutations in a similar gene in human cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Oct 2014]