Tpm3 (NM_001293748) Mouse Tagged ORF Clone

CAT#: MR229004

Tpm3 (myc-DDK-tagged) - Mouse tropomyosin 3, gamma (Tpm3), transcript variant Tpm3.12

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tpm3
• Synonyms: gamma-TM; hTM30nm; hTMnm; Tm5NM; TM30nm; TMnm; Tpm-5; Tpm5; Trop-5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229004 representing NM_001293748
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAGGCCATCAAGAAAAAGATGCAGATGCTGAAGTTAGACAAAGAGAATGTTCTGGACCGAGCTG
  AGCAAGCTGAAGCTGAGCAGAAACAGGCAGAAGAAAGAAGCAAGCAGCTAGAGGATGAACTAGCAACCAT
  GCAGAAGAAGCTGAAAGGGACAGAGGATGAGCTGGACAAGTATTCGGAAGCTTTAAAGGATGCTCAGGAG
  AAGCTGGAGCTAGCAGAGAAGAAGGCAGCCGACGCTGAAGCTGAGGTGGCCTCCTTGAACCGCAGGATCC
  AGCTGGTTGAAGAGGAGCTGGACCGTGCGCAGGAGCGCCTTGCCACTGCTTTGCAGAAGCTGGAGGAAGC
  AGAGAAGGCTGCTGATGAGAGTGAGAGAGGTATGAAGGTGATTGAAAATCGGGCTCTAAAAGATGAAGAA
  AAGATGGAACTCCAGGAAATCCAGCTAAAGGAAGCAAAGCACATTGCAGAAGAGGCCGATAGGAAGTATG
  AAGAGGTGGCTCGTAAGTTGGTGATTATTGAAGGAGACTTGGAACGCACGGAGGAACGTGCTGAGCTGGC
  AGAGTCTAAGTGTTCTGAGCTGGAGGAGGAGCTGAAGAATGTCACCAACAACCTCAAGTCTCTTGAGGCT
  CAGGCGGAGAAGTACTCTCAAAAAGAAGACAAGTATGAAGAAGAAATAAAGATTCTTACTGATAAACTCA
  AGGAGGCAGAGACCCGTGCTGAGTTTGCTGAAAGATCGGTAGCCAAGCTGGAGAAGACCATTGATGACCT
  GGAAGACGAGCTCTATGCCCAGAAACTGAAGTACAAGGCCATTAGCGACGAGCTGGACCACGCCCTCAAT
  GACATGACCTCTATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229004 representing NM_001293748
  Red=Cloning site Green=Tags(s)
  
  MMEAIKKKMQMLKLDKENVLDRAEQAEAEQKQAEERSKQLEDELATMQKKLKGTEDELDKYSEALKDAQE
  KLELAEKKAADAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEE
  KMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESKCSELEEELKNVTNNLKSLEA
  QAEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEDELYAQKLKYKAISDELDHALN
  DMTSI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293748
• ORF Size: 855 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001293748.1, NP_001280677.2
• RefSeq Size: 1137
• RefSeq ORF: 858
• Locus ID: 59069
• MW: 33.4 kDa
• Gene Summary: Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. [UniProtKB/Swiss-Prot Function]