Hsd11b1 (NM_001044751) Mouse Tagged ORF Clone

CAT#: MR229057

Hsd11b1 (myc-DDK-tagged) - Mouse hydroxysteroid 11-beta dehydrogenase 1 (Hsd11b1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hsd11b1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229057 representing NM_001044751
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTTATGAAAAATTACCTCCTCCCGATCCTGGTGCTCTTCCTGGCCTACTACTACTATTCTACAA
  ATGAAGAGTTCAGACCAGAAATGCTCCAGGGAAAGAAAGTGATTGTCACTGGGGCCAGCAAAGGGATTGG
  AAGAGAAATGGCATATCATCTGTCAAAAATGGGAGCCCATGTGGTATTGACTGCCAGGTCGGAGGAAGGT
  CTCCAGAAGGTAGTGTCTCGCTGCCTTGAACTCGGAGCAGCCTCTGCTCACTACATTGCTGGCACTATGG
  AAGACATGACATTTGCGGAGCAATTTATTGTCAAGGCGGGAAAGCTCATGGGCGGACTGGACATGCTTAT
  TCTAAACCACATCACTCAGACCTCGCTGTCTCTCTTCCATGACGACATCCACTCTGTGCGAAGAGTCATG
  GAGGTCAACTTCCTCAGCTACGTGGTCATGAGCACAGCCGCCTTGCCCATGCTGAAGCAGAGCAATGGCA
  GCATTGCCGTCATCTCCTCCTTGGCTGGGAAAATGACCCAGCCTATGATTGCTCCCTACTCTGCAAGCAA
  GTTTGCTCTGGATGGGTTCTTTTCCACCATTAGAACAGAACTCTACATAACCAAGGTCAACGTGTCCATC
  ACTCTCTGTGTCCTTGGCCTCATAGACACAGAAACAGCTATGAAGGAAATCTCTGGGATAATTAACGCCC
  AAGCTTCTCCCAAGGAGGAGTGCGCCCTGGAGATCATCAAAGGCACAGCTCTACGCAAAAGCGAGGTGTA
  CTATGACAAATCGCCTTTGACTCCAATCCTGCTTGGGAACCCAGGAAGGAAGATCATGGAATTTTTTTCA
  TTACGATATTATAATAAGGACATGTTTGTAAGTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229057 representing NM_001044751
  Red=Cloning site Green=Tags(s)
  
  MAVMKNYLLPILVLFLAYYYYSTNEEFRPEMLQGKKVIVTGASKGIGREMAYHLSKMGAHVVLTARSEEG
  LQKVVSRCLELGAASAHYIAGTMEDMTFAEQFIVKAGKLMGGLDMLILNHITQTSLSLFHDDIHSVRRVM
  EVNFLSYVVMSTAALPMLKQSNGSIAVISSLAGKMTQPMIAPYSASKFALDGFFSTIRTELYITKVNVSI
  TLCVLGLIDTETAMKEISGIINAQASPKEECALEIIKGTALRKSEVYYDKSPLTPILLGNPGRKIMEFFS
  LRYYNKDMFVSN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001044751
• ORF Size: 876 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001044751.1, NP_001038216.1
• RefSeq Size: 1355
• RefSeq ORF: 879
• Locus ID: 15483
• MW: 32.4 kDa
• Gene Summary: Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. [UniProtKB/Swiss-Prot Function]