Crhr2 (NM_001288620) Mouse Tagged ORF Clone

CAT#: MR229138

Crhr2 (myc-DDK-tagged) - Mouse corticotropin releasing hormone receptor 2 (Crhr2), transcript variant 4

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Crhr2
• Synonyms: CRF-R2; Crfr2; CRFR2alpha; CRFR2beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229138 representing NM_001288620
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCAAGTACAACACGACCCGGAATGCCTACAGAGAGTGCCTGGAGAACGGGACCTGGGCCTCAAG
  GGTCAACTACTCACACTGCGAACCCATTTTGGATGACAAGGAGTATCCGCTGCCTGAGGAATGTGATCCA
  CTGGAACCTCATCACCACCTTCATTCTGAGAAACATCGCGTGGTTCCTGCTGCAACTCATCGACCACGAA
  GTGCACGAGGGCAATGAGGTCTGGTGCCGCTGCATCACCACCATCTTCAACTATTTTGTGGTCACCAACT
  TCTTCTGGATGTTTGTGGAGGGCTGCTACCTGCACACGGCCATTGTCATGACGTACTCCACAGAGCACCT
  GCGCAAGTGGCTTTTCCTCTTCATTGGATGGTGCATTCCCTGCCCTATCATCATCGCCTGGGCAGTTGGC
  AAACTCTACTATGAGAATGAGCAGTGCTGGTTTGGCAAGGAAGCTGGTGATTTGGTGGACTACATCTACC
  AGGGCCCCGTCATGCTTGTGCTGTTGATCAATTTTGTATTTCTGTTTAACATCGTCAGGATCCTGATGAC
  GAAGTTACGAGCATCCACCACGTCCGAGACAATCCAATACAGGAAGGCAGTGAAGGCCACGCTGGTCCTC
  CTCCCCCTGTTGGGCATCACCTACATGCTCTTCTTTGTCAATCCTGGCGAGGACGACCTGTCCCAGATTG
  TGTTCATCTACTTCAACTCTTTCCTGCAGTCCTTCCAGGGTTTCTTTGTGTCCGTTTTCTACTGCTTCTT
  CAATGGAGAGGTGCGCGCGGCCCTGAGAAAGCGGTGGCACCGCTGGCAGGACCACCACGCCCTCCGGGTG
  CCTGTGGCCCGGGCCATGTCCATCCCTACGTCGCCCACCAGGATCAGCTTCCACAGCATCAAGCAGACAG
  CTGCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229138 representing NM_001288620
  Red=Cloning site Green=Tags(s)
  
  MASSTTRPGMPTESAWRTGPGPQGSTTHTANPFWMTRSIRCLRNVIHWNLITTFILRNIAWFLLQLIDHE
  VHEGNEVWCRCITTIFNYFVVTNFFWMFVEGCYLHTAIVMTYSTEHLRKWLFLFIGWCIPCPIIIAWAVG
  KLYYENEQCWFGKEAGDLVDYIYQGPVMLVLLINFVFLFNIVRILMTKLRASTTSETIQYRKAVKATLVL
  LPLLGITYMLFFVNPGEDDLSQIVFIYFNSFLQSFQGFFVSVFYCFFNGEVRAALRKRWHRWQDHHALRV
  PVARAMSIPTSPTRISFHSIKQTAAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288620
• ORF Size: 918 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001288620.1, NP_001275549.1
• RefSeq Size: 2497
• RefSeq ORF: 921
• Locus ID: 12922
• MW: 36.1 kDa
• Gene Summary: G-protein coupled receptor for CRH (corticotropin-releasing factor), UCN (urocortin), UCN2 and UCN3. Has high affinity for UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. [UniProtKB/Swiss-Prot Function]