Dao (NM_001286396) Mouse Tagged ORF Clone

CAT#: MR229387

Dao (myc-DDK-tagged) - Mouse D-amino acid oxidase (Dao), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dao
• Synonyms: AI987963; DAAO; DAMOX; Dao-1; Dao1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229387 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGTGGCCGTGATCGGAGCAGGAGTCATTGGGCTCTCCACAGCCCTCTGCATTCATGAGCGTTACC
  ACCCAACACAGCCACTGCACATGAAGATCTATGCAGATCGATTCACCCCGTTCACCACGAGCGATGTGGC
  CGCCGGCCTCTGGCAGCCTTATCTCTCTGACCCCAGCAACCCTCAGGAGGCGGAGTGGAGCCAGCAAACG
  TTTGATTACCTGCTGAGCTGCCTCCATTCTCCAAACGCTGAAAAAATGGGCCTGGCCCTAATCTCAGGCT
  ACAACCTCTTCCGAGATGAAGTTCCGGACCCTTTCTGGAAAAACGCAGTTCTGGGATTCCGGAAGCTGAC
  CCCCAGTGAGATGGACCTGTTCCCTGATTATGGCTACGGCTGGTTCAATACAAGCCTCCTTCTAGAGGGG
  AAGAGCTACCTGCCATGGCTAACTGAGAGGTTAACTGAGAGGGGAGTGAAGCTTATCCATCGGAAGGTGG
  AGTCTCTCGAAGAGGTGGCAAGAGGAGTGGATGTGATTATCAACTGCACCGGGGTGTGGGCCGGGGCCCT
  GCAAGCAGATGCCTCCCTGCAGCCAGGCCGGGGCCAGATCATCCAGGTGGAGGCCCCTTGGATTAAACAC
  TTCATCCTCACCCATGATCCTAGCCTTGGTATCTACAACTCTCCGTACATCATCCCAGGTTCCAAGACAG
  TTACGCTCGGGGGTATATTCCAGCTGGGGAACTGGAGCGGGTTAAACAGCGTCCGTGACCACAATACCAT
  TTGGAAGAGCTGCTGTAAACTGGAGCCCACCCTGAAGAATGCAAGAATTGTGGGTGAACTCACTGGCTTC
  CGGCCAGTCCGGCCTCAGGTCCGGCTAGAAAGAGAATGGCTTCATTTTGGATCTTCAAGTGCAGAGGTCA
  TCCACAACTATGGTCATGGAGGTTACGGGCTCACAATCCACTGGGGTTGTGCAATGGAGGCGGCCAACCT
  CTTCGGGAAAATTCTAGAGGAAAAGAAGTTGTCCAGGTTGCCTCCCTCCCACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229387 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRVAVIGAGVIGLSTALCIHERYHPTQPLHMKIYADRFTPFTTSDVAAGLWQPYLSDPSNPQEAEWSQQT
  FDYLLSCLHSPNAEKMGLALISGYNLFRDEVPDPFWKNAVLGFRKLTPSEMDLFPDYGYGWFNTSLLLEG
  KSYLPWLTERLTERGVKLIHRKVESLEEVARGVDVIINCTGVWAGALQADASLQPGRGQIIQVEAPWIKH
  FILTHDPSLGIYNSPYIIPGSKTVTLGGIFQLGNWSGLNSVRDHNTIWKSCCKLEPTLKNARIVGELTGF
  RPVRPQVRLEREWLHFGSSSAEVIHNYGHGGYGLTIHWGCAMEAANLFGKILEEKKLSRLPPSHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286396
• ORF Size: 1038 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286396.1, NP_001273325.1
• RefSeq Size: 1859 bp
• RefSeq ORF: 1038 bp
• Locus ID: 13142
• MW: 38.7 kDa
• Gene Summary: Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids. [UniProtKB/Swiss-Prot Function]