Fut2 (NM_001271993) Mouse Tagged ORF Clone

CAT#: MR229401

Fut2 (myc-DDK-tagged) - Mouse fucosyltransferase 2 (Fut2), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fut2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229401 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGTGCCCAGGTACCTTTCTCCTTTCCTCTGGCCCACTTCCTCATCTTTGTCTTTGTGACTTCCA
  CCATCATCCACCTCCAGCAACGAATAGTGAAGCTCCAAACCCTGTCAGAGAAGGAATTACAGGCGGTTCA
  AATGTCCTCACCAAACGCGGCAAGAACAGACATGCAGCAGAGTGCCAAGCTGCAGGGCATATTCACGATC
  AATTCCATCGGCCGCCTGGGGAACCAGATGGGCGAATATGCTACATTGTTTGCACTGGCCAGGATGAACG
  GTCGGCTTGCCTTCATCCCTGAATCCATGCACAACGCTCTAGCGCCCATCTTCAGGATCAGTCTCCCGGT
  GTTACACAGCGACACAGCCAGAAGGATCCCGTGGCAGAATTACCACCTCAACGACTGGATGGAGGAGCGT
  TACCGCCACATCCCGGGGCAGTATGTGCGTTTCACGGGATACCCGTGCTCCTGGACCTTCTACCACCACC
  TGCGCCCAGAGATCCTGAAGGAGTTCACCCTGCACGACCATGTGCGTGAGGAGGCCCAGGCTTTCCTGCG
  TGGCCTGCGGGTGAATGGGAGCCAGCCGAGTACCTTTGTGGGTGTCCATGTGCGCCGAGGGGACTATGTG
  CATGTCATGCCCAAGGTGTGGAAGGGCGTGGTGGCTGACCGGGGTTACCTAGAAAAGGCCCTGGACAGGT
  TCCGGGCACGCTATTCATCTCCAGTCTTCGTGGTTACAAGCAACGGTATGGCCTGGTGCCGGGAGAACAT
  CAACACCTCCCTAGGAGACGTGGTGTTTGCGGGCAATGGTATTGAGGGCTCACCAGCCAAGGACTTCGCG
  CTCCTCACCCAGTGCAACCACACCATCATGACCATTGGAACCTTTGGGATTTGGGCTGCCTACCTGGCAG
  GTGGTGATACCATCTACCTAGCCAACTACACCCTTCCGGATTCTCCGTTCCTCAAAATCTTTAAGCCAGC
  AGCAGCCTTCCTCCCCGAGTGGATGGGCATCCCCGCAGACCTGTCCCCACTCCTTAAGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229401 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASAQVPFSFPLAHFLIFVFVTSTIIHLQQRIVKLQTLSEKELQAVQMSSPNAARTDMQQSAKLQGIFTI
  NSIGRLGNQMGEYATLFALARMNGRLAFIPESMHNALAPIFRISLPVLHSDTARRIPWQNYHLNDWMEER
  YRHIPGQYVRFTGYPCSWTFYHHLRPEILKEFTLHDHVREEAQAFLRGLRVNGSQPSTFVGVHVRRGDYV
  HVMPKVWKGVVADRGYLEKALDRFRARYSSPVFVVTSNGMAWCRENINTSLGDVVFAGNGIEGSPAKDFA
  LLTQCNHTIMTIGTFGIWAAYLAGGDTIYLANYTLPDSPFLKIFKPAAAFLPEWMGIPADLSPLLKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271993
• ORF Size: 1044 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001271993.1, NP_001258922.1
• RefSeq Size: 3073
• RefSeq ORF: 1044
• Locus ID: 14344
• MW: 39.2 kDa
• Gene Summary: This gene is one of three genes in mouse which encode a galactoside 2-L-fucosyltransferase. These genes differ in their developmental- and tissue-specific expression. The encoded type II membrane protein is anchored in the Golgi apparatus and controls the final step in the creation of alpha (1,2) fucosylated carbhohydrates by the addition of a terminal fucose in an alpha (1,2) linkage. This enzyme is involved in the synthesis of the Lewis antigen as well as the H-antigen, a precursor of the A and B antigens of the ABH histo-blood group. The biological function of the fucosylated carbhohydrate products is thought to involve cell-adhesion and interactions with microorganisms. Disruption of this gene results in altered glycosylation of gastric mucosa and uterine epithelia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]