Stk11 (NM_001301853) Mouse Tagged ORF Clone

CAT#: MR229772

Stk11 (myc-DDK-tagged) - Mouse serine/threonine kinase 11 (Stk11), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Stk11
• Synonyms: AA408040; Lkb1; Par-4; R75140
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229772 representing NM_001301853
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGTGGCGGACCCCGAGCCGTTGGGCCTTTTCTCCGAGGGCGAGCTGATGTCGGTGGGCATGGACA
  CCTTCATCCACCGCATCGACTCCACCGAGGTAATCTACCAGCCGCGCCGCAAACGCGCCAAGCTCATCGG
  CAAGTACCTGATGGGGGACCTGCTCGGGGAGGGCTCGTACGGCAAGGTGAAGGAGGTGCTGGACTCCGAG
  ACCTTATGCCGCAGGGCGGTCAAGATCCTCAAGAAGAAAAAGCTGCGCAGGATCCCCAATGGAGAGGCCA
  ACGTCAAGAAGGAGATCCAGCTGCTGCGGCGGCTGCGGCATCGGAATGTGATCCAGCTTGTGGACGTGCT
  GTACAATGAGGAGAAGCAGAAGATGTATATGGTGATGGAGTACTGCGTATGTGGCATGCAGGAGATGCTG
  GACAGTGTGCCGGAGAAGCGCTTCCCTGTGTGCCAAGCTCATGGGTACTTCCGCCAGCTGATTGACGGCC
  TGGAATACCTACACAGCCAGGGCATTGTTCACAAGGACATCAAGCCGGGCAACCTGCTACTCACCACCAA
  TGGCACACTCAAGATCTCCGACCTCGGTGTTGCCGAGGCCCTGCACCCTTTCGCTGTGGATGACACCTGC
  CGGACAAGCCAGGGCTCCCCGGCCTTCCAGCCTCCTGAGATTGCCAATGGACTGGACACCTTTTCAGGTT
  TCAAGGTGGACATCTGGTCAGCTGGGGTCACACTTTACAACATCACCACGGGCCTGTACCCATTTGAGGG
  GGACAATATCTACAAGCTCTTTGAGAACATTGGGAGAGGAGACTTCACCATCCCTTGTGACTGCGGCCCA
  CCACTCTCTGACCTACTCCGAGGGATGTTGGAGTATGAGCCGGCCAAGAGGTTCTCCATCCGACAGATTA
  GGCAGCACAGCTGGTTCCGGAAGAAACACCCTCTGGCTGAGGCGCTCGTACCTATCCCACCAAGCCCAGA
  CACTAAGGACCGCTGGCGCAGTATGACTGTAGTGCCCTACCTGGAGGACCTGCATGGCCGTGCGGAGGAG
  GAGGAGGAGGAAGACTTGTTTGACATTGAGGACGGCATTATCTACACCCAGGACTTCACAGTGCCTGGTG
  TCGAGGAGGCGGCCGAGGCAGGGCTTAGCGAGGATGCATGCGACACATGCATGTGGAAGAGCCAGGGCGC
  AGGCCTTCCTGGAGAGGAGCCCGAGGAGGGGTTTGGGGCTTTAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229772 representing NM_001301853
  Red=Cloning site Green=Tags(s)
  
  MDVADPEPLGLFSEGELMSVGMDTFIHRIDSTEVIYQPRRKRAKLIGKYLMGDLLGEGSYGKVKEVLDSE
  TLCRRAVKILKKKKLRRIPNGEANVKKEIQLLRRLRHRNVIQLVDVLYNEEKQKMYMVMEYCVCGMQEML
  DSVPEKRFPVCQAHGYFRQLIDGLEYLHSQGIVHKDIKPGNLLLTTNGTLKISDLGVAEALHPFAVDDTC
  RTSQGSPAFQPPEIANGLDTFSGFKVDIWSAGVTLYNITTGLYPFEGDNIYKLFENIGRGDFTIPCDCGP
  PLSDLLRGMLEYEPAKRFSIRQIRQHSWFRKKHPLAEALVPIPPSPDTKDRWRSMTVVPYLEDLHGRAEE
  EEEEDLFDIEDGIIYTQDFTVPGVEEAAEAGLSEDACDTCMWKSQGAGLPGEEPEEGFGALV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301853
• ORF Size: 1236 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001301853.1, NP_001288782.1
• RefSeq Size: 2054
• RefSeq ORF: 1239
• Locus ID: 20869
• MW: 46.9 kDa
• Gene Summary: This gene encodes a member of the serine/threonine kinase family. The encoded protein, a known tumor suppressor, activates (via phosphorylation) adenine monophosphate-activated protein kinase (AMPK) and AMPK-related kinase proteins. This upstream regulation of the AMPK pathway is thought to regulate a number of different processes, including cell metabolism, cell polarity, apoptosis and DNA damage response. Mutations in a similar gene in human have been associated with Peutz-Jeghers syndrome. Alternative splicing results in multiple transcript variants, including the S isoform which plays a potential role in spermiogenesis. [provided by RefSeq, Sep 2014]