Kcnk2 (NM_001281847) Mouse Tagged ORF Clone

CAT#: MR229782

Kcnk2 (myc-DDK-tagged) - Mouse potassium channel, subfamily K, member 2 (Kcnk2), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnk2
• Synonyms: A430027H14Rik; AI848635; TREK-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229782 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGCAGTGGCGGCCCCTGACTTGCTGGATCCCAAGTCTGCTGCTCAGAACTCCAAACCGAGGCTCT
  CATTCTCCTCAAAACCCACCGTGCTTGCTTCCCGGGTGGAGAGTGACTCGGCCATTAATGTTATGAAATG
  GAAGACAGTCTCCACGATTTTCCTGGTGGTCGTCCTCTACCTGATCATCGGAGCCACGGTGTTCAAGGCA
  TTGGAGCAGCCTCAGGAGATTTCCCAGAGGACCACCATTGTGATCCAGAAGCAGACCTTCATAGCCCAGC
  ATGCCTGCGTCAACTCCACCGAGCTGGACGAACTCATCCAGCAAATAGTGGCAGCAATAAACGCAGGGAT
  TATCCCCTTAGGAAACAGCTCCAATCAAGTTAGTCACTGGGACCTCGGAAGCTCTTTCTTCTTTGCTGGT
  ACTGTTATCACAACCATAGGATTTGGAAACATCTCCCCACGAACTGAAGGTGGAAAAATATTCTGCATCA
  TCTATGCCTTGCTGGGAATTCCCCTCTTTGGCTTTCTACTGGCTGGGGTTGGTGATCAGCTAGGAACTAT
  ATTTGGAAAAGGAATTGCCAAAGTGGAAGACACATTTATTAAGTGGAATGTTAGTCAGACGAAGATTCGT
  ATCATCTCCACCATCATCTTCATCCTGTTTGGCTGTGTCCTCTTTGTGGCTCTCCCTGCGGTCATATTCA
  AGCACATAGAAGGCTGGAGCGCCCTGGACGCTATCTATTTTGTGGTTATCACTCTGACGACCATTGGATT
  TGGAGACTACGTGGCAGGTGGATCAGACATTGAATATCTGGACTTCTACAAGCCTGTGGTGTGGTTCTGG
  ATCCTCGTTGGGCTGGCCTACTTTGCAGCTGTTCTGAGCATGATTGGGGACTGGCTACGGGTGATCTCTA
  AGAAGACGAAGGAAGAGGTGGGAGAGTTCAGAGCGCATGCCGCTGAGTGGACAGCCAATGTCACGGCCGA
  GTTCAAGGAAACGAGGAGGCGGCTGAGCGTGGAGATCTACGACAAGTTCCAGCGTGCCACATCCGTGAAG
  CGGAAGCTCTCCGCAGAGCTGGCGGGCAACCACAACCAGGAACTGACTCCGTGTAGGAGGACCCTGTCTG
  TGAACCACCTGACCAGCGAGAGGGAAGTCCTGCCTCCCTTGCTGAAGGCTGAGAGCATCTATCTGAACGG
  TCTGACACCACACTGTGCTGGTGAGGACATAGCTGTCATTGAGAACATGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229782 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGAVAAPDLLDPKSAAQNSKPRLSFSSKPTVLASRVESDSAINVMKWKTVSTIFLVVVLYLIIGATVFKA
  LEQPQEISQRTTIVIQKQTFIAQHACVNSTELDELIQQIVAAINAGIIPLGNSSNQVSHWDLGSSFFFAG
  TVITTIGFGNISPRTEGGKIFCIIYALLGIPLFGFLLAGVGDQLGTIFGKGIAKVEDTFIKWNVSQTKIR
  IISTIIFILFGCVLFVALPAVIFKHIEGWSALDAIYFVVITLTTIGFGDYVAGGSDIEYLDFYKPVVWFW
  ILVGLAYFAAVLSMIGDWLRVISKKTKEEVGEFRAHAAEWTANVTAEFKETRRRLSVEIYDKFQRATSVK
  RKLSAELAGNHNQELTPCRRTLSVNHLTSEREVLPPLLKAESIYLNGLTPHCAGEDIAVIENMK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001281847
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001281847.1, NP_001268776.1
• RefSeq Size: 3314 bp
• RefSeq ORF: 1245 bp
• Locus ID: 16526
• MW: 45.6 kDa