Ddx6 (NM_181324) Mouse Tagged ORF Clone

CAT#: MR230143

Ddx6 (myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (Ddx6), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ddx6
• Synonyms: 1110001P04Rik; C430015D01Rik; E230023J21Rik; HLR2; mRCK/P54; p54; rck
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230143 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCACGGCCAGAACAGAGAACCCTGTTATCATGGGTCTGTCCAGTCAGAACGGCCAGCTGAGGGGCC
  CTGTGAAGGCCAGTGCTGGCCCTGGAGGAGGGGGCACACAGCCACAGCCACAGCTGAACCAGTTGAAGAA
  CACCAGCACAATCAATAATGGCACTCCACAGCAAGCCCAGAGCATGGCCGCCACTATTAAACCTGGTGAT
  GACTGGAAAAAGACATTAAAACTCCCTCCAAAGGATCTAAGAATCAAAACTTCGGATGTGACCTCCACAA
  AAGGAAATGAGTTTGAAGATTACTGTTTGAAACGAGAGTTGCTAATGGGAATTTTTGAAATGGGCTGGGA
  AAAACCATCTCCTATCCAGGAGGAGAGCATTCCCATTGCTTTATCTGGTAGGGATATCTTAGCTAGAGCA
  AAAAATGGAACAGGCAAAAGCGGTGCCTACCTCATCCCCTTACTTGAAAGGCTGGACCTGAAGAAGGACA
  ATATTCAAGCAATGGTGATTGTTCCCACTAGAGAACTTGCTCTACAGGTCAGTCAGATTTGCATCCAGGT
  CAGCAAACACATGGGAGGGGCCAAAGTGATGGCAACCACCGGAGGAACCAATTTACGAGATGACATAATG
  AGGCTTGATGATACAGTGCATGTGGTGATCGCTACCCCTGGCAGGATCCTGGATCTCATCAAGAAAGGCG
  TGGCAAAGGTTGACCATGTCCAGATGATAGTGCTAGATGAGGCAGATAAATTGTTGTCACAGGATTTTGT
  GCAGATAATGGAGGATATTATTCTCACACTACCTAAAAATAGGCAGATTTTACTATACTCCGCTACTTTC
  CCTCTTAGTGTACAAAAGTTTATGAATTCCCATCTGCAAAAACCCTACGAGATAAACCTGATGGAGGAAC
  TAACTCTGAAAGGAGTAACTCAGTACTACGCATATGTAACGGAGCGCCAAAAAGTGCACTGCCTCAATAC
  ACTTTTCTCCAGGCTTCAGATAAACCAGTCCATCATTTTCTGCAACTCCTCCCAGAGAGTCGAGCTACTC
  GCCAAGAAGATTTCTCAGCTGGGCTACTCTTGCTTTTATATCCATGCTAAAATGAGGCAGGAACATCGAA
  ATCGTGTCTTTCATGATTTCCGAAATGGCTTATGCCGCAATCTTGTTTGCACTGATCTGTTTACCCGAGG
  TATTGATATACAAGCTGTGAATGTGGTAATAAACTTTGACTTCCCAAAGCTGGCAGAGACCTATCTTCAT
  CGTATTGGAAGATCAGGTCGCTTTGGTCATCTTGGCTTAGCCATCAACTTGATCACTTATGATGATCGCT
  TCAACCTGAAGAGTATTGAGGAGCAGCTGGGGACAGAGATCAAACCCATCCCAAGCAACATCGACAAGAG
  CCTGTATGTGGCAGAATACCACAGTGAGCCTGCAGAAGACGAGAAACCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230143 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTARTENPVIMGLSSQNGQLRGPVKASAGPGGGGTQPQPQLNQLKNTSTINNGTPQQAQSMAATIKPGD
  DWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARA
  KNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIM
  RLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATF
  PLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELL
  AKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLH
  RIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPAEDEKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181324
• ORF Size: 1449 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_181324.1, NM_181324.2, NM_181324.3, NP_851841.2
• RefSeq Size: 6033
• RefSeq ORF: 1452
• Locus ID: 13209
• MW: 54.2 kDa