F10 (NM_001242368) Mouse Tagged ORF Clone

CAT#: MR230216

F10 (myc-DDK-tagged) - Mouse coagulation factor X (F10), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: F10
• Synonyms: Cf10; fX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230216 representing NM_001242368
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGTCACAGCCACTGCCGTCCTTGACCACACCATGGGGAGCCCGGTGCAACTCAGCCTGCTCTGTG
  TTGTCCTGGCCAGCCTCCTGCTCCCTGGGAAAGGTGTGTTTATTAACCGGGAACGTGCCAACAATGTCCT
  GGCGAGGACTCGGAGGGCAAACTCATTTTTTGAAGAGTTCAAGAAAGGAAATCTGGAAAGAGAGTGTATG
  GAAGAAATTTGTTCTTATGAAGAGGTCCGTGAAATCTTCGAGGACGACGAGAAGACGAAAGAATACTGGA
  CCAAATATAAAGACGGCGACCAGTGTGAAAGCAGCCCTTGCCAGAACCAAGGAGCGTGTCGAGATGGCAT
  CGGGGGTTACACGTGCACCTGCTCGGAGGGATTTGAAGGCAAAAACTGTGAGCTCTTTGTTCGGAAACTC
  TGCCGCCTAGACAACGGAGACTGTGACCAGTTCTGCAGAGAAGAGCAGAACTCAGTGGTGTGCTCCTGCG
  CCAGCGGTTACTTCCTGGGTAATGATGGCAAGTCTTGCATCTCCACAGCTCCCTTCCCCTGTGGAAAAAT
  CACTACAGGACGTAGGAAGAGGTCTGTGGCCCTAAACACCAGCGACAGTGAGCTTGACCTTGAAGACGCC
  CTGCTTGATGAGGATTTCCTGTCCCCTACGGAGAATCCTATTGAACTGCTCAACCTCAACGAGACACAGC
  CTGAGAGGAGCAGCGATGACCTTGTTCGGATTGTGGGTGGCCGGGAATGCAAGGATGGAGAATGTCCCTG
  GCAGGCTCTGCTCATTAACGAAGACAATGAAGGGTTCTGTGGGGGCACCATCTTGAATGAGTTCTACATC
  CTCACTGCTGCCCACTGTCTCCATCAGGCCAGGCGATTCAAGGTGAGGGTAGGTGATCGGAACACAGAGA
  AGGAAGAAGGCAACGAGATGGTGCACGAGGTGGACGTGGTCATTAAGCACAACAAGTTTCAGAGGGACAC
  CTACGACTATGATATCGCCGTGCTGAGGCTGAAGACTCCCATCACGTTCCGGATGAACGTGGCCCCTGCC
  TGCCTGCCTCAGAAAGACTGGGCCGAGTCCACACTGATGACACAGAAGACGGGCATCGTGAGCGGGTTTG
  GACGCACGCATGAGAAGGGCCGCCAGTCGAACATCCTGAAGATGCTGGAGGTACCCTACGTGGATCGCAA
  CACCTGCAAGCTCTCCACCAGCTTCAGCATCACACAGAATATGTTCTGTGCGGGCTATGAGGCCAAGTTA
  GAGGATGCCTGCCAGGGGGACAGTGGTGGCCCCCATGTCACACGGTTCAAAAATACCTACTATGTGACCG
  GCATTGTCAGCTGGGGAGAGGGGTGTGCAAGGAAAGGGAAATATGGCATCTACACAAAGGTCACGACCTT
  CCTCAAGTGGATTGACAGGTCCATGAAAGCCAGGGTGGGACCCACAGCCGAGACCCCAAGGACAGCAGGT
  CCGCCCAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230216 representing NM_001242368
  Red=Cloning site Green=Tags(s)
  
  MPVTATAVLDHTMGSPVQLSLLCVVLASLLLPGKGVFINRERANNVLARTRRANSFFEEFKKGNLERECM
  EEICSYEEVREIFEDDEKTKEYWTKYKDGDQCESSPCQNQGACRDGIGGYTCTCSEGFEGKNCELFVRKL
  CRLDNGDCDQFCREEQNSVVCSCASGYFLGNDGKSCISTAPFPCGKITTGRRKRSVALNTSDSELDLEDA
  LLDEDFLSPTENPIELLNLNETQPERSSDDLVRIVGGRECKDGECPWQALLINEDNEGFCGGTILNEFYI
  LTAAHCLHQARRFKVRVGDRNTEKEEGNEMVHEVDVVIKHNKFQRDTYDYDIAVLRLKTPITFRMNVAPA
  CLPQKDWAESTLMTQKTGIVSGFGRTHEKGRQSNILKMLEVPYVDRNTCKLSTSFSITQNMFCAGYEAKL
  EDACQGDSGGPHVTRFKNTYYVTGIVSWGEGCARKGKYGIYTKVTTFLKWIDRSMKARVGPTAETPRTAG
  PPN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242368
• ORF Size: 1479 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001242368.1, NP_001229297.1
• RefSeq Size: 2693
• RefSeq ORF: 1482
• Locus ID: 14058
• MW: 55.7 kDa
• Gene Summary: This gene encodes factor X, a component of both the intrinsic and extrinsic blood coagulation pathways. The encoded protein is a zymogen that undergoes further processing in a vitamin K-dependent manner to generate mature factor X, a heterodimer comprised of disulfide-linked heavy and light chains. The mature factor X is proteolytically activated either by factor IXa (intrinsic pathway) or factor VIIa (extrinsic pathway) to form factor Xa serine endopeptidase. Activated factor Xa catalyzes the conversion of prothrombin to thrombin. A complete lack of the encoded protein is fatal to mice. A severe deficiency of the encoded protein in mice causes age-dependent iron deposition and cardiac fibrosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]