Mavs (NM_001206385) Mouse Tagged ORF Clone

CAT#: MR230282

Mavs (myc-DDK-tagged) - Mouse mitochondrial antiviral signaling protein (Mavs), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mavs
• Synonyms: cardif; D430028G21Rik; IPS-1; Visa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230282 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACATTTGCTGAGGACAAGACCTATAAGTATATCCGAGACAACCACAGCAAGTTTTGCTGTGTTGACG
  TTCTGGAGATCCTGCCTTACCTGTCCTGCCTCACAGCTAGTGACCAGGATCGACTGCGGGCTTCCTACAG
  GCAGATCGGGAACCGGGACACACTCTGGGGACTCTTCAATAATCTCCAGCGCCGGCCTGGCTGGGTGGAG
  GTCTTCATCCGGGCACTGCAGATCTGTGAGCTGCCTGGGCTGGCTGATCAAGTGACTCGAGTTTATCAGA
  GCTACCTGCCTCCGGGGACCTCACTCCGCTCCCTAGAGCCACTGCAGTTACCAGACTTTCCTGCTGCGGT
  TTCTGGACCCTCTGCATTTGCGCCAGGTCACAACATCCCTGACCATGGCTTACGAGAGACACCAAGTTGC
  CCCAAGCCTGTCCAGGACACCCAGCCACCAGAGTCCCCAGTAGAGAATTCAGAGCAACTCCTCCAGACCA
  ACTCCGGGGCCGTCGCGAGGATGTCTGGTGGCTCTTTGATACCCTCTCCTAACCAGCAGGCTCTCAGCCC
  TCAGCCCTCCAGAGAGCATCAAGAGCAAGAACCAGAACTGGGTGGCGCCCACGCAGCAAATGTTGCCTCT
  GTTCCCATAGCAACCTATGGACCTGTGTCTCCAACCGTTTCCTTCCAGCCCCTTCCACGTACTGCCCTGA
  GGACAAACCTCTTGTCTGGGGTCACAGTATCAGCCCTATCTGCTGATACCTCTTTGTCCTCCTCGTCCAC
  TGGATCAGCTTTTGCAAAGGGAGCTGGTGACCAGGCCAAAGCTGCCACCTGTTTCAGTACTACACTCACC
  AATTCTGTGACTACCAGCTCAGTGCCTTCTCCCAGATTGGTCCCAGTAAAAACCATGTCTTCCAAGTTGC
  CCCTCAGTTCAAAGTCCACTGCTGCGATGACGTCTACTGTGCTCACCAATACAGCGCCATCAAAATTACC
  CAGCAACTCAGTGTATGCGGGCACAGTGCCATCCAGAGTGCCTGCTAGTGTGGCCAAAGCACCTGCCAAC
  ACAATACCACCTGAGAGGAACAGCAAGCAAGCCAAGGAGACCCCGGAGGGTCCAGCAACCAAAGTCACCA
  CTGGAGGCAACCAGACTGGACCAAATAGCAGTATCAGGAGCTTGCACTCTGGACCAGAGATGAGCAAGCC
  AGGTGTGCTGGTATCCCAGTTGGACGAGCCATTCTCAGCCTGCTCTGTGGACCTTGCCATTAGCCCTAGC
  AGCTCCTTGGTCTCAGAACCCAACCATGGTCCAGAGGAGAATGAGTATTCGTCCTTTAGAATCCAGGTAG
  ACGAAAGCCCCAGTGCTGATCTATTAGGAAGCCCTGAGCCACTAGCCACCCAGCAGCCCCAAGAAGAGGA
  AGAACATTGTGCCAGTTCAATGCCCTGGGCTAAGTGGCTTGGGGCCACCAGTGCACTCTTGGCTGTATTC
  CTGGCAGTGATGCTGTACCGTAGTAGGCGCCTGGCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230282 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTFAEDKTYKYIRDNHSKFCCVDVLEILPYLSCLTASDQDRLRASYRQIGNRDTLWGLFNNLQRRPGWVE
  VFIRALQICELPGLADQVTRVYQSYLPPGTSLRSLEPLQLPDFPAAVSGPSAFAPGHNIPDHGLRETPSC
  PKPVQDTQPPESPVENSEQLLQTNSGAVARMSGGSLIPSPNQQALSPQPSREHQEQEPELGGAHAANVAS
  VPIATYGPVSPTVSFQPLPRTALRTNLLSGVTVSALSADTSLSSSSTGSAFAKGAGDQAKAATCFSTTLT
  NSVTTSSVPSPRLVPVKTMSSKLPLSSKSTAAMTSTVLTNTAPSKLPSNSVYAGTVPSRVPASVAKAPAN
  TIPPERNSKQAKETPEGPATKVTTGGNQTGPNSSIRSLHSGPEMSKPGVLVSQLDEPFSACSVDLAISPS
  SSLVSEPNHGPEENEYSSFRIQVDESPSADLLGSPEPLATQQPQEEEEHCASSMPWAKWLGATSALLAVF
  LAVMLYRSRRLAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001206385
• ORF Size: 1512 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001206385.1, NP_001193314.1
• RefSeq Size: 2871 bp
• RefSeq ORF: 1512 bp
• Locus ID: 228607
• MW: 53.4 kDa