Mier1 (NM_001286223) Mouse Tagged ORF Clone

CAT#: MR230313

Mier1 (myc-DDK-tagged) - Mouse mesoderm induction early response 1 homolog (Xenopus laevis (Mier1), transcript variant 5

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mier1
• Synonyms: 4933425I22Rik; 5830411K19Rik; er1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230313 representing NM_001286223
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCATCTGTTGAGTCTTCAAGTCCAGGAGGCTCAGCAACATCAGAGGACCATGAGTTTGATCCAT
  CAGCTGACATGCTGGTTCATGATTTTGATGATGAGCGAACATTAGAAGAAGAAGAAATGATGGAAGGAGA
  GACAAACTTCAGTTCTGAAATAGAGGATCTTGCGAGGGAAGGCGACATGCCAATCCACGAGCTGCTCAGC
  CTCTATGGTTATGACAGCACTGTTCGGTTACCTGAGGAAGAGGAGGAGGAAGAGGAGGAGGAGGAAGGTG
  AAGATGATGAAGATGCTGATAATGATGATAACAGTGGCTGTAGTGGAGAAAATAAAGAAGAGAATATAAA
  GGATTCATCGGGTCAGGAGGATGAAACTCAGTCTTCCAATGATGATCCCTCACAGTCTGTTACTTCCCAA
  GATGCTCAGGAAATAATCCGCCCACGTCGATGTAAATATTTTGATACAAATAGTGAAATAGAAGAAGAAT
  CTGAAGAAGATGAAGATTATATTCCCTCAGAAGACTGGAAAAAGGAAATCATGGTGGGCTCCATGTTTCA
  AGCTGAGATTCCAGTTGGTGTTTGTAGATACAAAGAAAATGAAAAAGTGTATGAAAATGATGATCAGCTT
  CTGTGGGATCCAGAGTGTTTACCAGAAGAGAAAGTGGTTGTCTTTCTTAAGGATGCGTCTAGAAGGACAG
  GGGATGAGAAAGGTGTGGAAGCAATCCCTGAAGGCTCGCATATCAAAGACAATGAGCAGGCTTTATATGA
  GTTGGTTAAATGCAGCTTTGATACGGAAGAAGCCTTGAGAAGACTGAGATTTAATGTCAAAGCAGCTCGA
  GAGGAGTTATCTGTGTGGACAGAGGAAGAGTGTAGAAATTTTGAGCAAGGGCTGAAGGCCTATGGAAAAG
  ATTTTCATCTGATTCAGGCTAATAAAGTCCGAACAAGATCAGTTGGTGAATGTGTAGCATTCTATTACAT
  GTGGAAAAAGTCTGAGCGCTATGATTTCTTTGCTCAGCAAACAAGGTTTGGAAAAAAGAAATATAATCTT
  CATCCTGGTGTAACGGATTACATGGATCGTCTTTTGGATGAAAGTGAAAGTGCTGCTTCTAGCCGAGCAC
  CATCCCCCCCTCCCACTGCCTCAAATAGCAGTAACAGCCAGTCCGAGAAAGAAGATGGCGCTGTCAGCAG
  TAGGAATCAGAACGGTGTCTCATCTAATGGACCAGGAGAAATACTAAACAAAGAAGAAGTAAAAGTTGAA
  GGGTTACATGTTAATGGACCAACAGGTGGAAATAAGAAACCACTTCTTACAGATATGGACACTAATGGTT
  ATGAAGCAAATAACCTGACCACTGACCCAAAACTTGCCCATATGACTGCAAGAAATGAAAATGATTTTGA
  TGAAAAAAATGAGAGACCTGCCAAAAGGCGGCGGATAAACAGCAGTGGGAAAGAAAGTCCGGGCTCATCT
  GAGTTTTTCCAGGAAGCAGTCTCACATGGGAAGTTTGAGGAACATGAAAACACGAATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230313 representing NM_001286223
  Red=Cloning site Green=Tags(s)
  
  MLPSVESSSPGGSATSEDHEFDPSADMLVHDFDDERTLEEEEMMEGETNFSSEIEDLAREGDMPIHELLS
  LYGYDSTVRLPEEEEEEEEEEEGEDDEDADNDDNSGCSGENKEENIKDSSGQEDETQSSNDDPSQSVTSQ
  DAQEIIRPRRCKYFDTNSEIEEESEEDEDYIPSEDWKKEIMVGSMFQAEIPVGVCRYKENEKVYENDDQL
  LWDPECLPEEKVVVFLKDASRRTGDEKGVEAIPEGSHIKDNEQALYELVKCSFDTEEALRRLRFNVKAAR
  EELSVWTEEECRNFEQGLKAYGKDFHLIQANKVRTRSVGECVAFYYMWKKSERYDFFAQQTRFGKKKYNL
  HPGVTDYMDRLLDESESAASSRAPSPPPTASNSSNSQSEKEDGAVSSRNQNGVSSNGPGEILNKEEVKVE
  GLHVNGPTGGNKKPLLTDMDTNGYEANNLTTDPKLAHMTARNENDFDEKNERPAKRRRINSSGKESPGSS
  EFFQEAVSHGKFEEHENTND
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286223
• ORF Size: 1530 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001286223.1, NP_001273152.1
• RefSeq Size: 4631
• RefSeq ORF: 1533
• Locus ID: 71148
• MW: 58.3 kDa