Mier1 (NM_001286222) Mouse Tagged ORF Clone

CAT#: MR230393

Mier1 (myc-DDK-tagged) - Mouse mesoderm induction early response 1 homolog (Xenopus laevis (Mier1), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mier1
• Synonyms: 4933425I22Rik; 5830411K19Rik; er1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230393 representing NM_001286222
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTATGTTTAATTGGTTTACAGACTGTCTCTGGATTCTTTTCCTGTCAAATTACAAGCCATCTGTTG
  AGTCTTCAAGTCCAGGAGGCTCAGCAACATCAGAGGACCATGAGTTTGATCCATCAGCTGACATGCTGGT
  TCATGATTTTGATGATGAGCGAACATTAGAAGAAGAAGAAATGATGGAAGGAGAGACAAACTTCAGTTCT
  GAAATAGAGGATCTTGCGAGGGAAGGCGACATGCCAATCCACGAGCTGCTCAGCCTCTATGGTTATGACA
  GCACTGTTCGGTTACCTGAGGAAGAGGAGGAGGAAGAGGAGGAGGAGGAAGGTGAAGATGATGAAGATGC
  TGATAATGATGATAACAGTGGCTGTAGTGGAGAAAATAAAGAAGAGAATATAAAGGATTCATCGGGTCAG
  GAGGATGAAACTCAGTCTTCCAATGATGATCCCTCACAGTCTGTTACTTCCCAAGATGCTCAGGAAATAA
  TCCGCCCACGTCGATGTAAATATTTTGATACAAATAGTGAAATAGAAGAAGAATCTGAAGAAGATGAAGA
  TTATATTCCCTCAGAAGACTGGAAAAAGGAAATCATGGTGGGCTCCATGTTTCAAGCTGAGATTCCAGTT
  GGTGTTTGTAGATACAAAGAAAATGAAAAAGTGTATGAAAATGATGATCAGCTTCTGTGGGATCCAGAGT
  GTTTACCAGAAGAGAAAGTGGTTGTCTTTCTTAAGGATGCGTCTAGAAGGACAGGGGATGAGAAAGGTGT
  GGAAGCAATCCCTGAAGGCTCGCATATCAAAGACAATGAGCAGGCTTTATATGAGTTGGTTAAATGCAGC
  TTTGATACGGAAGAAGCCTTGAGAAGACTGAGATTTAATGTCAAAGCAGCTCGAGAGGAGTTATCTGTGT
  GGACAGAGGAAGAGTGTAGAAATTTTGAGCAAGGGCTGAAGGCCTATGGAAAAGATTTTCATCTGATTCA
  GGCTAATAAAGTCCGAACAAGATCAGTTGGTGAATGTGTAGCATTCTATTACATGTGGAAAAAGTCTGAG
  CGCTATGATTTCTTTGCTCAGCAAACAAGGTTTGGAAAAAAGAAATATAATCTTCATCCTGGTGTAACGG
  ATTACATGGATCGTCTTTTGGATGAAAGTGAAAGTGCTGCTTCTAGCCGAGCACCATCCCCCCCTCCCAC
  TGCCTCAAATAGCAGTAACAGCCAGTCCGAGAAAGAAGATGGCGCTGTCAGCAGTAGGAATCAGAACGGT
  GTCTCATCTAATGGACCAGGAGAAATACTAAACAAAGAAGAAGTAAAAGTTGAAGGGTTACATGTTAATG
  GACCAACAGGTGGAAATAAGAAACCACTTCTTACAGATATGGACACTAATGGTTATGAAGCAAATAACCT
  GACCACTGACCCAAAACTTGCCCATATGACTGCAAGAAATGAAAATGATTTTGATGAAAAAAATGAGAGA
  CCTGCCAAAAGGCGGCGGATAAACAGCAGTGGGAAAGAAAGTCCGGGCTCATCTGAGTTTTTCCAGGAAG
  CAGTCTCACATGGGAAGTTTGAGGAACATGAAAACACGAATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230393 representing NM_001286222
  Red=Cloning site Green=Tags(s)
  
  MFMFNWFTDCLWILFLSNYKPSVESSSPGGSATSEDHEFDPSADMLVHDFDDERTLEEEEMMEGETNFSS
  EIEDLAREGDMPIHELLSLYGYDSTVRLPEEEEEEEEEEEGEDDEDADNDDNSGCSGENKEENIKDSSGQ
  EDETQSSNDDPSQSVTSQDAQEIIRPRRCKYFDTNSEIEEESEEDEDYIPSEDWKKEIMVGSMFQAEIPV
  GVCRYKENEKVYENDDQLLWDPECLPEEKVVVFLKDASRRTGDEKGVEAIPEGSHIKDNEQALYELVKCS
  FDTEEALRRLRFNVKAAREELSVWTEEECRNFEQGLKAYGKDFHLIQANKVRTRSVGECVAFYYMWKKSE
  RYDFFAQQTRFGKKKYNLHPGVTDYMDRLLDESESAASSRAPSPPPTASNSSNSQSEKEDGAVSSRNQNG
  VSSNGPGEILNKEEVKVEGLHVNGPTGGNKKPLLTDMDTNGYEANNLTTDPKLAHMTARNENDFDEKNER
  PAKRRRINSSGKESPGSSEFFQEAVSHGKFEEHENTND
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286222
• ORF Size: 1584 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001286222.1, NP_001273151.1
• RefSeq Size: 4835
• RefSeq ORF: 1587
• Locus ID: 71148
• MW: 60.6 kDa