Me1 (NM_001198933) Mouse Tagged ORF Clone

CAT#: MR230481

Me1 (myc-DDK-tagged) - Mouse malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Me1
• Synonyms: D9Ertd267e; Mdh-1; Mod-1; Mod1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230481 representing NM_001198933
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTGATCTGCGAAGACTTGGCTTTTACTCTGGAAGAGAGACAGCAGTTGAACATTCATGGATTGT
  TGCCGCCCTGCATCATCAGCCAGGAGCTCCAGGTCCTTAGAATAATTAAGAATTTCGAACGACTGAACTC
  TGACTTCGACAGGTATCTCCTGTTAATGGACCTGCAAGACAGAAATGAGAAGCTCTTCTACAGCGTGCTC
  ATGTCTGATGTTGAAAAGTTCATGCCTATTGTTTACACCCCCACCGTGGGCCTCGCATGCCAGCAGTACA
  GTTTGGCATTCCGGAAGCCAAGAGGCCTCTTTATTAGTATCCATGACAAAGGGCACATTGCTTCAGTTCT
  TAATGCATGGCCAGAGGATGTCGTCAAGGCTATTGTGGTAACTGATGGAGAGCGCATCCTTGGCTTGGGA
  GACCTTGGCTGTAATGGGATGGGCATCCCTGTGGGTAAACTGGCCCTGTACACGGCATGTGGAGGGGTGA
  ACCCACAACAGTGTCTACCCATCACTTTGGATGTGGGAACAGAAAATGAGGAGTTACTTAAGGATCCACT
  GTACATCGGGCTGCGGCACCGGCGAGTCAGAGGCCCTGAGTATGACGCCTTCCTGGATGAGTTCATGGAG
  GCAGCGTCTTCCAAATATGGCATGAATTGCCTTATTCAGTTTGAAGATTTTGCCAATCGGAATGCATTTC
  GTCTCCTGAACAAGTATCGAAACAAGTATTGCACATTTAACGATGATATTCAAGGAACAGCGTCTGTTGC
  GGTTGCGGGTCTCCTTGCAGCTCTTCGAATAACCAAGAACAAGCTCTCTGATCAGACAGTGCTGTTCCAG
  GGAGCTGGAGAGGCTGCCTTGGGGATTGCTCACTTGGTTGTTATGGCCATGGAGAAAGAAGGTTTATCAA
  AGGAGAATGCTAGAAAGAAGATATGGTTGGTTGACTCAAAAGGACTAATAGTTAAGGGTCGTGCATCTCT
  CACAGAAGAGAAAGAGGTGTTTGCCCATGAACATGAAGAAATGAAGAATCTGGAAGCCATTGTTCAAAAG
  ATAAAACCAACTGCCCTCATAGGAGTTGCTGCAATTGGTGGTGCTTTCACTGAACAAATTCTCAAGGATA
  TGGCTGCCTTCAACGAGCGGCCCATCATCTTTGCTTTGAGTAATCCGACCAGCAAAGCGGAGTGCTCTGC
  AGAGCAGTGCTACAAGGTGACCAAGGGACGTGCAATCTTTGCCAGCGGCAGTCCTTTTGATCCAGTCACT
  CTCCCAGATGGACGGACTCTGTTTCCTGGCCAAGGCAACAATTCCTACGTGTTCCCTGGAGTTGCTCTTG
  GGGTGGTGGCCTGCGGACTGAGACACATCGATGATAAGGTCTTCCTCACCACTGCTGAGGTCATATCTCA
  GCAAGTGTCAGATAAACACCTGCAAGAAGGCCGGCTCTATCCTCCTTTGAATACCATTCGAGGCGTTTCG
  TTGAAAATTGCAGTAAAGATTGTGCAAGATGCATACAAAGAAAAGATGGCCACTGTTTATCCTGAACCCC
  AAAACAAAGAAGAATTTGTCTCCTCCCAGATGTACAGCACTAATTATGACCAGATCCTACCTGATTGTTA
  TCCGTGGCCTGCAGAAGTCCAGAAAATACAGACCAAAGTCAACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230481 representing NM_001198933
  Red=Cloning site Green=Tags(s)
  
  MRLICEDLAFTLEERQQLNIHGLLPPCIISQELQVLRIIKNFERLNSDFDRYLLLMDLQDRNEKLFYSVL
  MSDVEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDKGHIASVLNAWPEDVVKAIVVTDGERILGLG
  DLGCNGMGIPVGKLALYTACGGVNPQQCLPITLDVGTENEELLKDPLYIGLRHRRVRGPEYDAFLDEFME
  AASSKYGMNCLIQFEDFANRNAFRLLNKYRNKYCTFNDDIQGTASVAVAGLLAALRITKNKLSDQTVLFQ
  GAGEAALGIAHLVVMAMEKEGLSKENARKKIWLVDSKGLIVKGRASLTEEKEVFAHEHEEMKNLEAIVQK
  IKPTALIGVAAIGGAFTEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYKVTKGRAIFASGSPFDPVT
  LPDGRTLFPGQGNNSYVFPGVALGVVACGLRHIDDKVFLTTAEVISQQVSDKHLQEGRLYPPLNTIRGVS
  LKIAVKIVQDAYKEKMATVYPEPQNKEEFVSSQMYSTNYDQILPDCYPWPAEVQKIQTKVNQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198933
• ORF Size: 1656 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001198933.1, NP_001185862.1
• RefSeq Size: 3335
• RefSeq ORF: 1659
• Locus ID: 17436
• MW: 61.9 kDa