Mier1 (NM_001286221) Mouse Tagged ORF Clone

CAT#: MR230501

Mier1 (myc-DDK-tagged) - Mouse mesoderm induction early response 1 homolog (Xenopus laevis (Mier1), transcript variant 4

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mier1
• Synonyms: 4933425I22Rik; 5830411K19Rik; er1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230501 representing NM_001286221
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCATCAGATGTTTATGTTTAATTGGTTTACAGACTGTCTCTGGATTCTTTTCCTGTCAAATTACAA
  GCCATCTGTTGAGTCTTCAAGTCCAGGGTGAATATAAGTGTGGCCTGCTCTGTGAAAACACTTCATTGTT
  GATTGCTAATGTCAGAAGAAAAGTTTCAGGAGGCTCAGCAACATCAGAGGACCATGAGTTTGATCCATCA
  GCTGACATGCTGGTTCATGATTTTGATGATGAGCGAACATTAGAAGAAGAAGAAATGATGGAAGGAGAGA
  CAAACTTCAGTTCTGAAATAGAGGATCTTGCGAGGGAAGGCGACATGCCAATCCACGAGCTGCTCAGCCT
  CTATGGTTATGACAGCACTGTTCGGTTACCTGAGGAAGAGGAGGAGGAAGAGGAGGAGGAGGAAGGTGAA
  GATGATGAAGATGCTGATAATGATGATAACAGTGGCTGTAGTGGAGAAAATAAAGAAGAGAATATAAAGG
  ATTCATCGGGTCAGGAGGATGAAACTCAGTCTTCCAATGATGATCCCTCACAGTCTGTTACTTCCCAAGA
  TGCTCAGGAAATAATCCGCCCACGTCGATGTAAATATTTTGATACAAATAGTGAAATAGAAGAAGAATCT
  GAAGAAGATGAAGATTATATTCCCTCAGAAGACTGGAAAAAGGAAATCATGGTGGGCTCCATGTTTCAAG
  CTGAGATTCCAGTTGGTGTTTGTAGATACAAAGAAAATGAAAAAGTGTATGAAAATGATGATCAGCTTCT
  GTGGGATCCAGAGTGTTTACCAGAAGAGAAAGTGGTTGTCTTTCTTAAGGATGCGTCTAGAAGGACAGGG
  GATGAGAAAGGTGTGGAAGCAATCCCTGAAGGCTCGCATATCAAAGACAATGAGCAGGCTTTATATGAGT
  TGGTTAAATGCAGCTTTGATACGGAAGAAGCCTTGAGAAGACTGAGATTTAATGTCAAAGCAGCTCGAGA
  GGAGTTATCTGTGTGGACAGAGGAAGAGTGTAGAAATTTTGAGCAAGGGCTGAAGGCCTATGGAAAAGAT
  TTTCATCTGATTCAGGCTAATAAAGTCCGAACAAGATCAGTTGGTGAATGTGTAGCATTCTATTACATGT
  GGAAAAAGTCTGAGCGCTATGATTTCTTTGCTCAGCAAACAAGGTTTGGAAAAAAGAAATATAATCTTCA
  TCCTGGTGTAACGGATTACATGGATCGTCTTTTGGATGAAAGTGAAAGTGCTGCTTCTAGCCGAGCACCA
  TCCCCCCCTCCCACTGCCTCAAATAGCAGTAACAGCCAGTCCGAGAAAGAAGATGGCGCTGTCAGCAGTA
  GGAATCAGAACGGTGTCTCATCTAATGGACCAGGAGAAATACTAAACAAAGAAGAAGTAAAAGTTGAAGG
  GTTACATGTTAATGGACCAACAGGTGGAAATAAGAAACCACTTCTTACAGATATGGACACTAATGGTTAT
  GAAGCAAATAACCTGACCACTGACCCAAAACTTGCCCATATGACTGCAAGAAATGAAAATGATTTTGATG
  AAAAAAATGAGAGACCTGCCAAAAGGCGGCGGATAAACAGCAGTGGGAAAGAAAGTCCGGGCTCATCTGA
  GTTTTTCCAGGAAGCAGTCTCACATGGGAAGTTTGAGGAACATGAAAACACGAATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230501 representing NM_001286221
  Red=Cloning site Green=Tags(s)
  
  MCIRCLCLIGLQTVSGFFSCQITSHLLSLQVQGEYKCGLLCENTSLLIANVRRKVSGGSATSEDHEFDPS
  ADMLVHDFDDERTLEEEEMMEGETNFSSEIEDLAREGDMPIHELLSLYGYDSTVRLPEEEEEEEEEEEGE
  DDEDADNDDNSGCSGENKEENIKDSSGQEDETQSSNDDPSQSVTSQDAQEIIRPRRCKYFDTNSEIEEES
  EEDEDYIPSEDWKKEIMVGSMFQAEIPVGVCRYKENEKVYENDDQLLWDPECLPEEKVVVFLKDASRRTG
  DEKGVEAIPEGSHIKDNEQALYELVKCSFDTEEALRRLRFNVKAAREELSVWTEEECRNFEQGLKAYGKD
  FHLIQANKVRTRSVGECVAFYYMWKKSERYDFFAQQTRFGKKKYNLHPGVTDYMDRLLDESESAASSRAP
  SPPPTASNSSNSQSEKEDGAVSSRNQNGVSSNGPGEILNKEEVKVEGLHVNGPTGGNKKPLLTDMDTNGY
  EANNLTTDPKLAHMTARNENDFDEKNERPAKRRRINSSGKESPGSSEFFQEAVSHGKFEEHENTND
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286221
• ORF Size: 1668 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001286221.1, NP_001273150.1
• RefSeq Size: 4781
• RefSeq ORF: 1671
• Locus ID: 71148
• MW: 63.4 kDa