Gk (NM_001294140) Mouse Tagged ORF Clone

CAT#: MR230516

Gyk (myc-DDK-tagged) - Mouse glycerol kinase (Gyk), transcript variant 4

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gk
• Synonyms: D930012N15Rik; Gyk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230516 representing NM_001294140
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCGCGAAGAAAGCAGTTCTGGGGCCATTGGTGGGAGCAGTGGACCAGGGTACCAGCTCGACAC
  GTTTTTTGGTTTTCAATTCAAAAACAGCTGAACTTCTTAGTCATCATCAAGTAGAAATAAAACAGGAATT
  CCCAAGAGAAGGATGGGTAGAACAAGACCCGAAGGAAATTCTGCAGTCTGTTTATGAGTGTATAGAGAAA
  ACGTGTGAGAAACTTGGACAGCTCAATATTGATATTTCCAACATCAAAGCCATTGGTGTCAGCAACCAGA
  GGGAAACCACAGTAGTCTGGGACAAGGTCACCGGAGAGCCTCTCTATAATGCCGTGGTGTGGCTTGACCT
  AAGAACCCAGTCTACTGTTGAGAACCTTAGTAAAAGAATTCCAGGAAATAATAACTTTGTCAAGTCCAAG
  ACAGGCCTTCCACTTAGCACGTATTTCAGTGCAGTGAAACTTCGTTGGCTCCTTGACAACGTGAAAAAGG
  TCCAAGAGGCTGTTGAAGAAAATAGAGCTCTTTTTGGGACCATTGATTCATGGCTTATTTGGAGTTTAAC
  AGGAGGAATCCATGGGGGTGTCCACTGTACAGATGTAACAAATGCAAGCAGGACGATGCTTTTTAACATT
  CATTCTTTGGAATGGGATAAAGAGCTCTGCGAATTTTTTGGAATTCCAATGGAAATTCTTCCCAACGTTC
  GGAGTTCTTCTGAGATCTATGGCCTAATGAAAATCTCTCATAGCCTGAAAGCTGGGGCCTTGGAAGGTGT
  ACCAATATCTGGGTGTTTGGGGGACCAGTCTGCTGCTTTGGTGGGACAAATGTGCTTCCAGGATGGACAG
  GCCAAAAACACGTATGGAACAGGGTGCTTCTTATTGTGCAACACGGGCCATAAGTGTGTATTTTCTGAAC
  ATGGCCTCCTGACAACCGTAGCATATAAACTTGGCAGAGACAAACCTGTGTATTATGCGTTGGAAGGTTC
  CGTGGCTATAGCTGGTGCTGTAATCCGCTGGCTAAGAGACAACCTTGGAATTATTAAGTCCTCTGAGGAA
  ATTGAAAAACTTGCTAAGGAAGTAGGTACTTCTTATGGCTGCTACTTCGTTCCAGCATTTTCAGGGTTAT
  ATGCGCCTTATTGGGAGCCCAGTGCAAGAGGGATCATCTGTGGACTCACTCAGTTCACCAATAAATGTCA
  TATCGCTTTTGCTGCACTAGAAGCTGTTTGTTTCCAAACCCGAGAGATTTTGGATGCCATGAATCGCGAC
  TGTGGAATTCCACTCAGTCATTTACAGGTAGATGGAGGAATGACCAGCAATAAAATTCTTATGCAGCTAC
  AAGCAGACATTCTGTATATTCCAGTAGTGAAACCCTCCATGCCTGAAACAACTGCACTAGGCGCTGCCAT
  GGCAGCTGGGGCTGCAGAGGGGGTTGGTGTGTGGAGTCTTGAACCTGAGGATTTGTCAGCTGTCACAATG
  GAGCGGTTTGAACCTCAGATCAATGCTGAAGAAAGCGAAATCCGTTACTCCACATGGAAGAAAGCTGTGA
  TGAAGTCAATTGGTTGGGTTACAACTCAGTCTCCAGAAAGTGGTGACCCTAGTATCTTCTGTAGTCTGCC
  TTTGGGCTTTTTTATAGTGAGTAGCATGGTAATGTTAATCGGAGCAAGGTACATCTCAGGTATCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230516 representing NM_001294140
  Red=Cloning site Green=Tags(s)
  
  MAAAKKAVLGPLVGAVDQGTSSTRFLVFNSKTAELLSHHQVEIKQEFPREGWVEQDPKEILQSVYECIEK
  TCEKLGQLNIDISNIKAIGVSNQRETTVVWDKVTGEPLYNAVVWLDLRTQSTVENLSKRIPGNNNFVKSK
  TGLPLSTYFSAVKLRWLLDNVKKVQEAVEENRALFGTIDSWLIWSLTGGIHGGVHCTDVTNASRTMLFNI
  HSLEWDKELCEFFGIPMEILPNVRSSSEIYGLMKISHSLKAGALEGVPISGCLGDQSAALVGQMCFQDGQ
  AKNTYGTGCFLLCNTGHKCVFSEHGLLTTVAYKLGRDKPVYYALEGSVAIAGAVIRWLRDNLGIIKSSEE
  IEKLAKEVGTSYGCYFVPAFSGLYAPYWEPSARGIICGLTQFTNKCHIAFAALEAVCFQTREILDAMNRD
  CGIPLSHLQVDGGMTSNKILMQLQADILYIPVVKPSMPETTALGAAMAAGAAEGVGVWSLEPEDLSAVTM
  ERFEPQINAEESEIRYSTWKKAVMKSIGWVTTQSPESGDPSIFCSLPLGFFIVSSMVMLIGARYISGIP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001294140
• ORF Size: 1677 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001294140.1, NP_001281069.1
• RefSeq Size: 4462
• RefSeq ORF: 1680
• Locus ID: 14933
• MW: 61.7 kDa