Mcm5 (NM_001302540) Mouse Tagged ORF Clone

CAT#: MR231022

Mcm5 (myc-DDK-tagged) - Mouse minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae) (Mcm5), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mcm5
• Synonyms: AA617332; AI324988; AL033333; Cdc46; Mcmd5; P1-CDC46
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231022 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGGCTTCGACGACCCGGGCATTTTCTACAGCGACAGCTTCGGTGGCGACCCCGGTGCGGAAGAGG
  GCCAGGCCCGCAAGTCGCAACTGCAGAGGCGATTCAAGGAGTTCCTGAGACAGTACCGAGTGGGCACCGA
  TCGCACGGGCTTCACCTTCAAGTACAGAGATGAACTCAAGCGGCATTACAACCTGGGTGAATACTGGATC
  GAGGTGGAGATGGAGGACCTGGCCAGTTTTGACGAGGAACTGGCTGACCACTTGCATAAACAGCCGGCCG
  AGCACTTACAGCTGCTTGAGGAAGCTGCCAAGGAGGTGGCAGATGAGGTGACCCGGCCCCGGCCAGCTGG
  AGATGAGCTGCTCCAAGACATCCAGGTCATGCTCAAGTCAGATGCCAGCCCGTCGAGCATTCGGATTCTG
  AAGTCAGACATGATGTCACACCTGGTGAAGATCCCTGGCATCATCATTTCAGCCTCTGCAGTCCGTGCCA
  AGGCTACTCGTATCTCCATTCAGTGCCGCAGCTGCCACAATACCCTCACCAATATCGCCATGCGCCCAGG
  CCTAGAGGGCTATGCCCTTCCCAGGAAGTGCAATATGGATCAGGCTGGGCGCCCAAAGTGCCCACTGGAT
  CCATACTTCATCATGCCTGACAAGTGCAAGTGTGTGGACTTCCAGACTCTGAAACTGCAGGAGCTGCCTG
  ATGCAGTCCCTCATGGTGAGATGCCCAGGCACATGCAGCTTTATTGTGACAGGTACCTGTGTGACAAGGT
  TGTTCCTGGGAACAGGGTCACCATCATGGGCATTTATTCCATCAAGAAGTTTGGCTTGAACCCCAGCAAG
  GGCCGGGACAGGGTAGGTGTGGGCATCCGGAGCTCGTACATCCGAGTGCTGGGCATCCAGGTGGACACAG
  ATGGCTCTGGCCGAAGCTTTGCTGGGTCTGTCAGCCCACAGGAAGAGGAGGAATTTCGTCGCCTGGCTGC
  CCTCCCCAACATATATGAGCTCATCTCCAAGAGCATTTCCCCCTCCATCTTTGGGGGCATGGATATGAAG
  AAGGCCATTGCCTGCCTGCTTTTTGGGGGTTCCCGGAAGAGGCTCCCAGATGGACTCACTCGCCGAGGTG
  ATATCAACTTGCTGATGTTGGGAGACCCTGGTACAGCCAAGTCTCAGCTTCTGAAGTTTGTGGAGAAGTG
  CTCTCCCATTGGGGTGTACACATCTGGGAAAGGTAGCAGTGCTGCTGGCTTGACTGCCTCAGTGATACGG
  GACCCCTCATCTCGAAACTTCATCATGGAAGGTGGAGCCATGGTTCTGGCCGATGGTGGGGTTGTCTGTA
  TTGATGAGTTTGACAAGATGCGGGAAGATGACCGTGTTGCAATCCATGAGGCTATGGAGCAGCAGACCAT
  CTCCATTGCTAAAGCTGGGATCACTACCACCTTGAACTCTCGCTGCTCTGTTCTGGCTGCAGCCAACTCA
  GTGTTTGGCCGATGGGATGAGACAAAAGGGGAGGACAATATTGACTTCATGCCTACCATCTTGTCCCGAT
  TTGATATGATCTTCATCGTCAAAGATGAGCACAATGAGGAGAGGGACATGATGCTAGCCAAACATGTGAT
  GACTCTGCATGTGAGTGCACTGACACAGACACAGGCTGTGGAGGGTGAGATCGACCTGGCCAAGATGAAG
  AAGTTCATTGCCTACTGCCGAGCGAGGTGTGGACCTCGGCTATCAGCAGAGGCAGCAGAGAAGCTGAAAA
  ACCGCTACATCATCATGCGGAGTGGGGCTCGTCAGCATGAGAGGGACAGTGACCGGCGTTCCAGCATCCC
  CATCACTGTGCGGCAGCTGGAGGCTATTGTGCGCATTGCTGAGGCCCTCAGTAAGATGAAACTGCAGCCC
  TTTGCCACTGAGGCTGATGTAGAGGAGGCATTGAGACTGTTCCAGGTGTCCACACTGGATGCTGCTTTGT
  CTGGCAATCTGTCGGGGGTGGAGGGCTTCACTACCCAGGAGGACCAGGAGATGCTGAGCCGCATTGAGAA
  GCAACTCAAGCGCCGTTTTGCCATTGGCTCTCAGGTGTCTGAACACAGCATTGTCCAGGACTTCACCAAA
  CAGAAATATCCAGAGCACGCTATCCGAAAGGTGCTGCAGCTCATGCTACGCAGGGGTGAGATCCAACACC
  GTATGCAGCGCAAGGTGCTCTATCGCCTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231022 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGFDDPGIFYSDSFGGDPGAEEGQARKSQLQRRFKEFLRQYRVGTDRTGFTFKYRDELKRHYNLGEYWI
  EVEMEDLASFDEELADHLHKQPAEHLQLLEEAAKEVADEVTRPRPAGDELLQDIQVMLKSDASPSSIRIL
  KSDMMSHLVKIPGIIISASAVRAKATRISIQCRSCHNTLTNIAMRPGLEGYALPRKCNMDQAGRPKCPLD
  PYFIMPDKCKCVDFQTLKLQELPDAVPHGEMPRHMQLYCDRYLCDKVVPGNRVTIMGIYSIKKFGLNPSK
  GRDRVGVGIRSSYIRVLGIQVDTDGSGRSFAGSVSPQEEEEFRRLAALPNIYELISKSISPSIFGGMDMK
  KAIACLLFGGSRKRLPDGLTRRGDINLLMLGDPGTAKSQLLKFVEKCSPIGVYTSGKGSSAAGLTASVIR
  DPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANS
  VFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDMMLAKHVMTLHVSALTQTQAVEGEIDLAKMK
  KFIAYCRARCGPRLSAEAAEKLKNRYIIMRSGARQHERDSDRRSSIPITVRQLEAIVRIAEALSKMKLQP
  FATEADVEEALRLFQVSTLDAALSGNLSGVEGFTTQEDQEMLSRIEKQLKRRFAIGSQVSEHSIVQDFTK
  QKYPEHAIRKVLQLMLRRGEIQHRMQRKVLYRLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001302540
• ORF Size: 2205 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001302540.1, NP_001289469.1
• RefSeq Size: 3417
• RefSeq ORF: 2205
• Locus ID: 17218
• MW: 82.4 kDa
• Gene Summary: The protein encoded by this gene is a member of the oligameric minichromosome maintenance protein complex. During DNA replication, the complex loads onto chromatin in early G1 and is converted into an active replicative helicase during S phase. It functions to limit DNA synthesis to once per cell cycle. During embryogenesis, the encoded protein is negatively regulated through expression of paired box protein Pax 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]