Sema3b (NM_001291537) Mouse Tagged ORF Clone

CAT#: MR231055

Sema3b (myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B (Sema3b), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sema3b
• Synonyms: SemA; sema5; Semaa; semaV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231055 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGGGCTGAGGCCGCCGCCATGATCCCAGGCCTGGCCCTTCTCTGGGTAGCAGGGCTAGGGGATA
  CTGCCCCTAACCTTCCCCGCCTTCGGCTCTCCTTTCAAGAATTACAGGCCCGGCATGGTGTCCGAACCTT
  CAGGCTGGAGCGGACCTGCTGTTATGAAGCCTTGCTGGTGGATGAGGAGCGTGGACGCCTGTTTGTGGGT
  GCTGAGAACCACGTGGCTTCCCTCAGCCTGGACAACATCAGCAAGCGAGCCAAGAAGCTGGCCTGGCCCG
  CCCCCGTGGAATGGCGTGAAGAATGCAACTGGGCAGGGAAGGACATTGGTACCGAGTGCATGAACTTCGT
  GAAGCTGCTGCACACCTACAACCACACCCACTTGCTGGCCTGTGGCACAGGGGCTTTCCACCCAACCTGT
  GCCTTTGTGGAGGTGGGCCACCGGCTGGAGGAACCCATGCTTCAACTGGACCGGAGGAAACTTGAGGACG
  GCAAGGGGAAGACTCCTTATGACCCAAGGCATCGGGCTGCCTCGGTGCTGGTGGGGGAAGAACTGTATTC
  TGGGGTGACAGCAGACCTTATGGGCCGGGACTTTACCATCTTTCGAAGCCTTGGTCAGAATCCGAGTCTC
  CGAACAGAGCCCCATGATTCCCGCTGGCTCAATGAACCCAAGTTTGTCAAGGTCTTTTGGATCCCAGAGA
  GTGAGAACCCTGATGACGATAAAATCTATTTCTTCTTCCGCGAGTCCGCTGTGGAAGCAGCACCAGCAAT
  GGGGCGCATGTCTGTGTCTCGTGTTGGCCAGATCTGCAGGAATGACCTGGGTGGCCAGCGGAGCTTGGTC
  AACAAATGGACCACATTTCTGAAGGCGCGGCTTGTGTGCTCAGTACCTGGAGTTGAGGGTGACACCCACT
  TTGACCAACTTCAGGATGTTTTCCTTCTGTCCTCCCGAGACCGCCAGACACCTCTTCTCTATGCTGTCTT
  CTCCACCTCCAGTGGTGTCTTCCAGGGCTCTGCTGTGTGCGTGTACAGCATGAACGATGTGCGCCGAGCC
  TTCTTGGGACCTTTTGCTCACAAAGAGGGGCCTACACACCAGTGGGTGTCCTACCAGGGTCGTGTCCCCT
  ACCCAAGACCTGGCATGTGCCCCAGCAAGACCTTTGGCACCTTCAGCTCCACCAAGGACTTCCCAGATGA
  CGTTATCCAGTTTGCTCGGAACCACCCTCTCATGTACAACCCAGTCCTGCCCATGGGGGGGCGCCCTCTC
  TTCCTACAAGTGGGAGCTGGGTACACCTTCACCCAAATCGCCGCAGACCGAGTAGCAGCTGCCGATGGAC
  ACTACGATGTTCTCTTCATTGGTACAGATGTGGGCACAGTGCTGAAAGTGATCTCAGTCCCCAAAGGCAG
  CCGACCTAATTCTGAAGGACTTCTCCTGGAAGAGCTGCAGGTGTTCGAGGACTCTGCCGCTATCACCAGC
  ATGCAAATCTCCTCTAAAAGGCAACAACTCTACATAGCATCGCGCAGCGCAGTGGCCCAGATTGCTTTGC
  ATCGCTGCACTGCCCTAGGCCGCGCCTGCGCAGAATGCTGCTTGGCCCGTGATCCTTACTGCGCCTGGGA
  TGGATCAGCTTGCACACGCTTCCAGCCTACGGCCAAGAGACGGTTCCGGAGGCAAGACATAAGGAATGGC
  GACCCCAGCACCCTATGCTCTGGAGACTCTTCTCACTCTGTGCTGCTGGAGAAGAAGGTGTTGGGTGTGG
  AGAGCGGCAGCGCGTTTCTGGAGTGTGAGCCCCGCTCGCTCCAGGCGCATGTGCAGTGGACCTTCCAAGG
  TGCAGGGGAGGCAGCTCACACCCAGGTGCTGGCTGAGGAGAGAGTAGAGCGCACTGCGCGGGGGCTGCTG
  TTGCGGGGGCTGCGGCGCCAGGACTCTGGCGTGTATCTTTGCGTCGCGGTTGAACAAGGCTTTTCACAAC
  CACTGCGTCGCCTGGTGCTGCATGTGTTGAGTGCGGCGCAGGCTGAACGACTGGCACGGGCAGAGGAAGC
  AGCCGCTCCTGCACCTCCTGGCCCTAAACTCTGGTACCGGGACTTTCTGCAGTTGGTGGAGCCAGGCGGT
  GGCGGAGGTGCAAACTCCCTGCGAATGTGCCGCCCGCAGCCCGGGCACCACTCTGTGGCAGCAGATTCAC
  GTCGTAAGGGTCGCAACAGACGGATGCATGTCTCTGAGCTCCGTGCTGAGCGTGGACCACGTAGTGCAGC
  TCACTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231055 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGRAEAAAMIPGLALLWVAGLGDTAPNLPRLRLSFQELQARHGVRTFRLERTCCYEALLVDEERGRLFVG
  AENHVASLSLDNISKRAKKLAWPAPVEWREECNWAGKDIGTECMNFVKLLHTYNHTHLLACGTGAFHPTC
  AFVEVGHRLEEPMLQLDRRKLEDGKGKTPYDPRHRAASVLVGEELYSGVTADLMGRDFTIFRSLGQNPSL
  RTEPHDSRWLNEPKFVKVFWIPESENPDDDKIYFFFRESAVEAAPAMGRMSVSRVGQICRNDLGGQRSLV
  NKWTTFLKARLVCSVPGVEGDTHFDQLQDVFLLSSRDRQTPLLYAVFSTSSGVFQGSAVCVYSMNDVRRA
  FLGPFAHKEGPTHQWVSYQGRVPYPRPGMCPSKTFGTFSSTKDFPDDVIQFARNHPLMYNPVLPMGGRPL
  FLQVGAGYTFTQIAADRVAAADGHYDVLFIGTDVGTVLKVISVPKGSRPNSEGLLLEELQVFEDSAAITS
  MQISSKRQQLYIASRSAVAQIALHRCTALGRACAECCLARDPYCAWDGSACTRFQPTAKRRFRRQDIRNG
  DPSTLCSGDSSHSVLLEKKVLGVESGSAFLECEPRSLQAHVQWTFQGAGEAAHTQVLAEERVERTARGLL
  LRGLRRQDSGVYLCVAVEQGFSQPLRRLVLHVLSAAQAERLARAEEAAAPAPPGPKLWYRDFLQLVEPGG
  GGGANSLRMCRPQPGHHSVAADSRRKGRNRRMHVSELRAERGPRSAAHW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291537
• ORF Size: 2247 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001291537.1, NP_001278466.1
• RefSeq Size: 4010
• RefSeq ORF: 2250
• Locus ID: 20347
• MW: 83.1 kDa
• Gene Summary: This gene encodes a secreted protein that belongs to the class 3 semaphorin/collapsin family. Members of this family play a role in growth cone guidance during neurogenesis. The encoded protein inhibits axonal extension. This protein is thought to be an osteoblast protein that regulates bone mass and affects skeletal homeostasis. A similar gene in humans functions as a tumor suppressor gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]