Dnmt3b (NM_001271745) Mouse Tagged ORF Clone

CAT#: MR231240

Dnmt3b (myc-DDK-tagged) - Mouse DNA methyltransferase 3B (Dnmt3b), transcript variant 7

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dnmt3b
• Synonyms: MmuIIIB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231240 representing NM_001271745
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGAGACAGCAGACATCTGAATGAAGAAGAGGGTGCCAGCGGGTATGAGGAGTGCATTATCGTTA
  ATGGGAACTTCAGTGACCAGTCCTCAGACACGAAGGATGCTCCCTCACCCCCAGTCTTGGAGGCAATCTG
  CACAGAGCCAGTCTGCACACCAGAGACCAGAGGCCGCAGGTCAAGCTCCCGGCTGTCTAAGAGGGAGGTC
  TCCAGCCTTCTGAATTACACGCAGGACATGACAGGAGATGGAGACAGAGATGATGAAGTAGATGATGGGA
  ATGGCTCTGATATTCTAATGCCAAAGCTCACCCGTGAGACCAAGGACACCAGGACGCGCTCTGAAAGCCC
  GGCTGTCCGAACCCGACATAGCAATGGGACCTCCAGCTTGGAGAGGCAAAGAGCCTCCCCCAGAATCACC
  CGAGGTCGGCAGGGCCGCCACCATGTGCAGGAGTACCCTGTGGAGTTTCCGGCTACCAGGTCTCGGAGAC
  GTCGAGCATCATCTTCAGCAAGCACGCCATGGTCATCCCCTGCCAGCGTCGACTTCATGGAAGAAGTGAC
  ACCTAAGAGCGTCAGTACCCCATCAGTTGACTTGAGCCAGGATGGAGATCAGGAGGGTATGGATACCACA
  CAGGTGGATGCAGAGAGCAGAGATGGAGACAGCACAGAGTATCAGGATGATAAAGAGTTTGGAATAGGTG
  ACCTCGTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCTGCCATGGTGGTGTCCTGGAAAGCCACCTC
  CAAGCGACAGGCCATGCCCGGAATGCGCTGGGTACAGTGGTTTGGTGATGGCAAGTTTTCTGAGATCTCT
  GCTGACAAACTGGTGGCTCTGGGGCTGTTCAGCCAGCACTTTAATCTGGCTACCTTCAATAAGCTGGTTT
  CTTATAGGAAGGCCATGTACCACACTCTGGAGAAAGCCAGGGTTCGAGCTGGCAAGACCTTCTCCAGCAG
  TCCTGGAGAGTCACTGGAGGACCAGCTGAAGCCCATGCTGGAGTGGGCCCACGGTGGCTTCAAGCCTACT
  GGGATCGAGGGCCTCAAACCCAACAAGAAGCAACCAGAGAACAAAAGTCGAAGACGCACAACCAATGACT
  CTGCTGCTTCTGAGTCCCCCCCACCCAAGCGCCTCAAGACAAATAGCTATGGCGGGAAGGACCGAGGGGA
  GGATGAGGAGAGCCGAGAACGGATGGCTTCTGAAGTCACCAACAACAAGGGCAATCTGGAAGACCGCTGT
  TTGTCCTGTGGAAAGAAGAACCCTGTGTCCTTCCACCCCCTCTTTGAGGGTGGGCTCTGTCAGAGTTGCC
  GGGATCGCTTCCTAGAGCTCTTCTACATGTATGATGAGGACGGCTATCAGTCCTACTGCACCGTGTGCTG
  TGAGGGCCGTGAACTGCTGCTGTGCAGTAACACAAGCTGCTGCAGATGCTTCTGTGTGGAGTGTCTGGAG
  GTGCTGGTGGGCGCAGGCACAGCTGAGGATGCCAAGCTGCAGGAACCCTGGAGCTGCTATATGTGCCTCC
  CTCAGCGCTGCCATGGGGTCCTCCGACGCAGGAAAGATTGGAACATGCGCCTGCAAGACTTCTTCACTAC
  TGATCCTGACCTGGAAGAATTTGAGCCACCCAAGTTGTACCCAGCAATTCCTGCAGCCAAAAGGAGGCCC
  ATTAGAGTCCTGTCTCTGTTTGATGGAATTGCAACGGGGTACTTGGTGCTCAAGGAGTTGGGTATTAAAG
  TGGAAAAGTACATTGCCTCCGAAGTCTGTGCAGAGTCCATCGCTGTGGGAACTGTTAAGCATGAAGGCCA
  GATCAAATATGTCAATGACGTCCGGAAAATCACCAAGAAAAATATTGAAGAGTGGGGCCCGTTCGACTTG
  GTGATTGGTGGAAGCCCATGCAATGATCTCTCTAACGTCAATCCTGCCCGCAAAGGTTTATATGAGGGCA
  CAGGAAGGCTCTTCTTCGAGTTTTACCACTTGCTGAATTATACCCGCCCCAAGGAGGGCGACAACCGTCC
  ATTCTTCTGGATGTTCGAGAATGTTGTGGCCATGAAAGTGAATGACAAGAAAGACATCTCAAGATTCCTG
  GCATGTAACCCAGTGATGATCGATGCCATCAAGGTGTCTGCTGCTCACAGGGCCCGGTACTTCTGGGGTA
  ACCTACCCGGAATGAACAGGCCCGTGATGGCTTCAAAGAATGATAAGCTCGAGCTGCAGGACTGCCTGGA
  GTTCAGTAGGACAGCAAAGTTAAAGAAAGTGCAGACAATAACCACCAAGTCGAACTCCATCAGACAGGGC
  AAAAACCAGCTTTTCCCTGTAGTCATGAATGGCAAGGACGACGTTTTGTGGTGCACTGAGCTCGAAAGGA
  TCTTCGGCTTCCCTGCTCACTACACGGACGTGTCCAACATGGGCCGCGGCGCCCGTCAGAAGCTGCTGGG
  CAGGTCCTGGAGTGTACCGGTCATCAGACACCTGTTTGCCCCCTTGAAGGACTACTTTGCCTGTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231240 representing NM_001271745
  Red=Cloning site Green=Tags(s)
  
  MKGDSRHLNEEEGASGYEECIIVNGNFSDQSSDTKDAPSPPVLEAICTEPVCTPETRGRRSSSRLSKREV
  SSLLNYTQDMTGDGDRDDEVDDGNGSDILMPKLTRETKDTRTRSESPAVRTRHSNGTSSLERQRASPRIT
  RGRQGRHHVQEYPVEFPATRSRRRRASSSASTPWSSPASVDFMEEVTPKSVSTPSVDLSQDGDQEGMDTT
  QVDAESRDGDSTEYQDDKEFGIGDLVWGKIKGFSWWPAMVVSWKATSKRQAMPGMRWVQWFGDGKFSEIS
  ADKLVALGLFSQHFNLATFNKLVSYRKAMYHTLEKARVRAGKTFSSSPGESLEDQLKPMLEWAHGGFKPT
  GIEGLKPNKKQPENKSRRRTTNDSAASESPPPKRLKTNSYGGKDRGEDEESRERMASEVTNNKGNLEDRC
  LSCGKKNPVSFHPLFEGGLCQSCRDRFLELFYMYDEDGYQSYCTVCCEGRELLLCSNTSCCRCFCVECLE
  VLVGAGTAEDAKLQEPWSCYMCLPQRCHGVLRRRKDWNMRLQDFFTTDPDLEEFEPPKLYPAIPAAKRRP
  IRVLSLFDGIATGYLVLKELGIKVEKYIASEVCAESIAVGTVKHEGQIKYVNDVRKITKKNIEEWGPFDL
  VIGGSPCNDLSNVNPARKGLYEGTGRLFFEFYHLLNYTRPKEGDNRPFFWMFENVVAMKVNDKKDISRFL
  ACNPVMIDAIKVSAAHRARYFWGNLPGMNRPVMASKNDKLELQDCLEFSRTAKLKKVQTITTKSNSIRQG
  KNQLFPVVMNGKDDVLWCTELERIFGFPAHYTDVSNMGRGARQKLLGRSWSVPVIRHLFAPLKDYFACE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271745
• ORF Size: 2520 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001271745.1, NP_001258674.1
• RefSeq Size: 4166
• RefSeq ORF: 2520
• Locus ID: 13436
• MW: 94.8 kDa
• Gene Summary: This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene results in severe developmental defects and loss of viability. Mutation of the related gene in humans causes immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. There is a pseudogene for this gene located adjacent to this gene in the same region of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012]