Adam17 (NM_001277266) Mouse Tagged ORF Clone

CAT#: MR231248

Adam17 (myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 17 (Adam17), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adam17
• Synonyms: CD156b; Tace
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231248 representing NM_001277266
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGGCGTCTCCTCATCCTGACCACTTTGGTGCCTTTCGTCCTGGCACCCCGACCTCCGGAGGAAG
  CAGGCTCTGGCTCCCATCCGCGACTTGAGAAGCTTGATTCTTTGCTCTCAGACTACGACATCCTCTCCTT
  AGCTAATATTCAGCAGCACTCCATAAGGAAAAGGGATCTACAGTCTGCGACACACTTAGAAACATTACTA
  ACTTTTTCAGCTTTGAAAAGACATTTTAAATTATACTTGACATCAAGTACCGAACGCTTTTCACAAAACT
  TGAGAGTCGTGGTGGTGGACGGGAAAGAAGAAAGCGAGTACAGCGTGAAGTGGCAGAACTTCTTCAGTGG
  TCACGTGGTTGGTGAGCCTGACTCTAGGGTTCTAGCCCACATAGGAGATGATGATGTTACAGTGAGAATC
  AACACAGATGGGGCAGAATATAACGTAGAGCCACTTTGGAGGTTTGTCAATGATACTAAAGATAAACGAA
  TGCTGGTGTATAAGTCTGAAGATATCAAGGATTTTTCACGTTTGCAGTCTCCAAAAGTATGTGGTTATTT
  AAATGCAGATAGTGAAGAGCTGCTTCCAAAAGGGCTCATAGACAGAGAGCCATCTGAAGAGTTTGTTCGT
  CGAGTGAAGAGACGAGCTGAACCTAACCCCTTGAAGAATACTTGTAAATTACTGGTGGTAGCAGATCATC
  GATTTTATAAATACATGGGCCGTGGAGAAGAGAGCACCACTACAAATTACTTAGGCTACATTTCAGGCAC
  TCGGGACAGAGTGACGACCGACTGCTTTTTAGAGTTTTTGATAGAGCTAATTGACCGAGTTGATGACATA
  TACCGGAACACGTCGTGGGATAATGCAGGGTTTAAAGGGTATGGAGTGCAGATAGAGCAGATTCGAATTC
  TCAAGTCTCCACAAGAGGTAAAACCTGGTGAAAGACACTTCAATATGGCAAAAAGTTTCCCAAACGAAGA
  GAAGGATGCTTGGGATGTGAAGATGCTATTAGAGCAATTTAGCTTTGATATAGCTGAAGAAGCATCTAAA
  GTCTGCCTGGCTCATCTTTTCACGTACCAGGATTTTGATATGGGAACTCTTGGATTAGCTTACGTTGGTT
  CTCCCAGAGCAAACAGTCATGGAGGGGTTTGTCCAAAAGCTTATTACAACCCAACTGTGAAGAAAAACAT
  CTATTTAAATAGTGGTCTGACTAGTACTAAAAATTATGGCAAAACTATTCTCACAAAGGAAGCTGACCTG
  GTTACAACTCATGAATTGGGACATAATTTTGGAGCAGAACATGACCCTGATGGGCTAGCAGAATGTGCCC
  CAAATGAGGACCAAGGAGGAAAGTATGTCATGTATCCCATAGCTGTGAGCGGTGACCACGAGAATAATAA
  GATGTTTTCAAACTGCAGTAAACAGTCCATCTACAAGACCATAGAAAGTAAGGCTCAAGAGTGCTTCCAG
  GAGCGCAGCAACAAGGTGTGTGGCAACTCCAGGGTGGATGAAGGAGAGGAGTGTGACCCGGGTATTATGT
  ACCTGAACAACGACACCTGCTGCAATAGTGACTGCACACTGAAGCCGGGTGTGCAGTGCAGTGATAGGAA
  CAGTCCTTGCTGTAAAAACTGTCAGTTTGAGACGGCGCAGAAGAAGTGCCAGGAGGCTATTAATGCTACA
  TGCAAAGGAGTGTCTTACTGCACAGGGAATAGCAGTGAGTGCCCCCCACCCGGAGATGCTGAAGATGACA
  CTGTGTGCTTGGACCTTGGCAAGTGCAAGGCTGGGAAATGCATCCCTTTCTGCAAGAGGGAGCAGGAGCT
  GGAGTCCTGCGCATGCGTTGACACTGACAACTCGTGCAAGGTGTGCTGCAGGAACCTTTCTGGCCCGTGT
  GTGCCGTACGTCGATGCAGAGCAAAAGAACTTGTTTTTGAGGAAAGGGAAGCCATGTACAGTAGGGTTTT
  GCGACATGAATGGCAAATGTGAGAAACGAGTACAGGACGTAATTGAGCGATTTTGGGATTTCATTGACCA
  GCTGAGCATCAACACTTTTGGGAAGTTTCTGGCAGATAACATCGTTGGGTCTGTTCTGGTTTTCTCCTTG
  ATATTTTGGATTCCTTTCAGCATTCTTGTCCACTGTGTGGATAAGAAACTGGACAAGCAGTATGAATCCC
  TGTCTCTGTTTCATCACAGTAACATTGAGATGCTGAGCAGCATGGACTCAGCATCTGTTCGCATCATCAA
  GCCCTTTCCTGCACCCCAGACTCCAGGTCGTCTGCAGGCCCTGCAGCCAGCTGCCATGATGCCGCCAGTA
  CCTGCAGCTCCAAAACTGGACCACCAGAGGATGGACACCATCCAGGAAGACCCCAGCACAGACTCACATG
  CAGATGATGACGGTTTTGAGAAGGACCCCTTCCCCAACAGCAGCACAGCTGCCAAGTCCTTTGAGGATCT
  CACAGACCACCCAGTCACCAGGAGCGAAAAGGCGGCCTCATTCAAGCTGCAGCGTCAGAGCCGAGTTGAC
  AGCAAAGAGACAGAGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231248 representing NM_001277266
  Red=Cloning site Green=Tags(s)
  
  MRRRLLILTTLVPFVLAPRPPEEAGSGSHPRLEKLDSLLSDYDILSLANIQQHSIRKRDLQSATHLETLL
  TFSALKRHFKLYLTSSTERFSQNLRVVVVDGKEESEYSVKWQNFFSGHVVGEPDSRVLAHIGDDDVTVRI
  NTDGAEYNVEPLWRFVNDTKDKRMLVYKSEDIKDFSRLQSPKVCGYLNADSEELLPKGLIDREPSEEFVR
  RVKRRAEPNPLKNTCKLLVVADHRFYKYMGRGEESTTTNYLGYISGTRDRVTTDCFLEFLIELIDRVDDI
  YRNTSWDNAGFKGYGVQIEQIRILKSPQEVKPGERHFNMAKSFPNEEKDAWDVKMLLEQFSFDIAEEASK
  VCLAHLFTYQDFDMGTLGLAYVGSPRANSHGGVCPKAYYNPTVKKNIYLNSGLTSTKNYGKTILTKEADL
  VTTHELGHNFGAEHDPDGLAECAPNEDQGGKYVMYPIAVSGDHENNKMFSNCSKQSIYKTIESKAQECFQ
  ERSNKVCGNSRVDEGEECDPGIMYLNNDTCCNSDCTLKPGVQCSDRNSPCCKNCQFETAQKKCQEAINAT
  CKGVSYCTGNSSECPPPGDAEDDTVCLDLGKCKAGKCIPFCKREQELESCACVDTDNSCKVCCRNLSGPC
  VPYVDAEQKNLFLRKGKPCTVGFCDMNGKCEKRVQDVIERFWDFIDQLSINTFGKFLADNIVGSVLVFSL
  IFWIPFSILVHCVDKKLDKQYESLSLFHHSNIEMLSSMDSASVRIIKPFPAPQTPGRLQALQPAAMMPPV
  PAAPKLDHQRMDTIQEDPSTDSHADDDGFEKDPFPNSSTAAKSFEDLTDHPVTRSEKAASFKLQRQSRVD
  SKETEC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001277266
• ORF Size: 2538 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001277266.1, NP_001264195.1
• RefSeq Size: 4508
• RefSeq ORF: 2541
• Locus ID: 11491
• MW: 95.7 kDa
• Gene Summary: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature enzyme that is involved in the proteolytic release of membrane-bound proteins in a process called ectodomain shedding. Mice lacking the encoded protein die in utero or fail to survive beyond one week of age. Alternative splicing results in multiple transcript variants encoding different isoforms, some of which may undergo similar processing. [provided by RefSeq, May 2016]