Trpc6 (NM_001282086) Mouse Tagged ORF Clone

CAT#: MR231259

Trpc6 (myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 6 (Trpc6), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trpc6
• Synonyms: AV025995; LLHWJM002; LLHWJM003; LLHWJM004; mtrp6; TRP-6; Trrp6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231259 representing NM_001282086
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCAGAGCCCGAGGTTCGTGACCCGGAGGGGCGGCTCTCTAAAGGCTGCCCCTGGAGCCGGCACCC
  GGCGCAACGAGAGCCAGGACTATTTGCTGATGGACGAGCTGGGAGACGACGGCTACCCGCAGCTCCCGCT
  GCCACCGTATGGCTACTACCCCAGCTTCCGGGGTAATGAAAACAGACTGACTCACCGGCGGCAGACGATT
  CTTCGTGAGAAGGGAAGAAGGTTAGCTAATCGAGGACCAGCATACATGTTTAATGATCATTCAACAAGCC
  TGTCTATTGAGGAAGAACGCTTTCTAGATGCAGCTGAATATGGCAACATCCCAGTGGTGCGGAAGATGCT
  AGAAGAGTGTCATTCCCTCAATGTTAACTGTGTGGATTACATGGGCCAGAATGCCCTACAGCTGGCTGTG
  GCCAATGAGCACTTGGAAATCACAGAGCTGCTACTCAAGAAGGAAAACTTGTCTCGAGTTGGGGATGCTT
  TACTTTTAGCCATTAGTAAAGGTTATGTACGGATTGTGGAGGCAATCCTCAACCATCCAGCTTTTGCTGA
  AGGCAAAAGGTTAGCGACAAGCCCCAGCCAGTCTGAACTTCAGCAAGATGACTTTTATGCCTATGATGAA
  GATGGGACGCGGTTCTCCCATGATGTGACCCCAATCATTCTCGCTGCACATTGCCAGGAATATGAAATTG
  TGCATACCCTCCTGAGAAAGGGTGCCCGGATTGAGCGGCCTCATGATTACTTCTGCAAGTGTACAGAATG
  CAGCCAGAAGCAGAAGCATGATTCCTTCAGCCACTCTAGATCCAGGATCAATGCATACAAAGGTCTGGCA
  AGTCCAGCATACCTGTCATTGTCCAGTGAAGATCCAGTCATGACTGCTTTAGAACTTAGCAATGAGCTGG
  CAGTGCTTGCCAACATTGAGAAAGAGTTCAAGAATGACTACAGGAAGCTGTCTATGCAGTGCAAGGATTT
  CGTTGTTGGTCTCTTGGACCTCTGCAGAAACACAGAGGAAGTGGAGGCCATCCTGAATGGGGATGCAGAG
  ACTCGCCAGCCCGGGGACTTCGGCCGTCCAAATCTCAGCCGTTTAAAACTTGCTATTAAGTATGAAGTAA
  AAAAATTTGTGGCTCATCCAAACTGTCAGCAACAGCTCCTGTCCATATGGTATGAGAACCTCTCTGGTTT
  ACGGCAGCAGACCATGGCAGTGAAGTTCCTCGTGGTCCTTGCTGTTGCCATTGGATTGCCCTTCCTGGCT
  CTCATATACTGGTGTGCTCCTTGCAGCAAGATGGGGAAGATATTGCGAGGACCGTTCATGAAGTTTGTAG
  CACACGCAGCCTCCTTCACCATTTTCCTGGGGCTGCTCGTCATGAATGCAGCTGACAGATTTGAAGGCAC
  CAAGCTCCTCCCTAATGAAACCAGCACAGATAATGCAAGGCAGCTGTTCAGGATGAAAACATCCTGTTTC
  TCATGGATGGAGATGCTCATTATATCCTGGGTAATAGCCAGGATAAAGTGGGACCCTACTGATCCTCAGA
  TCATCTCTGAAGGTCTTTATGCAATCGCTGTGGTTTTAAGTTTCTCCAGAATAGCTTACATTTTACCAGC
  AAATGAAAGCTTTGGACCTCTGCAGATTTCACTTGGAAGAACAGTGAAAGATATCTTCAAATTCATGGTC
  ATATTCATCATGGTGTTTGTAGCCTTTATGATTGGAATGTTCAACCTTTACTCCTACTACATTGGCGCAA
  AACAGAATGAAGCATTCACAACAGTTGAGGAAAGTTTTAAGACACTGTTCTGGGCTATCTTTGGTCTTTC
  TGAAGTGAAGTCAGTGGTCATTAACTACAATCACAAGTTCATTGAAAACATCGGCTACGTTCTGTATGGT
  GTCTATAATGTCACAATGGTCATTGTTTTGCTAAATATGTTAATTGCGATGATCAATAGTTCATTCCAGG
  AAATTGAGGATGATGCGGACGTGGAGTGGAAGTTTGCAAGGGCCAAATTGTGGTTTTCCTACTTTGAGGA
  GGGGAGAACACTTCCTGTCCCCTTCAATCTTGTACCAAGTCCAAAATCCTTGCTTTATCTCCTATTGAAA
  TTTAAGAAATGGATGTGTGAGCTCATCCAGGGTCAAAAGCAAGGCTTCCAAGAAGATGCAGAGATGAACA
  AGAGAAATGAAGAAAAGAAATTTGGAATTTCAGGAAGTCACGAAGACCTTTCAAAATTTTCACTTGACAA
  AAATCAGTTGGCACACAACAAACAATCAAGTACAAGGAGCTCAGAAGATTATCATTTAAATAGTTTCAGT
  AACCCTCCAAGACAATATCAGAAAATCATGAAGAGACTCATTAAAAGATATGTATTGCAGGCCCAGATTG
  ATAAGGAGAGCGATGAGGTGAATGAAGGGGAATTGAAGGAAATTAAGCAAGACATCTCAAGTCTCCGTTA
  TGAACTCCTTGAAGAGAAATCACAGAACACAGAAGACCTAGCAGAGCTCATTAGAAAACTCGGGGAGAGA
  CTGTCGTTAGAGCCAAAGCTGGAGGAAAGCCGCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231259 representing NM_001282086
  Red=Cloning site Green=Tags(s)
  
  MSQSPRFVTRRGGSLKAAPGAGTRRNESQDYLLMDELGDDGYPQLPLPPYGYYPSFRGNENRLTHRRQTI
  LREKGRRLANRGPAYMFNDHSTSLSIEEERFLDAAEYGNIPVVRKMLEECHSLNVNCVDYMGQNALQLAV
  ANEHLEITELLLKKENLSRVGDALLLAISKGYVRIVEAILNHPAFAEGKRLATSPSQSELQQDDFYAYDE
  DGTRFSHDVTPIILAAHCQEYEIVHTLLRKGARIERPHDYFCKCTECSQKQKHDSFSHSRSRINAYKGLA
  SPAYLSLSSEDPVMTALELSNELAVLANIEKEFKNDYRKLSMQCKDFVVGLLDLCRNTEEVEAILNGDAE
  TRQPGDFGRPNLSRLKLAIKYEVKKFVAHPNCQQQLLSIWYENLSGLRQQTMAVKFLVVLAVAIGLPFLA
  LIYWCAPCSKMGKILRGPFMKFVAHAASFTIFLGLLVMNAADRFEGTKLLPNETSTDNARQLFRMKTSCF
  SWMEMLIISWVIARIKWDPTDPQIISEGLYAIAVVLSFSRIAYILPANESFGPLQISLGRTVKDIFKFMV
  IFIMVFVAFMIGMFNLYSYYIGAKQNEAFTTVEESFKTLFWAIFGLSEVKSVVINYNHKFIENIGYVLYG
  VYNVTMVIVLLNMLIAMINSSFQEIEDDADVEWKFARAKLWFSYFEEGRTLPVPFNLVPSPKSLLYLLLK
  FKKWMCELIQGQKQGFQEDAEMNKRNEEKKFGISGSHEDLSKFSLDKNQLAHNKQSSTRSSEDYHLNSFS
  NPPRQYQKIMKRLIKRYVLQAQIDKESDEVNEGELKEIKQDISSLRYELLEEKSQNTEDLAELIRKLGER
  LSLEPKLEESRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282086
• ORF Size: 2556 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001282086.1, NP_001269015.1
• RefSeq Size: 3025
• RefSeq ORF: 2559
• Locus ID: 22068
• MW: 98.1 kDa
• Gene Summary: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion. [UniProtKB/Swiss-Prot Function]