Ano5 (NM_001271879) Mouse Tagged ORF Clone

CAT#: MR231263

Ano5 (myc-DDK-tagged) - Mouse anoctamin 5 (Ano5), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ano5
• Synonyms: 9330162L24; Gdd1; Tmem16e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231263 representing NM_001271879
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGAGCAGGAAGGCTTAACAGCCAAAGAAATAGACTATGCTTTCCAACAGAATGAGAACCTGGGCT
  CCAAAGAGACCAGCTTTCTCATCCCTGAAGACTTACAGTCACCGTTCCAGAGAAGTGAGCACAGCAAAGA
  CTCGGTCTTCTTCAGAGATGGAATCCGGCAGATCGACTTTGTCCTGTCCTACGTGGAGGATTTGAAGAAA
  GATGGAGAGCTGAAGGCAGAAAGAAGACGAGAGTTTGAACAAAATCTCAGAAAAACAGGTCTTGACTTGG
  AAACAGAAGACAAATTGAACTCTGAAGATGGGAAAACCTATTTTGTGAAGATTCACGCCCCATGGGAAGT
  CCTGGTCACTTACGCTGAAGTGCTGGGAATCAAGATGCCTATTAAGTTGAGTGACATCCCACGGCCAAAG
  TACCCACCCCTGTCTTACATGCTTGGGGCGGTGAAGCTTCCATCCTCTGTGAAGTATCCTACTCCTGAGT
  ATTTCACTGCCCAGTTCAGCAGACACCGTCAAGAACTCTTCCTCATTGAAGATGAGGCCACTTTCTTTCC
  ATCCTCAACAAGGAACAGAATTGTTTACTATATTCTGTCACGATGCCCTTTTGGAGTGGAAGAGGGGAAG
  AAAAAGATTGGGATCGAGAGACTGCTGAACTCGAACACTTATTTATCTGCCTACCCGCTCCATGATGGCC
  AGTATTGGAAACCATCAAAAACTACTCGCCCCAATGAAAGGTACAACCTTTGCAAGAACTGGGCTCGATT
  TTCCTACTTTTACAAGGAGCAACCTTTTCATTTGATTCGGAATTATTTTGGAGAAAAAATCGGTATCTAT
  TTTGTATTTCTCGGGTATTACACAGAAATGCTGTTGTTTGCGGCTCTGGTCGGCTTAGCCTGCTTCATCT
  ATGGCTTGTTATCAATGGAAAATAACCGAACTAGCACTGAAATCTGTGACCCTGACATCGGAGGTCAGAT
  GATCATGTGCCCACTTTGCGATGAAGTGTGTGATTACTGGAGACTGAACACCACGTGTTTGCATTCAAAG
  TTCTCCCATCTGTTTGATAACGAGTCGACTGTGTTCTTTGCGCTCTTCATGGGGATCTGGGTCACATTGT
  TTCTGGAGTTTTGGAAGCAGCGACAAGCCAGGCTTGAGTACGAATGGGATCTGGTGGACTTTGAAGAAGA
  ACAGCAGCAGCTCCAGCTTCGGCCTGAATTTGAAGCTATGTGTAAACACAAGAAGATGAATCCTGTGACA
  AAGGAAATGGAGCCACACATGCCTCTATGCCATCGCATCCCATGGTACTTTGTGTCAGGGACCACAGTGA
  CCTTTGGGATGGCTCTCCTCCTCAGCAGCATGGTGTCCATCCTCATATACCGCCTGTCAGTCTTTGCCAC
  TTTTGCCAGCTTCATGGAAAGCGAAGCCACCCTGCAGAGCGTGAAGAGCTTCTTTACTCCACAGCTGGCT
  ACGGCTCTCTCTGGATCATGCCTGAACTGCATTGTCATTTTGATACTGAATTTTTTTTATGAGAAGATAT
  CTGCCTGGATTACGAAAATGGGGAAGTTCGTGGGATATCCTGGGAGTTACACATATATGTTCAACATATG
  GAGAAGCGAAGAGTGTGGTCCTGCAGGCTGTCTGATAGAGCTGACCACCCAGCTAACCATCATTATGATT
  GGAAAACAGATTTTTGGAAACATCCATGAAGCCTTTCAACCCTTGATTTTTAATTGGTGGAGACGCCGAA
  GAGCCCGAACCCACTCTGAGAAACTGTACAGTCGTTGGGAGCAAGATCATGACCTTCAGGTTTATGGACA
  TCGTGGGCTGTTTTATGAGTACTTGGAAACAGTTATCCAGTTTGGGTTTGCCACGCTGTTTGTGGCCTCT
  TTTCCTCTGGCACCCCTGTTTGCTCTCATGAACAACATCATGGGAATCCGAGTGGATGCCTGGAAGCTGA
  CAACTCAGTACAGGAGACCTGTCGCAGCGAAAGCCCACTCCATAGGTGTCTGGCAAGACATTCTTTTTGG
  AATGGCCATCGTTTCTGTTGCAACTAATGCCTTCATTGTCTCATTTACATCTGACATCATCCCCCGGTTA
  GTTTACTTCTATGCCTACTCAACGAATTCCACAGAGCCCTTGAGTGGATACGTCAATAACAGCCTGTCAG
  TGTTCCTGATAGCTGACTTTCCCAACCACACAGTGCCCATGGAGAAAAAAGACTTCGTCACCTGCAGGTA
  CAGAGATTATAGGTACCCTCCTGATCATGAGGATAAGTATAGCCATAACATGCAGTTCTGGCATGTTCTT
  GCTGCCAAGATGACCTTCATCATAGTGATGGAACACGTCGTGTTTCTGTTTAAATTTTTATTAGCCTGGC
  TGATACCAGATGTTCCTAAAGATGTTGTGGAGAAAATCAAACGAGAAAAGTTAATGACTATCAAAATTAT
  TCATGACTTTGAGCTCAACAAGTTGAAAGAAAACTTGGATGTTGAATATGGTAACATCATGAAGAATGTT
  TTAGTAGATGAAGACAATTCCCTGAAGGCTAAAACTACAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231263 representing NM_001271879
  Red=Cloning site Green=Tags(s)
  
  MVEQEGLTAKEIDYAFQQNENLGSKETSFLIPEDLQSPFQRSEHSKDSVFFRDGIRQIDFVLSYVEDLKK
  DGELKAERRREFEQNLRKTGLDLETEDKLNSEDGKTYFVKIHAPWEVLVTYAEVLGIKMPIKLSDIPRPK
  YPPLSYMLGAVKLPSSVKYPTPEYFTAQFSRHRQELFLIEDEATFFPSSTRNRIVYYILSRCPFGVEEGK
  KKIGIERLLNSNTYLSAYPLHDGQYWKPSKTTRPNERYNLCKNWARFSYFYKEQPFHLIRNYFGEKIGIY
  FVFLGYYTEMLLFAALVGLACFIYGLLSMENNRTSTEICDPDIGGQMIMCPLCDEVCDYWRLNTTCLHSK
  FSHLFDNESTVFFALFMGIWVTLFLEFWKQRQARLEYEWDLVDFEEEQQQLQLRPEFEAMCKHKKMNPVT
  KEMEPHMPLCHRIPWYFVSGTTVTFGMALLLSSMVSILIYRLSVFATFASFMESEATLQSVKSFFTPQLA
  TALSGSCLNCIVILILNFFYEKISAWITKMGKFVGYPGSYTYMFNIWRSEECGPAGCLIELTTQLTIIMI
  GKQIFGNIHEAFQPLIFNWWRRRRARTHSEKLYSRWEQDHDLQVYGHRGLFYEYLETVIQFGFATLFVAS
  FPLAPLFALMNNIMGIRVDAWKLTTQYRRPVAAKAHSIGVWQDILFGMAIVSVATNAFIVSFTSDIIPRL
  VYFYAYSTNSTEPLSGYVNNSLSVFLIADFPNHTVPMEKKDFVTCRYRDYRYPPDHEDKYSHNMQFWHVL
  AAKMTFIIVMEHVVFLFKFLLAWLIPDVPKDVVEKIKREKLMTIKIIHDFELNKLKENLDVEYGNIMKNV
  LVDEDNSLKAKTTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271879
• ORF Size: 2562 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001271879.1, NP_001258808.1
• RefSeq Size: 7633
• RefSeq ORF: 2565
• Locus ID: 233246
• MW: 100.4 kDa
• Gene Summary: This gene encodes a member of the anoctamin family, which in mammals is comprised of 10 members. Anoctamin proteins are proposed to have eight transmembrane domains with both termini facing the cytoplasm and a C-terminal domain of unknown function. While some members have been characterized as calcium-activated chloride channels, this protein is reported to have little anion conductance activity. Elevated levels of this protein were found in dystrophic mice. In humans, mutations of this gene are associated with with musculoskeletal disorders such as myopathies, muscular dystrophy and gnathodiaphyseal dysplasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]