Grip1 (NM_001277294) Mouse Tagged ORF Clone

CAT#: MR231536

Grip1 (myc-DDK-tagged) - Mouse glutamate receptor interacting protein 1 (Grip1), transcript variant 6

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Grip1
• Synonyms: 4931400F03Rik; eb; GRIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231536 representing NM_001277294
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAGCTGTCTCTTTTAAATGCCGCTGTCAAATTCTAAGGCGACTTACCAAAGATGAGAGTCCCTACA
  CTAAATCTGCCAGCCAGACAAAGCCGCCCGATGGAGCATTGGCTGTGAGGAGACAGAGCATCCCAGAGGA
  ATTCAAGGGCTCCACAGTGGTGGAGTTGATGAAGAAGGAGGGAACCACTCTTGGCCTGACGGTATCGGGA
  GGCATTGATAAAGATGGCAAGCCAAGAGTGTCCAACCTGCGGCAGGGAGGAATCGCTGCCAGAAGTGACC
  AGCTGGATGTGGGCGACTACATCAAGGCGGTGAATGGGATCAACCTGGCCAAGTTCCGCCACGATGAGAT
  CATCAGCCTGCTGAAAAATGTCGGGGAAAGAGTGGTCCTGGAGGTCGAGTACGAGCTTCCACCGGTCTCT
  GTACAAGGATCCAGTGTTATGTTCCGAACTGTGGAGGTCACGCTGCACAAAGAAGGCAACACCTTTGGTT
  TTGTCATCCGAGGGGGAGCGCATGATGACAGGAACAAGTCCCGTCCGGTTGTGATAACCTGTGTTCGTCC
  TGGAGGGCCTGCTGACAGAGAGGGCACCATCAAACCTGGAGACAGGTTGCTCAGCGTGGATGGAATTCGG
  CTCCTGGGAACCACCCATGCCGAGGCCATGAGCATCCTTAAACAGTGCGGACAAGAAGCCACGCTGCTGA
  TAGAATACGATGTCTCTGTGATGGATTCTGTGGCGACAGCATCCGGGCCACTACTAGTTGAAGTTGCCAA
  AACTCCAGGTGCCAGCCTTGGGGTTGCCCTAACTACCTCCGTGTGCTGTAACAAGCAGGTCATTGTCATA
  GACAAAATCAAATCTGCAAGCATTGCGGACAGATGTGGCGCGCTACACGTGGGAGACCACATCCTCTCCA
  TTGACGGCACGAGTATGGAGTACTGTACCCTCGCAGAAGCGACCCAGTTCCTGGCCAATACCACTGACCA
  GGTCAAGCTGGAGATTCTCCCACACCATCAGACCCGCCTGGCCCTAAAGGGCCCTGACCATGCGGCTATG
  GTGCCCTCATCTTCTCCTACCTCCATGAGTGCGTACAGTCTGAGTTCCCTGAACATGGGGACTTTACCTC
  GAAGCCTCTACTCCACCAGCCCACGAGGAACCATGATGAGGAGGAGACTGAAAAAGAAAGACTTCAAAAG
  CTCACTGTCTTTAGCCTCCAGCACTGTGGGGTTGGCTGGCCAGGTCGTTCACACTGAAACCACAGAGGTT
  GTGCTGACGGCTGACCCTGTCACGGGCTTCGGAATCCAACTGCAGGGCAGCGTGTTTGCCACAGAGACGC
  TCTCCTCTCCGCCTCTGATTTCCTATATTGAAGCTGACAGCCCAGCAGAGAGATGTGGTGTGCTACAGAT
  TGGAGACAGAGTCATGGCCATTAATGGAATCCCAACAGAAGACAGCACCTTCGAGGAAGCCAATCAACTC
  CTGAGAGACTCTTCCATCACGAGCAAAGTCACACTAGAAATCGAGTTTGATGTTGCAGAGTCTGTCATCC
  CAAGTAGTGGAACATTTCATGTAAAACTGCCCAAGAAGCACAGCGTGGAACTTGGAATAACCATCAGTTC
  GCCATCCAGTAGAAAACCGGGGGACCCCCTTGTCATTTCAGATATCAAGAAAGGCAGTGTGGCACACAGA
  ACCGGAACTCTGGAACTGGGAGACAAACTGCTTGCGATAGATAACATCCGGCTGGATAACTGTTCCATGG
  AAGATGCGGTCCAGATCCTCCAGCAGTGTGAAGACCTGGTGAAGCTCAAAATCCGCAAAGATGAAGATAA
  CTCAGACGAGCAAGAGAGTTCCGGAGCGATTATTTACACGGTGGAGCTGAAGCGCTATGGGGGGCCCCTT
  GGCATCACAATTTCTGGAACTGAAGAGCCGTTTGATCCTATTATCATCTCGAGCCTCACTAAAGGGGGAT
  TAGCTGAAAGGACTGGAGCGATCCACATCGGAGATAGAATCCTAGCCATCAATAGCAGCAGCTTGAAGGG
  GAAGCCTCTGAGTGAAGCCATCCACTTGCTCCAGATGGCAGGAGAGACTGTCACCCTGAAAATTAAGAAA
  CAGACAGATGCCCAATCTGCATCAAGTCCCAAGAAGTTCCCCATCCCTGGCCACTCGGGGGACCTAGGAG
  ATGGTGAGGAGGACCCCTCCCCAATACAGAAACCTGGCAAGCTCTCCGATGCGTACCCCTCCACGGTGCC
  CAGCGTGGACAGTGCTGTGGACTCCTGGGATGGGTCTGGAATAGATGCCAGCTATGGGAGTCAAGGCTCA
  ACTTTTCAGACTTCAGGATACAATTACAACACCTATGATTGGAGGAGTCCAAAGCAAAGAACCAGCCTGT
  CCCCAGTCCCCAAGCCTCGAAGCCAGACGTACCCAGATGTGGGCCTGAGTAATGAAGACTGGGATCGATC
  CACAGCCAGTGGCTTTGTAGGGGCTTCTGACAGTGCAGATGCTGAACAAGAGGAAAACTTCTGGTCTCAA
  GCATTGGAGGACCTGGAGACCTGCGGCCAGTCGGGGATCCTGAGAGAGCTTGAGGCAACAATCATGTCGG
  GGAGTACTATGAGTTTGAATCATGAGGCTCCAATGGCTCGCAGTCAGCTGGGGCGACAGGCCAGCTTCCA
  GGAACGGAGCAGTTCACGGCCACACTATAGCCAAACAACTCGCAGCAACACCCTGCCCTCAGACGTGGGC
  AGAAAGTCTGTAACCCTGCGGAAAATGAAGCAAGAAATAAAGGAGATCATGTCCCCAACTCCGGTGGAGC
  TACACAAGGTGACCTTATACAAGGACTCTGGCATGGAGGACTTCGGGTTCAGTGTGGCAGATGGCCTGCT
  GGAGAAAGGCGTGTATGTCAAAAATATCCGCCCAGCTGGGCCAGGTGATGTTGGGGGCTTGAAGCCCTAC
  GACAGGCTCTTACAGGTAAATCACGTGCGGACGAGAGACTTTGACTGCTGCCTGGTGGTGCCTCTCATAG
  CTGAATCTGGCAACAAGCTGGACCTGGTTATTAGCAGAAATCCACTGGCCTCCCAGAAGTCGATAGAACA
  GCCGGCTCTGCCCAGCGACTGGAGCGAACAGAACAGCGCTTTCTTCCAGCAACCCAGCCACGGTGGTAAT
  CTAGAGACACGAGAACCCACTAACACACTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231536 representing NM_001277294
  Red=Cloning site Green=Tags(s)
  
  MIAVSFKCRCQILRRLTKDESPYTKSASQTKPPDGALAVRRQSIPEEFKGSTVVELMKKEGTTLGLTVSG
  GIDKDGKPRVSNLRQGGIAARSDQLDVGDYIKAVNGINLAKFRHDEIISLLKNVGERVVLEVEYELPPVS
  VQGSSVMFRTVEVTLHKEGNTFGFVIRGGAHDDRNKSRPVVITCVRPGGPADREGTIKPGDRLLSVDGIR
  LLGTTHAEAMSILKQCGQEATLLIEYDVSVMDSVATASGPLLVEVAKTPGASLGVALTTSVCCNKQVIVI
  DKIKSASIADRCGALHVGDHILSIDGTSMEYCTLAEATQFLANTTDQVKLEILPHHQTRLALKGPDHAAM
  VPSSSPTSMSAYSLSSLNMGTLPRSLYSTSPRGTMMRRRLKKKDFKSSLSLASSTVGLAGQVVHTETTEV
  VLTADPVTGFGIQLQGSVFATETLSSPPLISYIEADSPAERCGVLQIGDRVMAINGIPTEDSTFEEANQL
  LRDSSITSKVTLEIEFDVAESVIPSSGTFHVKLPKKHSVELGITISSPSSRKPGDPLVISDIKKGSVAHR
  TGTLELGDKLLAIDNIRLDNCSMEDAVQILQQCEDLVKLKIRKDEDNSDEQESSGAIIYTVELKRYGGPL
  GITISGTEEPFDPIIISSLTKGGLAERTGAIHIGDRILAINSSSLKGKPLSEAIHLLQMAGETVTLKIKK
  QTDAQSASSPKKFPIPGHSGDLGDGEEDPSPIQKPGKLSDAYPSTVPSVDSAVDSWDGSGIDASYGSQGS
  TFQTSGYNYNTYDWRSPKQRTSLSPVPKPRSQTYPDVGLSNEDWDRSTASGFVGASDSADAEQEENFWSQ
  ALEDLETCGQSGILRELEATIMSGSTMSLNHEAPMARSQLGRQASFQERSSSRPHYSQTTRSNTLPSDVG
  RKSVTLRKMKQEIKEIMSPTPVELHKVTLYKDSGMEDFGFSVADGLLEKGVYVKNIRPAGPGDVGGLKPY
  DRLLQVNHVRTRDFDCCLVVPLIAESGNKLDLVISRNPLASQKSIEQPALPSDWSEQNSAFFQQPSHGGN
  LETREPTNTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001277294
• ORF Size: 3180 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001277294.1, NP_001264223.1
• RefSeq Size: 5018
• RefSeq ORF: 3183
• Locus ID: 74053
• MW: 114.7 kDa
• Gene Summary: This gene encodes a protein containing multiple PDZ (post synaptic density protein, Drosophila disc large tumor suppressor, and zonula occludens-1 protein) domains. The encoded protein acts as a mediator between cytoskeletal and membrane proteins, particularly in neuronal cells, and facilitates complex formation at the cell membrane. Mutation of this gene can cause embryonic lethality resulting from defects of the dermo-epidermal junction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]