Adcy7 (NM_001037723) Mouse Tagged ORF Clone

CAT#: MR231579

Adcy7 (myc-DDK-tagged) - Mouse adenylate cyclase 7 (Adcy7), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adcy7
• Synonyms: AA407758
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231579 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCCAAGGGGCGCTACTTCCTAAATGAGGGTGATGAAGGCCCCGACCAGGCAGCGCTCTATGAGA
  AGTACCGGCTCACCAGCTTGCACGGGCCACTGCTGCTCTTGCTCCTCCTGGTGGCCGCGGCCACCTGCAT
  TGCGCTCATCAGCATCGCCTTCAGTCATGAGGATCTCCGCAGACACCAGGTTGTCCTGGGCACTGCGTTC
  CTCATGCTGACGCTGTTTGTGGCTCTCTATGTGCTGGTGTATGTCGAGTGCCTGGTGCAGCGGTGGCTGC
  GGGCCTTGGCGCTACTCACCTGGGCTTGCCTCATGGTACTAGGCTCCGTGCTGATGTGGGACTCTTTGGA
  GAATGAAGCCCATGCGTGGGAGCAGGTGCCTTTCTTCCTGTTTGTCGTCTTTGTGGTGTATGCACTACTG
  CCTCTCAGCAGGAGGGCAGCCATCGTGGCAGGCGTGACCTCCACGGTCTCCCATCTCCTGGTGTTTGGAG
  CTGTGACAAGAGCCTTCCAGACGTCCATGTCTAGCACTCAACTGGGGCTGCAGCTCCTGGCCAATGCCGT
  TATCCTCCTGGGTGGGAACTTCACGGGTGCCTTCCACAAGCACCAGCTGCAGGACGCGTCCAGGGATCTC
  TTTATCTACACCGTCAAATGCATCCAGATCCGTCGGAAGCTTCGTGTGGAGAAGCGCCAGCAGGAGAACC
  TGCTTCTGTCAGTGCTCCCAGCACACATCTCCATGGGTATGAAGCTGGCCATCATTGAGCGCCTCAAAGA
  GGGTGGTGACCGACACTACATGCCCGACAACAACTTTCACAGCCTCTATGTCAAGCGGCACCAGAATGTC
  AGCATCTTGTATGCAGACATCGTGGGCTTCACGAGGCTGGCCAGCGACTGCTCTCCCAAGGAGCTGGTGG
  TGGTGCTCAACGAGCTGTTTGGGAAGTTTGACCAGATTGCTAAGGCCAATGAGTGCATGCGGATCAAGAT
  CCTGGGTGACTGTTACTACTGCGTGTCAGGCCTGCCCGTGTCGCTGCCCACACATGCCCGCAACTGTGTG
  AAGATGGGTCTGGACATCTGCGAGGCCATTAAGCAGGTGCGTGAGGCCACGGGCGTGGACATCAGCATGC
  GTGTGGGCATTCACTCCGGGAATGTGCTATGTGGGGTCATCGGGCTCCGTAAGTGGCAGTATGATGTGTG
  GTCCCATGATGTGTCCCTGGCCAACAGGATGGAGGCAGCTGGAGTCCCTGGCCGGGTGCACATCACAGAG
  GCAACATTGAATCACCTGGACAAGGCATATGAGGTGGAGGATGGGCATGGGGAGCAGCGAGACCCCTATC
  TGAAAGAGATGAACATCCGAACCTACCTGGTGATCGATCCCCGGAGCCAGCAGCCACCCCCACCCAGCCA
  CCACCTCTCCAAGCCCAAGGGGGACGCAACTCTGAAGATGCGGGCTTCAGTGCGTGTAACCCGCTATCTG
  GAGTCTTGGGGGGCAGCAAGGCCCTTTGCACACCTCAACCACCGGGAGAGTGTGAGCAGCAGTGAGACCC
  CCATCTCCAATGGACGGAGGCAGAAGGCCATTCCTCTGCGTCGACACCGTGCCCCTGATAGGAGTGCATC
  TCCCAAGGGGCGCTTGGAAGATGACTGTGATGACGAGATGCTGTCAGCCATTGAGGGTCTCAGCTCCACC
  AGGCCCTGCTGCTCCAAGTCTGATGACTTCCACACCTTTGGTCCCATCTTCTTGGAGAAGGGCTTTGAGC
  GTGAGTACCGCCTGGTGCCCATCCCCCGGGCTCGCTACGACTTCGCCTGTGCCAGCCTTGTCTTCGTCTG
  CATCCTGCTTGTCCACCTTCTAGTGATGCCCAGGATGGCAACTCTGGGTGTGTCCTTTGGGTTGGTGGCC
  TGCCTGCTGGGGCTGGTTCTGAGTTTCTGCTTTGCTACTGAGTTCTCGAGGTGCTTTCCATCCCGAAGTA
  CACTCCAGGCCATCTCGGAGAGCGTGGAGACGCAGCCCCTGGTCAGGCTTGTCCTGGTTGTGCTGACTGT
  TGGCAGCCTACTGACTGTCGCCATCATTAACATGCCACTGACGCTTAACCCAGGCCCAGAGCAGCCTGGA
  GACAACAAGACAAGCCCACTGGCTGCACAGAACAGAGTTGGGACCCCATGTGAGCTCCTCCCGTACTACA
  CCTGCAGCTGCATCCTGGGCTTCATTGCATGCTCTGTTTTCCTGCGGATGAGCCTAGAGCTGAAGGCCAT
  GCTGCTGACAGTGGCCTTGGTGGCCTACCTGCTGCTCTTCAACCTCTCCCCATGCTGGCACGTCTCAGGC
  AACAGCACTGAGACCAACGGGACACAAAGGACACGGCTGCTCCTGTCTGATGCACAAAGCATGCCCAGCC
  ACACCCTTGCTCCGGGGGCTCGGGAGACTGCCCCTTCTCCCAGTTATTTAGAGAGAGACCTGAAGATCAT
  GGTTAACTTCTACCTGATCCTGTTCTATGCCACCCTCATCTTGCTGTCTAGACAGATTGACTACTACTGC
  CGCTTGGACTGTCTGTGGAAGAAGAAGTTCAAAAAGGAGCACGAGGAGTTTGAAACAATGGAGAATGTGA
  ACCGCCTCCTCCTGGAGAATGTGCTGCCGGCGCACGTGGCTGCCCACTTCATTGGGGACAAGGCAGCAGA
  GGATTGGTACCATCAATCTTATGACTGTGTCTGTGTCATGTTTGCATCCGTTCCGGACTTCAAAGTGTTC
  TACACTGAGTGTGATGTCAACAAAGAAGGACTGGAGTGCCTTCGACTGCTGAATGAGATAATTGCTGATT
  TTGACGAGCTCCTGCTGAAGCCCAAGTTTAGTGGTGTGGAGAAGATCAAGACCATTGGCAGCACCTACAT
  GGCGGCAGCAGGGCTCAGTGCCCCCTCAGGACATGAGAACCAGGACCTGGAGCGGAAGCACGTGCACATC
  GGAGTCTTGGTAGAATTTAGCATGGCCCTGATGAGCAAGCTGGATGGGATCAACAGGCACTCCTTCAACT
  CCTTCCGCCTCCGAGTCGGCATAAACCACGGGCCTGTGATTGCTGGAGTGATTGGAGCACGCAAGCCTCA
  GTATGACATCTGGGGAAACACAGTCAATGTTGCCAGCCGCATGGAGAGCACCGGAGAGCTTGGGAAAATC
  CAGGTTACCGAAGAGACATGCACTATCCTCCAGGGACTCGGATATTCGTGTGAATGCCGTGGGCTGATCA
  ACGTCAAAGGCAAAGGGGAACTGCGGACTTACTTTGTATGTACAGACACTGCCAAGTTTCAAGGGCTGGG
  GCTAAAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231579 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPAKGRYFLNEGDEGPDQAALYEKYRLTSLHGPLLLLLLLVAAATCIALISIAFSHEDLRRHQVVLGTAF
  LMLTLFVALYVLVYVECLVQRWLRALALLTWACLMVLGSVLMWDSLENEAHAWEQVPFFLFVVFVVYALL
  PLSRRAAIVAGVTSTVSHLLVFGAVTRAFQTSMSSTQLGLQLLANAVILLGGNFTGAFHKHQLQDASRDL
  FIYTVKCIQIRRKLRVEKRQQENLLLSVLPAHISMGMKLAIIERLKEGGDRHYMPDNNFHSLYVKRHQNV
  SILYADIVGFTRLASDCSPKELVVVLNELFGKFDQIAKANECMRIKILGDCYYCVSGLPVSLPTHARNCV
  KMGLDICEAIKQVREATGVDISMRVGIHSGNVLCGVIGLRKWQYDVWSHDVSLANRMEAAGVPGRVHITE
  ATLNHLDKAYEVEDGHGEQRDPYLKEMNIRTYLVIDPRSQQPPPPSHHLSKPKGDATLKMRASVRVTRYL
  ESWGAARPFAHLNHRESVSSSETPISNGRRQKAIPLRRHRAPDRSASPKGRLEDDCDDEMLSAIEGLSST
  RPCCSKSDDFHTFGPIFLEKGFEREYRLVPIPRARYDFACASLVFVCILLVHLLVMPRMATLGVSFGLVA
  CLLGLVLSFCFATEFSRCFPSRSTLQAISESVETQPLVRLVLVVLTVGSLLTVAIINMPLTLNPGPEQPG
  DNKTSPLAAQNRVGTPCELLPYYTCSCILGFIACSVFLRMSLELKAMLLTVALVAYLLLFNLSPCWHVSG
  NSTETNGTQRTRLLLSDAQSMPSHTLAPGARETAPSPSYLERDLKIMVNFYLILFYATLILLSRQIDYYC
  RLDCLWKKKFKKEHEEFETMENVNRLLLENVLPAHVAAHFIGDKAAEDWYHQSYDCVCVMFASVPDFKVF
  YTECDVNKEGLECLRLLNEIIADFDELLLKPKFSGVEKIKTIGSTYMAAAGLSAPSGHENQDLERKHVHI
  GVLVEFSMALMSKLDGINRHSFNSFRLRVGINHGPVIAGVIGARKPQYDIWGNTVNVASRMESTGELGKI
  QVTEETCTILQGLGYSCECRGLINVKGKGELRTYFVCTDTAKFQGLGLN
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037723
• ORF Size: 3297 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037723.1, NM_001037723.2, NM_001037723.3, NP_001032812.2
• RefSeq Size: 6033 bp
• RefSeq ORF: 3300 bp
• Locus ID: 11513
• Cytogenetics: 8 43.06 cM
• MW: 122.7 kDa
• Gene Summary: This is a membrane-bound, calcium-inhibitable adenylyl cyclase. [UniProtKB/Swiss-Prot Function]