Asap1 (NM_001276462) Mouse Tagged ORF Clone

CAT#: MR231602

Asap1 (myc-DDK-tagged) - Mouse ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asap1
• Synonyms: AV239055; Ddef1; DEF-1; mKIAA1249; PAP; s19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231602 representing NM_001276462
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGACCAGATCTCCGTGTCCGAGTTCATCGCCGAGACCACCGAGGACTACAACTCGCCCACCACGT
  CCAGCTTCACCACGCGGCTGCACAACTGCAGGAACACCGTCACGCTGCTGGAGGAGGCTCTAGACCAAGA
  TAGAACAGCCCTACAGAAAGTGAAGAAATCTGTAAAAGCAATATATAATTCCGGTCAAGATCATGTACAA
  AATGAAGAAAACTATGCACAAGTTCTGGATAAGTTTGGAAGTAATTTTTTAAGCAGAGACAACCCTGACC
  TTGGCACCGCTTTTGTCAAGTTTTCTACACTCACAAAGGAACTGTCCACGCTGCTGAAAAATCTGCTCCA
  GGGTCTGAGCCACAATGTGATCTTCACCTTGGATTCCTTGTTAAAAGGAGACTTGAAGGGGGTCAAAGGA
  GATCTCAAGAAGCCATTTGACAAAGCCTGGAAAGATTATGAGACGAAGTTTACAAAAATTGAGAAGGAGA
  AAAGAGAGCACGCAAAACAGCACGGGATGATCCGAACAGAGATAACAGGCGCTGAGATTGCAGAAGAAAT
  GGAAAAGGAACGGCGGCTTTTTCAGCTCCAGATGTGTGAATATCTCATTAAAGTAAATGAAATCAAGACC
  AAAAAGGGTGTGGACCTGCTGCAGAACCTTATAAAGTATTACCATGCACAGTGCAATTTCTTTCAGGATG
  GCTTGAAAACGGCTGATAAACTGAAACAGTACATAGAAAAGCTGGCTGCTGACTTATATAATATAAAGCA
  AACCCAAGATGAAGAAAAAAAACAGCTAACTGCACTCCGAGATCTAATAAAATCCTCTCTGCAACTTGAT
  CCGAAAGAAGTAGGTGGTTTATATGTTGCCAGCAGGGCTAACAGTGACTCTCAGAGCCGGCAAGGTGGGT
  ACAGCATGCATCAGCTCCAGGGCAACAAGGAGTACGGCAGTGAAAAGAAGGGCTTCCTGCTGAAGAAGAG
  TGATGGGATCCGGAAAGTGTGGCAGAGACGGAAGTGTGCGGTCAAGAACGGGATCCTGACCATCTCCCAC
  GCAACTTCCAACAGGCAGCCTGCTAAGTTAAACCTCCTCACCTGCCAAGTGAAACCGAATGCTGAGGACA
  AGAAGTCTTTTGACCTGATATCACATAATCGAACATATCACTTTCAAGCAGAAGATGAGCAGGATTATAT
  AGCGTGGATATCAGTACTGACGAATAGCAAAGAAGAGGCCCTAACCATGGCCTTCCGTGGTGAGCAGAGC
  ACAGGGGAGAATAGCCTGGAGGACCTTACCAAAGCCATCATCGAGGATGTACAGCGGCTCCCTGGGAATG
  ACATCTGCTGTGACTGCGGCTCATCAGAACCCACGTGGCTTTCAACCAACTTGGGTATTTTGACCTGTAT
  AGAATGTTCCGGAATCCATAGGGAAATGGGGGTTCATATTTCTCGCATTCAGTCTTTGGAACTAGACAAA
  TTAGGAACTTCCGAACTCTTGCTGGCCAAGAATGTAGGAAACAATAGTTTTAATGATATTATGGAAGCAA
  ATTTACCCAGCCCTTCACCAAAGCCCACCCCTTCAAGTGATATGACCGTGCGTAAAGAGTACATCACTGC
  AAAGTATGTCGACCACCGCTTCTCCAGGAAGACCTGTGCCTCTTCCTCAGCAAAGCTGAACGAATTGCTT
  GAGGCCATCAAGTCCAGGGACTTACTTGCATTAATCCAAGTCTATGCAGAGGGGGTGGAGTTAATGGAGC
  CACTGCTGGAACCTGGGCAGGAGCTCGGGGAGACAGCCCTTCATCTTGCCGTCAGGACCGCAGACCAGAC
  ATCTCTCCATCTGGTTGATTTCCTTGTACAAAACTGTGGGAACCTGGATAAGCAAACGTCTGTGGGGAAT
  ACAGTTCTGCACTATTGCAGCATGTACGGCAAGCCGGAGTGTCTGAAGCTGCTGCTTAGGAGCAAGCCCA
  CCGTGGACATCGTTAACCAGAATGGAGAAACTGCCTTGGATATAGCAAAGAGACTCAAAGCTACCCAGTG
  TGAAGATCTGCTTTCCCAGGCTAAATCTGGAAAGTTCAACCCTCATGTCCACGTAGAGTATGAGTGGAAT
  CTTCGACAGGACGAGATGGATGAGAGCGATGACGATCTGGATGACAAGCCAAGCCCGATCAAAAAGGAAC
  GCTCGCCCAGACCACAGAGCTTCTGCCACTCCTCCAGCATCTCGCCACAGGACAAGCTGGCACTGCCAGG
  GTTCAGCACTCCACGGGACAAGCAGCGGCTCTCCTACGGAGCCTTCACCAACCAGATCTTCGCCTCTACG
  AGCACAGATTTGCCCACATCACCCACCAGTGAGGCTCCCCCTTTGCCACCTCGGAACGCCGGGAAAGGTC
  CAACTGGCCCACCTTCAACACTCCCTCTAGGCACCCAGACCTCTAGTGGCAGCTCCACCCTATCCAAGAA
  GAGGCCTCCTCCCCCACCACCAGGACACAAGAGAACCCTGTCTGACCCTCCCAGCCCACTACCTCACGGG
  CCCCCAAACAAAGGCGCAATTCCTTGGGGTAATGATGTGGGCCCATTATCTTCAAGTAAGACGGCCAACA
  AGTTTGAGGGGCTGTCTCAGCAAGCAAGCACCAGTTCTGCTAAGACTGCCCTTGGCCCGAGAGTGCTTCC
  TAAACTACCTCAGAAAGTGGCACTAAGGAAGACGGAGACCAGCCATCATCTCTCCCTCGACAGAACCAAC
  ATCCCACCTGAGACTTTTCAGAAATCATCACAGTTGACAGAGTTACCCCAAAAGCCACCACTTGGAGAGC
  TGCCCCCGAAGCCTGTGGAACTGGCCCCCAAGCCCCAAGTTGGAGAGCTGCCACCTAAGCCTGGAGAGCT
  ACCCCCTAAGCCCCAATTAGGTGACCTGCCCCCCAAGCCACAGCTCTCAGACTTACCTCCCAAGCCACAG
  ATGAAGGACCTGCCCCCTAAGCCGCAGCTGGGGGATCTGCTGGCAAAGTCCCAGGCTGGCGATGTCTCAG
  CCAAGGTGCAGCCACCCTCAGAGGTCACACAGAGGTCACACACCGGGGATCTGTCTCCAAATGTACAGTC
  CAGAGATGCCATCCAGAAGCAAGCATCTGAAGACTCCAACGACCTCACACCCACGCTGCCAGAGACACCC
  GTACCACTGCCGAGAAAAATCAATACGGGGAAAAATAAAGTGAGGCGGGTGAAGACCATTTATGACTGCC
  AGGCAGATAATGACGACGAACTCACATTTATTGAGGGGGAAGTGATCATTGTCACCGGGGAAGAGGACCA
  GGAGTGGTGGATCGGGCATATCGAAGGACAGCCTGAAAGGAAGGGTGTCTTTCCAGTGTCCTTTGTCCAC
  ATCCTGTCTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231602 representing NM_001276462
  Red=Cloning site Green=Tags(s)
  
  MPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQ
  NEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKG
  DLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKT
  KKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLD
  PKEVGGLYVASRANSDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISH
  ATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQS
  TGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDK
  LGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELL
  EAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGN
  TVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWN
  LRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFAST
  STDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHG
  PPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTN
  IPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQ
  MKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETP
  VPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVH
  ILSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276462
• ORF Size: 3372 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001276462.1, NP_001263391.1
• RefSeq Size: 6181
• RefSeq ORF: 3375
• Locus ID: 13196
• MW: 125.2 kDa