Nek1 (NM_001293638) Mouse Tagged ORF Clone

CAT#: MR231703

Nek1 (myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nek1
• Synonyms: D8Ertd790e; kat
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231703 representing NM_001293638
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGTATGTGAGACTGCAGAAGATTGGAGAAGGTTCATTTGGAAAAGCTGTTCTTGTTAAATCGA
  CAGAGGATGGCAGACATTATGTCATCAAGGAAATTAACATCTCAAGAATGTCTGATAAAGAAAGGCAAGA
  ATCAAGGAGAGAAGTTGCTGTATTGGCAAACATGAAGCATCCAAATATTGTCCAATATAAAGAATCATTT
  GAAGAAAATGGCTCTCTCTACATAGTAATGGATTACTGTGAAGGAGGTGATTTGTTTAAACGAATAAATG
  CTCAGAAAGGCGCTCTGTTTCAAGAAGACCAGATTTTGGACTGGTTTGTGCAGATATGTTTGGCTCTGAA
  GCATGTACATGATAGAAAAATTCTTCACCGAGACATAAAGTCACAGAACATATTTCTAACCAAAGATGGG
  ACAGTGCAGCTTGGAGATTTTGGAATTGCTCGAGTTCTTAATAGTACTGTAGAGCTGGCTCGAACTTGCA
  TAGGCACTCCATACTACTTGTCACCTGAAATCTGTGAAAACAAGCCTTATAACAATAAAAGTGACATTTG
  GGCTTTGGGCTGTGTCCTTTATGAGTTGTGTACACTTAAACATGCATTTGAAGCTGGAAACATGAAAAAC
  CTGGTACTGAAGATAATCTCCGGATCCTTTCCTCCAGTGTCTCCACATTACTCCTATGATCTCCGCAGCT
  TGCTGTCTCAGTTATTTAAAAGAAATCCTAGGGATAGACCATCAGTCAACTCCATATTGGAGAAAGGTTT
  TATAGCTAAACGAATCGAAAAGTTTCTCTCCCCTCAGCTTATTGCAGAAGAATTTTGTCTAAAAACACTT
  TCAAAGTTTGGACCACAGCCTCTCCCAGGTAAAAGACCAGCATCAGGACAAGGTGTCAGTTCTTTTGTCC
  CTGCTCAGAAAATCACAAAGCCTGCTGCTAAATACGGAGTGCCTTTAACATATAAGAAGTATGGAGATAA
  AAAGTTACTTGAGAAAAAACCACCCCCAAAACATAAACAGGCCCATCAAATTCCCGTGAAGAAAATGAAT
  TCTGGAGAAGAAAGGAAGAAAATGTCTGAGGAAGCAGCAAAAAAAAGAAGGTTGGAATTTATTGAGAAAG
  AAAAGAAGCAAAAGGATCAGATTAGGTTCCTGAAGGCTGAGCAGATGAAGCGGCAAGAGAAGCAGCGGTT
  GGAGAGGATAAATAGGGCCAGGGAACAAGGATGGAGGAATGTTTTAAGGGCTGGTGGAAGCGGTGAAGTA
  AAGGCTTCCTTTTTTGGCATTGGAGGGGCTGTCTCTCCATCACCGTGTTCTCCTCGAGGCCAGTATGAAC
  ATTACCATGCCATTTTTGACCAAATGCAGCGGCTAAGAGCAGAAGATAATGAAGCAAGATGGAAGGGGGG
  AATCTATGGTCGATGGCTCCCAGAAAGGCAAAAAGGACACTTAGCTGTAGAGAGAGCCAACCAAGTGGAA
  GAATTCCTACAGCGTAAACGAGAAGCTATGCAGAATAAAGCCCGAGCCGAAGGACACGTGGGACTCCTGC
  AAAACCTGGCATCTCTGTATGGAGGCAGGCCCAGCTCTTCAAGAGGAGGGAAGCCAAGGAACAATGAGGA
  AGAGGTTTATTTGGCAAGACTGAGGCAAATAAGACTACAAAATTTTAATGAGCGCCAACAGATTAAAGCC
  AAACTTCGTGGTGAGAATAAAGAAGCTGATGGTACCAAAGGACAAGAAGCAACTGAAGAGACTGACATGA
  GGCTCAAAAAGATGGAGTCACTTAAGGCGCAAACAAATGCACGTGCTGCTGTACTAAAAGAACAGCTGGA
  GCGAAAAAGAAAGGAAGCTTATGAAAGAGAAAAGAAAGTATGGGAAGAACATTTGGTGGCGAGGGTAAAA
  AGCTCAGATGTTCCTCTGCCTTTGGAACTTCTTGAAACAGGTGGTTCTCCATCAAAGCAGCAGGTGAAGC
  CTGTCATTTCTGTGACTTCAGCTTTGAAAGAAGTGGGCCTGGATGGAAGTTTAACTGATACCCAGGAAGA
  AGAAATGGAAAAGAGTAACAGTGCTATTTCAAGTAAGCGAGAAATCCTGCGTAGGCTAAATGAAAATCTT
  AAAGCTCAAGAGGATGAAAAGGAAAAGCAGCATCACTCAGGTTCTTGTGAGACCGTTGGTCACAAAGATG
  AGAGAGAGTATGAGACAGAAAATGCCATTTCCTCTGATCGCAAGAAGTGGGAGATGGGAGGTCAGCTTGT
  GATTCCTCTCGATGCAGTGACACTGGATACATCCTTCTCTGCAACCGAAAAACATACTGTGGGAGAGGTT
  ATTAAATTAGATTCTAATGGCTCTCCAAGAAAAGTCTGGGGGAAAAACCCTACAGATTCTGTGCTGAAGA
  TACTTGGAGAAGCTGAATTACAGCTACAGACAGAACTACTAGAAAACACATCTTTTAAAAGTGAGGTTTA
  TGCTGAAGAGGAGAACTACAAACCCTTACTTACTGAAGAAGAGAATCTGCAGTGCATTTCAAAAGAAATA
  AATCCATCAGCTACTGTTGATTCTACTGAAACGAAAAGTCCAAAGTTTACTGAGGTGTCTCCACAAATGT
  CAGAAGGAAATGTGGAAGAACCTGATGATTTGGAAACAGAAGTTCTACAAGAGCCAAGTAGCACACACAC
  AGATGGGAGTTTGCCACCTGTTCTTAATGATGTGTGGACTAGAGAGAAGGAAGCAGCTAAGGAAACTGAG
  TTGGAAGATAAGGTTGCTGTGCAGCAGAGTGAAGTTTGTGAAGATAGAATTCCAGGGAACGTGGACCAAT
  CCTGTAAGGATCAGAGAGATCCTGCAGTAGACGATTCTCCGCAGTCTGGCTGTGATGTAGAGAAGTCAGT
  ACAGCCAGAATCGATTTTCCAGAAAGTGGTTCATTCTAAGGACTTGAACTTAGTTCAGGCAGTTCATTGC
  TCACCAGAAGAACCAATTCCAATTCGATCTCACTCTGATTCTCCACCAAAAACTAAGAGCAAGAATTCCT
  TACTGATTGGACTTTCAACTGGTCTGTTTGATGCAAACAATCCAAAGATGCTGAGGACCTGCTCACTTCC
  AGATCTTTCCAAGCTGTTCAGAACCCTAATGGACGTTCCCACTGTGGGGGACGTTCATCAAGACAGTCTT
  GAAATCGATGAGCTGGAAGATGAACCAATTAAAGAAGGGCCTTCTGATTCCGAAGACACTGTATTTGAAG
  AAACTGACACAGATTTACAAGAGCTTCAGGCCTCAATGGAGCAGCTGCTTAGGGAGCAACCAGGTGACGA
  ATACAGTGAGGAGGAAGAGTCTGTTTTAAAAAGCAGCGATGTGGAGCAGACAGCAAGAGGGACAGATGCC
  CCAGACGAGGAGGACAACCCCAGCAGCGAAAGCGCCCTGAACGAGGAATGGCACTCAGATAATAGTGACG
  CTGAGACCACTAGTGAATGTGAATATGACAGTGTCTTTAACCATTTAGAGGAACTAAGACTTCACTTGGA
  GCAAGAAATGGGCTTTGAAAAGTTCTTTGAGGTTTATGAGAAAGTAAAGGCTATTCATGAGGATGAAGAT
  GAAAATATTGAAATTTGTTCAACAATAGTTGAGAATATTTTGGGCAATGAGCACCAGCATCTCTATGCCA
  AGATTCTGCATTTAGTCATGGCAGATGGAGCCTATCAGGAAGATAATGATGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231703 representing NM_001293638
  Red=Cloning site Green=Tags(s)
  
  MEKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNIVQYKESF
  EENGSLYIVMDYCEGGDLFKRINAQKGALFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDG
  TVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGNMKN
  LVLKIISGSFPPVSPHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTL
  SKFGPQPLPGKRPASGQGVSSFVPAQKITKPAAKYGVPLTYKKYGDKKLLEKKPPPKHKQAHQIPVKKMN
  SGEERKKMSEEAAKKRRLEFIEKEKKQKDQIRFLKAEQMKRQEKQRLERINRAREQGWRNVLRAGGSGEV
  KASFFGIGGAVSPSPCSPRGQYEHYHAIFDQMQRLRAEDNEARWKGGIYGRWLPERQKGHLAVERANQVE
  EFLQRKREAMQNKARAEGHVGLLQNLASLYGGRPSSSRGGKPRNNEEEVYLARLRQIRLQNFNERQQIKA
  KLRGENKEADGTKGQEATEETDMRLKKMESLKAQTNARAAVLKEQLERKRKEAYEREKKVWEEHLVARVK
  SSDVPLPLELLETGGSPSKQQVKPVISVTSALKEVGLDGSLTDTQEEEMEKSNSAISSKREILRRLNENL
  KAQEDEKEKQHHSGSCETVGHKDEREYETENAISSDRKKWEMGGQLVIPLDAVTLDTSFSATEKHTVGEV
  IKLDSNGSPRKVWGKNPTDSVLKILGEAELQLQTELLENTSFKSEVYAEEENYKPLLTEEENLQCISKEI
  NPSATVDSTETKSPKFTEVSPQMSEGNVEEPDDLETEVLQEPSSTHTDGSLPPVLNDVWTREKEAAKETE
  LEDKVAVQQSEVCEDRIPGNVDQSCKDQRDPAVDDSPQSGCDVEKSVQPESIFQKVVHSKDLNLVQAVHC
  SPEEPIPIRSHSDSPPKTKSKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVHQDSL
  EIDELEDEPIKEGPSDSEDTVFEETDTDLQELQASMEQLLREQPGDEYSEEEESVLKSSDVEQTARGTDA
  PDEEDNPSSESALNEEWHSDNSDAETTSECEYDSVFNHLEELRLHLEQEMGFEKFFEVYEKVKAIHEDED
  ENIEICSTIVENILGNEHQHLYAKILHLVMADGAYQEDNDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293638
• ORF Size: 3693 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001293638.1, NP_001280567.1
• RefSeq Size: 5503
• RefSeq ORF: 3696
• Locus ID: 18004
• MW: 140.1 kDa