Kcnma1 (NM_001253365) Mouse Tagged ORF Clone

CAT#: MR231709

Kcnma1 (myc-DDK-tagged) - Mouse potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1), transcript variant 9

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnma1
• Synonyms: 5730414M22Rik; BKCa; MaxiK; mSlo; mSlo1; Slo; Slo1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231709 representing NM_001253365
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAACGGTGGCGGCGGCGGCGGCGGCAGCAGCGGCGGCGGCGGCGGCGGCGGCGGAGGCAGCGGTC
  TTAGAATGAGCAGCAATATCCACGCGAACCATCTCAGCCTAGACGCGTCCTCCTCCTCCTCTTCCTCCTC
  CTCTTCTTCTTCTTCCTCCTCCTCTTCCTCCTCGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTCATC
  ATACCGGTGACCATGGAGGTGCCGTGCGACAGCCGGGGCCAACGCATGTGGTGGGCTTTCTTGGCCTCCT
  CCATGGTGACTTTCTTCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACGCTCAAGTACCTGTGGACCGT
  TTGCTGCCACTGCGGGGGCAAGACGAAGGAGGCCCAGAAGATAAACAATGGCTCCAGCCAGGCAGATGGT
  ACTCTCAAGCCAGTGGACGAAAAAGAGGAGGTGGTGGCAGCCGAGGTCGGCTGGATGACATCTGTGAAGG
  ACTGGGCAGGGGTGATGATATCCGCCCAGACACTGACTGGCAGAGTCCTGGTTGTGTTAGTCTTTGCTCT
  CAGCATTGGTGCCCTCGTAATATACTTCATAGACTCGTCAAACCCAATAGAATCCTGCCAGAATTTCTAC
  AAAGATTTCACATTACAGATCGACATGGCTTTCAACGTGTTCTTCCTCCTCTACTTTGGCTTGCGGTTTA
  TTGCAGCCAACGATAAGCTGTGGTTCTGGCTGGAAGTGAATTCAGTAGTAGATTTCTTCACAGTCCCTCC
  TGTGTTTGTGTCTGTGTACTTAAACAGAAGTTGGCTTGGCTTGAGATTTTTAAGAGCTCTCAGACTGATA
  CAGTTTTCAGAGATTTTGCAGTTTCTGAATATCCTTAAAACAAGTAACTCCATCAAGCTGGTGAATCTGC
  TCTCCATATTTATCAGCACGTGGCTGACTGCAGCTGGATTCATCCACTTGGTGGAGAATTCAGGGGACCC
  ATGGGAAAATTTCCAAAACAACCAGGCACTTACGTACTGGGAATGTGTCTACTTACTCATGGTCACAATG
  TCTACAGTGGGTTATGGGGACGTTTATGCAAAAACCACACTTGGACGCCTCTTCATGGTCTTCTTCATCC
  TCGGGGGACTGGCCATGTTTGCCAGCTACGTCCCTGAAATCATAGAGTTAATAGGAAACCGCAAGAAATA
  CGGGGGCTCCTATAGCGCGGTTAGTGGAAGAAAGCACATTGTAGTCTGTGGACACATTACTCTGGAGAGT
  GTCTCTAACTTCCTGAAGGACTTTCTGCACAAGGACCGGGATGATGTCAACGTGGAGATTGTCTTTCTTC
  ACAACATCTCCCCTAACCTTGAGCTTGAGGCTCTGTTCAAACGGCATTTCACTCAGGTGGAGTTTTATCA
  GGGCTCTGTCCTCAATCCACATGATCTTGCCAGAGTCAAGATAGAGTCAGCAGATGCATGCCTGATCCTT
  GCCAATAAGTATTGCGCTGACCCGGATGCAGAAGATGCCTCCAACATCATGAGAGTGATCTCCATCAAAA
  ACTACCACCCAAAGATCAGGATCATCACTCAGATGCTGCAGTATCACAACAAGGCCCATCTGCTCAACAT
  CCCCAGCTGGAACTGGAAAGAGGGTGATGACGCAATATGCCTTGCAGAGCTCAAGTTGGGTTTCATAGCC
  CAGAGCTGTCTGGCTCAAGGCCTCTCCACAATGCTTGCCAATCTCTTCTCTATGAGGTCATTCATAAAGA
  TTGAGGAAGACACATGGCAGAAATACTACTTGGAAGGAGTCTCCAATGAAATGTACACAGAATATCTCTC
  CAGTGCCTTCGTGGGTCTGTCCTTCCCTACTGTTTGTGAGCTGTGTTTTGTGAAGCTTAAGCTCCTGATG
  ATAGCCATTGAGTACAAGTCTGCCAACAGAGAGAGCCGAATATTAATTAACCCTGGGAACCACCTTAAGA
  TCCAAGAAGGTACTTTAGGATTTTTCATCGCAAGTGATGCCAAAGAAGTTAAAAGGGCATTTTTTTACTG
  CAAGGCCTGTCATGATGACGTCACAGATCCCAAAAGAATTAAAAAATGTGGCTGCAGGCGGCTGATCTAT
  TTTGAAGATGAGCAGCCGCCAACCCTGTCACCAAAAAAAAAACAACGTAATGGGGGCATGAGGAACTCGC
  CCAACACCTCCCCGAAGCTGATGAGGCATGACCCCTTGTTAATTCCTGGCAATGATCAGATTGACAACAT
  GGACTCCAATGTGAAAAAGTACGACTCCACTGGAATGTTTCACTGGTGTGCACCCAAGGAGATTGAGAAA
  GTCATCTTGACTCGAAGTGAAGCTGCCATGACTGTCCTGAGTGGCCATGTCGTAGTCTGCATCTTTGGGG
  ATGTCAGCTCAGCCCTGATTGGCCTCCGGAACCTGGTGATGCCACTTCGTGCTAGCAACTTTCACTATCA
  TGAGCTCAAACACATTGTGTTTGTGGGCTCCATTGAGTACCTCAAGAGGGAGTGGGAAACACTGCACAAC
  TTCCCGAAAGTGTCCATATTGCCTGGTACACCATTAAGTCGGGCTGATTTAAGGGCTGTCAACATCAACC
  TCTGTGACATGTGCGTTATCCTGTCAGCCAATCAGAATAATATTGATGATACTTCGCTTCAGGACAAGGA
  ATGCATCTTGGCGTCACTCAACATCAAATCTATGCAGTTTGATGACAGCATCGGGGTCTTGCAGGCTAAT
  TCCCAAGGATTCACACCTCCTGGAATGGACAGATCATCACCCGACAACAGCCCAGTGCACGGGATGTTAC
  GCCAGCCGTCCATCACAACTGGGGTCAACATTCCCATCATCACGGAACTCGTGAATGATACCAATGTTCA
  GTTTTTGGACCAAGACGATGACGATGACCCTGACACAGAGCTGTACCTCACACAGCCCTTTGCTTGTGGG
  ACAGCATTTGCCGTCAGCGTCCTGGACTCACTCATGAGCGCGACATACTTCAATGACAATATCCTCACCC
  TAATACGGACCCTGGTGACAGGAGGAGCCACACCAGAGCTCGAGGCTCTAATAGCTGAGGAGAATGCACT
  TCGAGGAGGCTACAGCACTCCGCAGACATTGGCCAACAGGGACCGTTGCCGAGTGGCCCAGTTAGCCCTG
  TTAGATGGTCCCTTTGCAGACTTAGGGGATGGTGGTTGTTATGGTGATCTGTTCTGCAAAGCTCTGAAAA
  CATATAATATGCTTTGTTTTGGAATTTACCGGCTGAGAGATGCCCACCTCAGCACCCCCAGCCAGTGTAC
  AAAAAGGTACGTCATCACCAATCCTCCCTACGAGTTTGAGCTCGTACCAACAGACCTGATCTTCTGCCTG
  ATGCAGTTTGACCACAACGCTGGCCAATCCCGGGCCAGTCTGTCTCATTCCTCCCACTCCTCACAGTCGT
  CCAGTAAGAAGAGCTCCTCCGTCCACTCCATCCCGTCCACAGCAAATCGGCCGAACCGGCCCAAGTCCAG
  GGAGTCCCGCGACAAACAGAATGCAACAAGGATGACTAGAATGGGCCAAGCAGAAAAGAAATGGTTTACA
  GATGAGCCGGATAATGCCTATCCCAGAAACATTCAAATCAAGCCCATGAGTACCCACATGGCTAACCAGA
  TCAACCAATATAAATCCACAAGCAGCCTGATCCCTCCAATCAGAGAAGTTGAAGATGAATGT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231709 representing NM_001253365
  Red=Cloning site Green=Tags(s)
  
  MANGGGGGGGSSGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSVHEPKMDALI
  IPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADG
  TLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFY
  KDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLI
  QFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTM
  STVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLES
  VSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLIL
  ANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIA
  QSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLM
  IAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLIY
  FEDEQPPTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAPKEIEK
  VILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHN
  FPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQAN
  SQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACG
  TAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLAL
  LDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCL
  MQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMTRMGQAEKKWFT
  DEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001253365
• ORF Size: 3702 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001253365.1, NP_001240294.1
• RefSeq Size: 4982
• RefSeq ORF: 3705
• Locus ID: 16531
• MW: 137.9 kDa