Taf1 (NM_001290729) Mouse Tagged ORF Clone

CAT#: MR231893

Taf1 (myc-DDK-tagged) - Mouse TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Taf1
• Synonyms: AU015687; B430306D02Rik; Ccg-1; Ccg1; KAT4; N-TAF1; p250; Taf2a; TAFII250
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231893 representing NM_001290729
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCGGGGTGGGCTGGGCTGCTGCAGGACAAGGGTGGCGGTAGCCCTTCCGTCGTCATGTCCGACA
  CAGACAGCGACGAAGAGTCGGCTGGTGGAGGCCCATTTTCTCTAACTGGGTTCCTGTTCGGCAACATCAA
  TGGAGCAGGGCAGTTGGAAGGCGAAAGCGTCTTGGATGACGAGTGCAAGAAGCATCTTGCGGGCTTGGGG
  GCTTTGGGTCTTGGCAGCCTGATCACTGAACTCACAGCAAATGAAGAATTGTCCGGGAGTGATGGTGCCC
  TGGTAAATGATGAAGGATGGATTAGGAGTAGAGAGGATGCTGTGGACTATTCTGACATCAACGAGGTGGC
  AGAAGATGAAAGTAGAAGATACCAGCAGACAATGGGAAGCTTGCAACCCCTTTGCCACACAGATTATGAT
  GAAGATGACTATGATGCTGATTGTGAAGATATTGATTGCAAATTGATGCCTCCTCCACCTCCACCCCCAG
  GACCACTGAAGAAAGAGAAGGACCAAGATGATATTACTGGTGTGTCTGAAGATGGAGAAGGCATCATCTT
  GCCCTCCATCATTGCCCCTTCCTCTTTGGCCTCAGAGAAAGTGGACTTCAGTAGTTCCTCTGACTCAGAA
  TCTGAGATGGGACCTCAGGACGCAGCACAGTCAGAATCTAAGGATGGACAGCTGACCCTACCATTGGCTG
  GGATTATGCAGCATGATGCCACGAAGCTGTTGCCAAGTGTCACAGAACTTTTTCCAGAATTTAGGCCTGG
  AAAGGTGTTACGCTTTTTACGGCTTTTTGGACCGGGGAAGAATGTTCCATCTGTTTGGAGGAGTGCTCGG
  AGAAAGAGGAAAAAGAAGCACCGTGAGCTTATACAGGAAGGGCAGGTCCAGGAAGAGGAGTGCTCAGTGG
  AATTAGAAGTCAACCAGAAATCTCTGTGGAACTATGACTATGCTCCTCCTCCACTTCCAGACCAGTGTCT
  CTCTGATGATGAAATCACAATGATGGCTCCTGTGGAGTCCAAGTTTTCCCAGTCAACTGGAGACACAGAT
  AAAGTAATGGATACCAAGCCAAGAGTGGCCGAATGGCGTTATGGACCTGCCCGGCTGTGGTATGATATGC
  TAGGTGTTCCTGAAGATGGCAGTGGCTTTGATTATGGCTTCAAAATGAAGAAGACTGAACATGAATCTAC
  AATAAAATGCAACATAATGAAGAAATTAAGGAAACTTGAGGAAAACAGTGGCGTCGATCTTCTGGCAGAT
  GAAAACTTCCTAATGGTGACACAGCTGCATTGGGAAGATGATATCATTTGGGATGGGGAGGATGTCAAAC
  ACAAAGGAACAAAACCTCAGCGTGCGAGTTTGGCAGGCTGGCTTCCTTCCAGTATGACTAGGAATGCCAT
  GGCCTACAATGTGCAGCAAGGTTTTACAGCTACATTGGATGATGATAAACCTTGGTACTCCATTTTCCCC
  ATTGATAATGAAGATCTGGTATATGGACGTTGGGAGGACAATATCATTTGGGATGCTCAGAACATGCCTA
  GAATTTTGGAGCCTCCTGTTTTGACACTTGATCCCAATGATGAGAACTTGATTTTGGAGATCCCTGATGA
  GAAGGAAGAAGCTACTTCTAATTCTCCTTCTAAGGAGAATAAGAAAGAATCATCTCTGAAGAAGAGTCGA
  ATTCTCTTAGGAAAAACAGGAGTCATCAAGGAAGAGCCACAGCAGAACATGTCTCAGCCAGAAGTGAAAG
  ATCCATGGAATCTCTCCAATGATGAGTATTACTATCCCAAGCAACAGGGCCTTCGAGGTACTTTTGGAGG
  AAATATTATCCAGCACTCAATTCCAGCTGTAGAATTACGGCAGCCCTTCTTTCCTACCCACATGGGACCC
  ATCAAACTTCGGCAGTTCCATCGCCCACCTCTAAAAAAGTACTCCTTTGGGGCACTATCTCAGCCAGGTC
  CACACTCAGTTCAGCCTTTGCTGAAGCACATCAAAAAGAAGGCAAAGATGAGAGAACAAGAGAGGCAAGC
  CTCGGGTGGTGGAGAGATGTTTTTTATGCGCACACCTCAGGATCTCACAGGCAAAGATGGAGACCTTATT
  CTTGCTGAATACAGTGAGGAAAATGGACCATTAATGATGCAAGTTGGCATGGCAACCAAGATAAAAAACT
  ACTATAAGCGGAAACCTGGAAAAGATCCTGGAGCCCCAGATTGCAAATACGGAGAAACTGTTTATTGCCA
  TACATCGCCTTTCCTGGGTTCTCTTCATCCTGGCCAGTTACTGCAGGCATTTGAGAACAACCTTTTTCGT
  GCTCCAATTTATCTTCATAAGATGCCAGAATCTGACTTCCTGATCATTCGAACAAGGCAGGGTTACTTTA
  TTCGAGAATTAGTGGATATTTTTGTTGTTGGCCAGCAGTGTCCCTTATTTGAAGTTCCTGGTCCTAACTC
  CAAAAGGGCCAATACACACATCCGAGATTTTCTTCAGGTTTTTATTTACCGGCTCTTTTGGAAGAGTAAA
  GATAGGCCACGGAGGATACGGATGGAAGATATAAAGAAAGCCTTTCCTTCACATTCAGAAAGCAGCATCC
  GGAAGAGGCTAAAGCTCTGTGCCGACTTCAAACGTACAGGGATGGATTCAAACTGGTGGGTGCTGAAGTC
  TGATTTTCGTTTACCAACGGAAGAGGAGATCCGAGCTATGGTATCCCCAGAACAGTGCTGTGCTTATTAT
  AGCATGATAGCTGCTGAACAGAGACTAAAGGATGCTGGCTATGGAGAGAAGTCCTTTTTTGCTCCTGAGG
  AAGAAAATGAAGAAGATTTCCAGATGAAGATTGATGATGAAGTTCGTACTGCTCCTTGGAATACTACAAG
  GGCTTTCATTGCTGCCATGAAGGGCAAATGTCTCCTGGAGGTGACTGGAGTAGCAGATCCTACAGGCTGT
  GGTGAAGGGTTCTCCTATGTGAAGATTCCAAACAAACCGACACAACAGAAGGATGATAAAGAGCCTCAGC
  CAGTGAAAAAGACAGTCACAGGAACAGATGCAGACCTTCGTCGCCTGTCTCTGAAAAATGCCAAGCAGCT
  TCTTCGTAAATTTGGTGTGCCTGAGGAGGAGATTAAAAAGTTGTCCCGCTGGGAAGTTATTGATGTGGTA
  CGTACAATGTCAACAGAACAGGCTCGCTCTGGAGAGGGGCCCATGAGTAAATTTGCTCGTGGATCAAGGT
  TTTCAGTGGCTGAGCATCAAGAACGTTACAAAGAGGAATGTCAGCGCATCTTTGACCTACAGAACAAGGT
  TTTGTCATCAACAGAGGTCTTATCAACTGACACAGACAGCAGCTCAGCTGAAGACAGTGACTTTGAAGAA
  ATGGGAAAGAATATTGAGAATATGCTGCAGAACAAGAAAACAAGTTCTCAGCTGTCTCGGGAGCGGGAGG
  AGCAGGAACGGAAGGAACTCCAGCGGATGCTACTGGCAGCAGGCTCAGCAGCAGCAGGAAACAATCACAG
  AGATGATGACACAGCTTCAGTGACCAGCCTTAACTCTTCTGCCACTGGCCGCTGTCTCAAGATTTATCGA
  ACATTTCGAGATGAGGAGGGGAAGGAATATGTTCGTTGTGAGACAGTCCGAAAAGCAACTGTTATTGATG
  CTTATGTACGCATACGGACCACAAAAGATGAAGAGTTCATTCGAAAATTTGCCCTTTTTGATGAGCAACA
  CCGAGAAGAGATGAGAAAAGAACGGCGGAGGATTCAAGAGCAATTGAGGAGACTTAAACGGAACCAGGAA
  AAGGAAAAGCTAAAGGGGCCTCCTGAGAAAAAGCCCAAAAAAATGAAGGAACGGCCTGACTTAAAATTGA
  AATGTGGAGCATGTGGTGCCATCGGGCACATGAGGACAAATAAATTCTGCCCCCTTTATTATCAAACAAA
  TGCTCCACCTTCTAACCCTGTTGCTATGACCGAGGAACAAGAGGAAGAGCTGGAAAAGACAGTCATTCAT
  AATGATAATGAAGAACTTATCAAGGTTGAAGGGACCAAGATCGTTTTGGGCAAACAGCTAATTGAGAGTG
  CAGATGAAGTTCGCAGAAAATCTCTGGTCCTGAAGTTCCCTAAACAACAACTTCCCCCCAAGAAAAAACG
  ACGGGTTGGAACCACTGTTCACTGTGACTATTTGAATAGACCTCATAAATCCATCCACCGGCGCCGGACA
  GACCCGATGGTGACACTGTCTTCTATCTTGGAGTCTATTATCAATGACATGAGAGATCTTCCAAATACAT
  ACCCTTTCCATACTCCAGTTAATGCAAAGGTTGTAAAGGATTACTATAAAATCATCACTCGACCAATGGA
  TCTACAGACACTCCGTGAAAACGTGCGCAAACGCCTTTATCCGTCTCGGGAAGAATTCAGAGAACATTTG
  GAGCTAATTGTGAAAAACAGTGCAACTTATAATGGACCAAAGCACTCACTGACTCAGATTTCTCAATCCA
  TGCTGGATCTCTGCGATGAAAAACTCAAAGAGAAAGAGGATAAGTTGGCACGTTTAGAGAAAGCTATCAA
  CCCCTTGCTTGACGATGATGATCAAGTAGCCTTTTCTTTTATTCTGGATAACATTGTCACTCAGAAAATG
  ATGGCAGTTCCAGATTCTTGGCCATTTCATCACCCAGTTAATAAGAAATTTGTTCCAGATTATTACAAAG
  TGATTGTCAGTCCAATGGATTTGGAGACTATACGTAAGAATATCTCCAAGCACAAATACCAGAGTCGAGA
  GAGCTTTCTAGACGACGTAAACCTGATTCTGGCCAACAGTGTTAAGTACAATGGGCCTGAGAGTCAGTAT
  ACTAAGACTGCTCAGGAGATTGTGAACGTTTGTCACCAGACATTAACTGAGTATGATGAACATTTGACTC
  AACTTGAGAAAGATATTTGTACAGCTAAAGAAGCAGCTCTGGAGGAAGCAGAATTAGAAAGTCTGGACCC
  AATGACTCCAGGGCCATATACACCTCAGGCTAAGCCTCCTGATTTGTATGATAACAACACATCCCTCAGT
  GTTTCTCGAGATGCTTCTGTATATCAAGATGAGAGCAATCTGTCTGTCTTGGATATTCCCAGTGCTACTT
  CAGAAAAGCAGCTAACACAGGAAGGTGGAGATGGAGATGGTGATCTTGCAGATGAAGAGGAAGGAACTGT
  ACAACAGCCCCAAGCCAGTGTCTTGTATGAGGATTTGCTTATGTCTGAAGGCGAAGATGATGAGGAAGAT
  GCTGGGAGTGACGAAGAAGGAGATAACCCTTTCTTTGCTATTCAGCTAAGTGAAAGTGGAAGTGACTCTG
  ATGTGGAATCTGGCAGTTTAAGACCCAAACAGCCCCGTGTGCTTCAAGAGAATACAAGGATGGGCATGGA
  AAATGAAGAAAGCATGATGTCCTATGAGGGAGATGGTGGGGACGCTTCCCGTGGTTTGGAGGATAGCAAC
  ATCAGTTATGGGAGCTATGAGGAGCCTGATCCCAAGTCCAACACCCAGGATACCAGCTTCAGCAGCATCG
  GTGGGTATGAGGTATCAGAGGAGGAAGAAGATGAGGAAGAGCAGCGCTCTGGGCCCAGCGTACTAAGCCA
  GGTCCACCTCTCAGAGGACGAGGAGGACAGTGAGGATTTCCACTCCATTGCCGGAGACACTGATTTGGAC
  TCTGATGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231893 representing NM_001290729
  Red=Cloning site Green=Tags(s)
  
  MGPGWAGLLQDKGGGSPSVVMSDTDSDEESAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLG
  ALGLGSLITELTANEELSGSDGALVNDEGWIRSREDAVDYSDINEVAEDESRRYQQTMGSLQPLCHTDYD
  EDDYDADCEDIDCKLMPPPPPPPGPLKKEKDQDDITGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSE
  SEMGPQDAAQSESKDGQLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSAR
  RKRKKKHRELIQEGQVQEEECSVELEVNQKSLWNYDYAPPPLPDQCLSDDEITMMAPVESKFSQSTGDTD
  KVMDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKMKKTEHESTIKCNIMKKLRKLEENSGVDLLAD
  ENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFTATLDDDKPWYSIFP
  IDNEDLVYGRWEDNIIWDAQNMPRILEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSR
  ILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGP
  IKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLI
  LAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFR
  APIYLHKMPESDFLIIRTRQGYFIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSK
  DRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYY
  SMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGC
  GEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVV
  RTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEVLSTDTDSSSAEDSDFEE
  MGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAAAGNNHRDDDTASVTSLNSSATGRCLKIYR
  TFRDEEGKEYVRCETVRKATVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQE
  KEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIH
  NDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRT
  DPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHL
  ELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKM
  MAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQY
  TKTAQEIVNVCHQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQAKPPDLYDNNTSLS
  VSRDASVYQDESNLSVLDIPSATSEKQLTQEGGDGDGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEED
  AGSDEEGDNPFFAIQLSESGSDSDVESGSLRPKQPRVLQENTRMGMENEESMMSYEGDGGDASRGLEDSN
  ISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDTDLD
  SDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290729
• ORF Size: 5679 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001290729.1, NP_001277658.1
• RefSeq Size: 8033
• RefSeq ORF: 5682
• Locus ID: 270627
• MW: 215.1 kDa