SKIP (INPP5K) (NM_016532) Human Tagged ORF Clone

CAT#: RC200092

INPP5K (Myc-DDK-tagged)-Human inositol polyphosphate-5-phosphatase K (INPP5K), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY402561) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200092 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified INPP5K protein (Cat# TP300092). The protein was produced from HEK293T cells transfected with INPP5K cDNA clone (Cat# RC200092) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: INPP5K
• Synonyms: MDCCAID; PPS; SKIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200092 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCGCGGAAGCTGAGCGGGCCGAAAGGCAGGAGGCTCAGCATACACGTCGTGACTTGGAACGTGG
  CTTCGGCAGCGCCCCCTCTAGATCTCAGTGACCTGCTTCAGCTGAACAACCGGAACCTCAATCTTGACAT
  ATATGTTATTGGTTTGCAGGAATTGAACTCTGGGATCATAAGCCTCCTTTCCGATGCTGCCTTTAATGAC
  TCGTGGAGCAGTTTCCTCATGGATGTGCTTTCCCCTCTGAGCTTCATCAAGGTCTCCCATGTCCGTATGC
  AGGGGATCCTCTTACTGGTCTTTGCCAAGTATCAGCATTTGCCCTATATCCAGATTCTGTCTACTAAATC
  CACCCCCACTGGCCTGTTTGGGTACTGGGGGAACAAAGGTGGAGTCAACATCTGCCTGAAGCTTTATGGC
  TACTATGTCAGCATCATCAACTGCCACCTGCCTCCCCACATTTCCAACAATTACCAGCGGCTGGAGCACT
  TTGACCGGATCCTGGAGATGCAGAATTGTGAGGGGCGAGACATCCCAAACATCCTGGACCACGACCTCAT
  TATCTGGTTTGGAGACATGAACTTTCGGATCGAGGACTTTGGGTTGCACTTTGTTCGGGAATCCATTAAA
  AATCGGTGCTACGGTGGCCTGTGGGAGAAGGACCAGCTCAGCATTGCCAAGAAACATGACCCGCTGCTCC
  GGGAGTTCCAGGAGGGCCGCCTACTCTTCCCGCCCACCTACAAGTTTGATAGGAACTCCAACGACTATGA
  CACCAGTGAGAAAAAACGCAAGCCTGCATGGACCGATCGCATCCTGTGGAGGCTGAAGCGGCAGCCCTGT
  GCTGGCCCCGACACTCCCATACCGCCGGCGTCACACTTCTCCTTGTCTCTGAGGGGCTACAGCAGCCACA
  TGACGTACGGCATCAGCGACCACAAGCCTGTCTCCGGCACGTTCGACTTGGAGCTGAAGCCATTGGTGTC
  TGCTCCGCTGATCGTCCTGATGCCCGAGGACCTGTGGACCGTGGAAAATGACATGATGGTCAGCTACTCT
  TCAACCTCGGACTTCCCCAGCAGCCCGTGGGACTGGATTGGACTGTACAAGGTGGGGCTGCGGGACGTTA
  ATGACTACGTGTCCTATGCCTGGGTCGGGGACAGCAAGGTCTCCTGCAGCGACAACCTGAACCAGGTTTA
  CATCGACATCAGCAATATCCCTACCACTGAAGATGAGTTTCTCCTCTGTTACTACAGCAACAGTCTGCGT
  TCTGTGGTGGGGATAAGCAGACCCTTCCAGATCCCGCCTGGCTCCTTGAGGGAGGACCCACTGGGTGAAG
  CACAGCCACAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200092 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSRKLSGPKGRRLSIHVVTWNVASAAPPLDLSDLLQLNNRNLNLDIYVIGLQELNSGIISLLSDAAFND
  SWSSFLMDVLSPLSFIKVSHVRMQGILLLVFAKYQHLPYIQILSTKSTPTGLFGYWGNKGGVNICLKLYG
  YYVSIINCHLPPHISNNYQRLEHFDRILEMQNCEGRDIPNILDHDLIIWFGDMNFRIEDFGLHFVRESIK
  NRCYGGLWEKDQLSIAKKHDPLLREFQEGRLLFPPTYKFDRNSNDYDTSEKKRKPAWTDRILWRLKRQPC
  AGPDTPIPPASHFSLSLRGYSSHMTYGISDHKPVSGTFDLELKPLVSAPLIVLMPEDLWTVENDMMVSYS
  STSDFPSSPWDWIGLYKVGLRDVNDYVSYAWVGDSKVSCSDNLNQVYIDISNIPTTEDEFLLCYYSNSLR
  SVVGISRPFQIPPGSLREDPLGEAQPQI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016532
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016532.1, NM_016532.2, NM_016532.3, NP_057616.2
• RefSeq Size: 3001 bp
• RefSeq ORF: 1347 bp
• Locus ID: 51763
• Domains: IPPc, Exo_endo_phos
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Inositol phosphate metabolism, Insulin signaling pathway, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 51.1 kDa
• Gene Summary: This gene encodes a protein with 5-phosphatase activity toward polyphosphate inositol. The protein localizes to the cytosol in regions lacking actin stress fibers. It is thought that this protein may negatively regulate the actin cytoskeleton. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]