NUDT5 (NM_014142) Human Tagged ORF Clone
CAT#: RC200204
NUDT5 (Myc-DDK-tagged)-Human nudix (nucleoside diphosphate linked moiety X)-type motif 5 (NUDT5)
"NM_014142" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | NUDT5 |
Synonyms | hNUDT5; YSA1; YSA1H; YSAH1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200204 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGCCAAGAACCAACGGAATCTTCTCAGAATGGCAAACAGTATATCATTTCAGAGGAGTTAATTT CAGAAGGAAAATGGGTCAAGCTTGAAAAAACAACGTACATGGATCCTACTGGTAAAACTAGAACTTGGGA ATCAGTGAAACGTACAACCAGGAAAGAGCAGACTGCGGATGGTGTCGCGGTCATCCCCGTGCTGCAGAGA ACACTTCACTATGAGTGTATCGTTCTGGTGAAACAGTTCCGACCACCAATGGGGGGCTACTGCATAGAGT TCCCTGCAGGTCTCATAGATGATGGTGAAACCCCAGAAGCAGCTGCTCTCCGGGAGCTTGAAGAAGAAAC TGGCTACAAAGGGGACATTGCCGAATGTTCTCCAGCGGTCTGTATGGACCCAGGCTTGTCAAACTGTACT ATACACATCGTGACAGTCACCATTAACGGAGATGATGCCGAAAACGCAAGGCCGAAGCCAAAGCCAGGGG ATGGAGAGTTTGTGGAAGTCATTTCTTTACCCAAGAATGACCTGCTGCAGAGACTTGATGCTCTGGTAGC TGAAGAACATCTCACAGTGGACGCCAGGGTCTATTCCTACGCTCTAGCACTGAAACATGCAAATGCAAAG CCATTTGAAGTGCCCTTCTTGAAATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200204 protein sequence
Red=Cloning site Green=Tags(s) MESQEPTESSQNGKQYIISEELISEGKWVKLEKTTYMDPTGKTRTWESVKRTTRKEQTADGVAVIPVLQR TLHYECIVLVKQFRPPMGGYCIEFPAGLIDDGETPEAAALRELEEETGYKGDIAECSPAVCMDPGLSNCT IHIVTVTINGDDAENARPKPKPGDGEFVEVISLPKNDLLQRLDALVAEEHLTVDARVYSYALALKHANAK PFEVPFLKF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_014142 |
ORF Size | 657 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014142.1, NM_014142.2, NM_014142.3, NP_054861.2 |
RefSeq Size | 1224 bp |
RefSeq ORF | 660 bp |
Locus ID | 11164 |
Domains | NUDIX |
Protein Pathways | Purine metabolism |
MW | 24.3 kDa |
Gene Summary | This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates, including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and 8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in the inhibition of ATP-activated potassium channels. 8-oxoGua is an oxidized form of guanine that can potentially alter genetic information by pairing with adenine and cytosine in RNA. Presence of 8-oxoGua in RNA results in formation of abnormal proteins due to translational errors. [provided by RefSeq, Aug 2013] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC319410 | NUDT5 (untagged)-Human nudix (nucleoside diphosphate linked moiety X)-type motif 5 (NUDT5) |
USD 310.00 |
|
RG200204 | NUDT5 (GFP-tagged) - Human nudix (nucleoside diphosphate linked moiety X)-type motif 5 (NUDT5) |
USD 460.00 |
|
RC200204L1 | Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 5 (NUDT5), Myc-DDK-tagged |
USD 768.00 |
|
RC200204L2 | Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 5 (NUDT5), mGFP tagged |
USD 620.00 |
|
RC200204L3 | Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 5 (NUDT5), Myc-DDK-tagged |
USD 620.00 |
|
RC200204L4 | Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 5 (NUDT5), mGFP tagged |
USD 620.00 |
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