WBP11 (NM_016312) Human Tagged ORF Clone

CAT#: RC200214

WBP11 (Myc-DDK-tagged)-Human WW domain binding protein 11 (WBP11)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WBP11
• Synonyms: NPWBP; PPP1R165; SIPP1; WBP-11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200214 representing NM_016312
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACGGAGATCTACATCATCCACCAAGAGTGGAAAATTTATGAACCCCACAGACCAAGCCCGAAAGG
  AAGCCCGGAAGAGAGAATTAAAGAAGAACAAAAAACAGCGCATGATGGTTCGAGCTGCAGTTTTAAAGAT
  GAAGGATCCAAAACAGATAATCCGAGACATGGAGAAATTGGATGAAATGGAGTTTAACCCAGTGCAACAG
  CCACAATTAAATGAGAAAGTACTGAAAGACAAGCGTAAAAAGCTGCGTGAAACCTTTGAACGTATTCTAC
  GACTCTATGAAAAAGAGAATCCAGATATTTACAAAGAATTGAGAAAGCTAGAAGTAGAATATGAACAGAA
  GAGGGCTCAACTTAGCCAATATTTTGATGCTGTCAAGAATGCTCAGCATGTGGAAGTGGAGAGTATTCCT
  TTGCCAGATATGCCACATGCTCCTTCCAACATTTTGATCCAGGACATTCCACTTCCTGGTGCCCAGCCAC
  CCTCTATCCTAAAGAAAACCTCAGCCTATGGACCTCCAACTCGGGCAGTTTCTATCCTTCCTCTTCTTGG
  ACATGGTGTTCCACGTTTGCCCCCTGGCAGAAAACCTCCTGGCCCTCCCCCTGGTCCACCTCCTCCTCAA
  GTCGTGCAGATGTATGGCCGTAAAGTGGGTTTTGCCCTAGATCTTCCCCCTCGTAGGCGAGATGAAGACA
  TGTTATATAGTCCTGAACTTGCCCAGCGAGGTCATGATGATGATGTTTCTAGCACCAGTGAAGATGATGG
  CTATCCTGAGGACATGGATCAAGATAAGCATGATGACAGTACTGATGACAGTGACACCGACAAATCAGAT
  GGAGAAAGTGACGGGGATGAATTTGTGCACCGTGATAATGGTGAGAGAGACAACAATGAAGAAAAGAAGT
  CAGGTCTGAGTGTACGGTTTGCAGATATGCCTGGAAAATCAAGGAAGAAAAAGAAGAACATGAAGGAACT
  GACTCCTCTTCAAGCCATGATGCTTCGTATGGCAGGTCAAGAAATCCCTGAGGAGGGACGGGAAGTAGAG
  GAATTTTCAGAGGACGATGATGAAGATGATTCTGATGACTCTGAAGCAGAAAAGCAATCACAAAAGCAGC
  ATAAAGAGGAATCCCATTCTGATGGCACATCCACTGCTTCTTCACAGCAGCAGGCTCCGCCGCAGTCTGT
  TCCTCCTTCTCAGATACAAGCACCTCCCATGCCAGGACCACCACCTCTTGGACCACCACCTGCTCCACCA
  TTACGGCCTCCTGGGCCACCTACAGGCCTTCCTCCTGGTCCACCTCCAGGAGCTCCTCCATTCCTGAGAC
  CACCTGGAATGCCAGGACTCCGAGGGCCCTTACCCCGACTTTTACCTCCAGGACCACCACCAGGCCGACC
  CCCTGGCCCTCCCCCAGGTCCACCTCCAGGTCTGCCTCCTGGTCCCCCTCCTCGTGGACCCCCACCAAGG
  CTACCTCCCCCTGCACCTCCAGGTATTCCTCCACCTCGTCCTGGCATGATGCGCCCACCTTTGGTGCCTC
  CCCTTGGACCTGCCCCCCCTGGGCTGTTCCCACCAGCTCCCTTGCCAAACCCTGGGGTTTTAAGTGCCCC
  ACCCAACTTGATTCAGCGACCCAAGGCGGATGATACAAGTGCAGCCACCATTGAGAAGAAAGCCACAGCA
  ACCATCAGTGCCAAGCCACAGATCACTAATCCCAAGGCAGAGATTACTCGATTTGTGCCCACTGCACTGA
  GAGTACGTCGGGAGAATAAAGGGGCTACTGCTGCTCCCCAAAGAAAGTCAGAGGATGATTCTGCTGTGCC
  TCTTGCCAAAGCAGCACCCAAATCTGGTCCTTCTGTTCCTGTCTCAGTACAAACTAAGGATGATGTCTAT
  GAGGCTTTCATGAAAGAGATGGAAGGGCTACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200214 representing NM_016312
  Red=Cloning site Green=Tags(s)
  
  MGRRSTSSTKSGKFMNPTDQARKEARKRELKKNKKQRMMVRAAVLKMKDPKQIIRDMEKLDEMEFNPVQQ
  PQLNEKVLKDKRKKLRETFERILRLYEKENPDIYKELRKLEVEYEQKRAQLSQYFDAVKNAQHVEVESIP
  LPDMPHAPSNILIQDIPLPGAQPPSILKKTSAYGPPTRAVSILPLLGHGVPRLPPGRKPPGPPPGPPPPQ
  VVQMYGRKVGFALDLPPRRRDEDMLYSPELAQRGHDDDVSSTSEDDGYPEDMDQDKHDDSTDDSDTDKSD
  GESDGDEFVHRDNGERDNNEEKKSGLSVRFADMPGKSRKKKKNMKELTPLQAMMLRMAGQEIPEEGREVE
  EFSEDDDEDDSDDSEAEKQSQKQHKEESHSDGTSTASSQQQAPPQSVPPSQIQAPPMPGPPPLGPPPAPP
  LRPPGPPTGLPPGPPPGAPPFLRPPGMPGLRGPLPRLLPPGPPPGRPPGPPPGPPPGLPPGPPPRGPPPR
  LPPPAPPGIPPPRPGMMRPPLVPPLGPAPPGLFPPAPLPNPGVLSAPPNLIQRPKADDTSAATIEKKATA
  TISAKPQITNPKAEITRFVPTALRVRRENKGATAAPQRKSEDDSAVPLAKAAPKSGPSVPVSVQTKDDVY
  EAFMKEMEGLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016312
• ORF Size: 1923 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016312.1, NM_016312.2, NP_057396.1
• RefSeq Size: 2690
• RefSeq ORF: 1926
• Locus ID: 51729
• Protein Pathways: Spliceosome
• MW: 69.8 kDa
• Gene Summary: This gene encodes a nuclear protein, which colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. This protein has 95% amino acid sequence identity to the mouse Wbp11 protein. It contains two proline-rich regions that bind to the WW domain of Npw38, a nuclear protein, and thus this protein is also called Npw38-binding protein NpwBP. The Npw38-NpwBP complex may function as a component of an mRNA factory in the nucleus. [provided by RefSeq, Jul 2008]