MRG15 (MORF4L1) (NM_006791) Human Tagged ORF Clone

CAT#: RC200251

MORF4L1 (Myc-DDK-tagged)-Human mortality factor 4 like 1 (MORF4L1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY402027) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200251 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MORF4L1 protein (Cat# TP300251). The protein was produced from HEK293T cells transfected with MORF4L1 cDNA clone (Cat# RC200251) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MORF4L1
• Synonyms: Eaf3; FWP006; HsT17725; MEAF3; MORFRG15; MRG15; S863-6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200251 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGAAGCAGGACCCGAAGCCTAAATTCCAGGAGGGTGAGCGAGTGCTGTGCTTTCATGGGCCTC
  TTCTTTATGAAGCAAAGTGTGTAAAGGTTGCCATAAAGGACAAACAAGTGAAATACTTCATACATTACAG
  TGGTTGGAATAAAAATTGGGATGAATGGGTTCCGGAGAGCAGAGTACTCAAATACGTGGACACCAATTTG
  CAGAAACAGCGAGAACTTCAAAAAGCCAATCAGGAGCAGTATGCAGAGGGGAAGATGAGAGGGGCTGCCC
  CAGGAAAGAAGACATCTGGTCTGCAACAGAAAAATGTTGAAGTGAAAACGAAAAAGAACAAACAGAAAAC
  ACCTGGAAATGGAGATGGTGGCAGTACCAGTGAGACCCCTCAGCCTCCTCGGAAGAAAAGGGCCCGGGTA
  GATCCTACTGTTGAAAATGAGGAAACATTCATGAACAGAGTTGAAGTTAAAGTAAAGATTCCTGAAGAGC
  TAAAACCGTGGCTTGTTGATGACTGGGACTTAATTACCAGGCAAAAACAGCTCTTTTATCTTCCTGCCAA
  GAAGAATGTGGATTCCATTCTTGAGGATTATGCAAATTACAAGAAATCTCGTGGAAACACAGATAATAAG
  GAGTATGCGGTTAATGAAGTTGTGGCAGGGATAAAAGAATACTTCAACGTAATGTTGGGTACCCAGCTAC
  TCTATAAATTTGAGAGACCACAGTATGCTGAAATTCTTGCAGATCATCCCGATGCACCCATGTCCCAGGT
  GTATGGAGCGCCACATCTCCTGAGATTATTTGTACGAATTGGAGCAATGTTGGCTTATACACCTCTGGAT
  GAGAAGAGCCTTGCTTTATTACTCAATTATCTTCACGATTTCCTAAAGTACCTGGCAAAGAATTCTGCAA
  CTTTGTTCAGTGCCAGCGATTATGAAGTGGCTCCTCCTGAGTACCATCGGAAAGCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200251 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPKQDPKPKFQEGERVLCFHGPLLYEAKCVKVAIKDKQVKYFIHYSGWNKNWDEWVPESRVLKYVDTNL
  QKQRELQKANQEQYAEGKMRGAAPGKKTSGLQQKNVEVKTKKNKQKTPGNGDGGSTSETPQPPRKKRARV
  DPTVENEETFMNRVEVKVKIPEELKPWLVDDWDLITRQKQLFYLPAKKNVDSILEDYANYKKSRGNTDNK
  EYAVNEVVAGIKEYFNVMLGTQLLYKFERPQYAEILADHPDAPMSQVYGAPHLLRLFVRIGAMLAYTPLD
  EKSLALLLNYLHDFLKYLAKNSATLFSASDYEVAPPEYHRKAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006791
• ORF Size: 969 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006791.1, NM_006791.2, NM_006791.3, NP_006782.1
• RefSeq Size: 1894 bp
• RefSeq ORF: 972 bp
• Locus ID: 10933
• Domains: CHROMO
• Protein Families: Transcription Factors
• MW: 37.2 kDa
• Gene Summary: Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also component of the mSin3A complex which acts to repress transcription by deacetylation of nucleosomal histones. Required for homologous recombination repair (HRR) and resistance to mitomycin C (MMC). Involved in the localization of PALB2, BRCA2 and RAD51, but not BRCA1, to DNA-damage foci. [UniProtKB/Swiss-Prot Function]