Lysyl tRNA synthetase (KARS) (NM_005548) Human Tagged ORF Clone
CAT#: RC200311L3
- LentiORF®
Lenti ORF clone of Human lysyl-tRNA synthetase (KARS), transcript variant 2, Myc-DDK-tagged
"NM_005548" in other vectors (6)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | KARS |
Synonyms | CMTRIB; DFNB89; KARS1; KARS2; KRS |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC200311).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_005548 |
ORF Size | 1791 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005548.1, NP_005539.1 |
RefSeq Size | 2017 bp |
RefSeq ORF | 1794 bp |
Locus ID | 3735 |
Cytogenetics | 16q23.1 |
Domains | tRNA-synt_2, tRNA_anti |
Protein Pathways | Aminoacyl-tRNA biosynthesis |
MW | 68 kDa |
Gene Summary | 'Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC116645 | KARS (untagged)-Human lysyl-tRNA synthetase (KARS), transcript variant 2 |
USD 310.00 |
|
RC200311 | KARS (Myc-DDK-tagged)-Human lysyl-tRNA synthetase (KARS), transcript variant 2 |
USD 98.00 |
|
RG200311 | KARS (GFP-tagged) - Human lysyl-tRNA synthetase (KARS), transcript variant 2 |
USD 460.00 |
|
RC200311L1 | Lenti ORF clone of Human lysyl-tRNA synthetase (KARS), transcript variant 2, Myc-DDK-tagged |
USD 768.00 |
|
RC200311L2 | Lenti ORF clone of Human lysyl-tRNA synthetase (KARS), transcript variant 2, mGFP tagged |
USD 620.00 |
|
RC200311L4 | Lenti ORF clone of Human lysyl-tRNA synthetase (KARS), transcript variant 2, mGFP tagged |
USD 620.00 |
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