APEX2 (NM_014481) Human Tagged ORF Clone

CAT#: RC200320

APEX2 (Myc-DDK-tagged)-Human APEX nuclease (apurinic/apyrimidinic endonuclease) 2 (APEX2), nuclear gene encoding mitochondrial protein

Product Images

Western blot validation of overexpression lysate (Cat# LY415240) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200320 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified APEX2 protein (Cat# TP300320). The protein was produced from HEK293T cells transfected with APEX2 cDNA clone (Cat# RC200320) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: APEX2
• Synonyms: APE2; APEXL2; XTH2; ZGRF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200320 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCGCGTGGTGAGCTGGAACATCAATGGGATTCGGAGACCCCTGCAAGGGGTGGCAAATCAGGAAC
  CCAGCAACTGTGCCGCCGTGGCCGTGGGGCGCATTTTGGACGAGCTGGATGCGGATATCGTCTGTCTCCA
  GGAAACCAAAGTGACCAGGGATGCACTGACAGAGCCCCTGGCTATCGTTGAGGGTTATAACTCCTATTTC
  AGCTTCAGCCGCAACCGTAGCGGCTATTCTGGTGTAGCCACCTTCTGTAAGGACAATGCTACCCCAGTGG
  CTGCTGAAGAAGGCCTGAGTGGCCTGTTTGCCACCCAGAATGGGGATGTTGGTTGCTATGGAAACATGGA
  TGAGTTTACCCAAGAGGAACTCCGGGCTCTGGATAGTGAGGGCAGGGCCCTCCTCACACAGCATAAGATC
  CGCACATGGGAAGGTAAGGAGAAGACCTTGACCCTAATCAACGTGTACTGCCCCCATGCGGACCCTGGGA
  GGCCTGAGCGGCTAGTCTTTAAGATGCGCTTCTATCGTTTGCTGCAAATCCGAGCAGAAGCCCTCCTGGC
  GGCAGGCAGCCATGTGATCATTCTGGGTGACCTGAATACAGCCCACCGCCCCATTGACCACTGGGATGCA
  GTCAACCTGGAATGCTTTGAAGAGGACCCAGGGCGCAAGTGGATGGACAGCTTGCTCAGTAACTTGGGGT
  GCCAGTCTGCCTCTCATGTAGGGCCCTTCATCGATAGCTACCGCTGCTTCCAACCAAAGCAGGAGGGGGC
  CTTCACCTGCTGGTCAGCAGTCACTGGCGCCCGCCATCTCAACTATGGCTCCCGGCTTGACTATGTGCTG
  GGGGACAGGACCCTGGTCATAGACACCTTTCAGGCCTCTTTCCTGCTGCCTGAGGTGATGGGCTCTGACC
  ACTGCCCTGTGGGTGCAGTCTTGAGTGTGTCCTCTGTGCCTGCAAAACAGTGCCCACCTCTGTGCACCCG
  CTTCCTCCCTGAGTTTGCAGGCACCCAGCTCAAGATCCTTCGCTTCCTAGTTCCTCTCGAACAAAGTCCT
  GTGTTGGAGCAGTCGACGCTGCAGCACAACAATCAAACCCGGGTACAGACATGCCAAAACAAAGCCCAAG
  TGCGCTCAACCAGGCCTCAGCCCAGTCAGGTTGGCTCTAGCAGAGGCCAGAAAAACCTGAAGAGCTACTT
  TCAGCCCTCCCCTAGCTGTCCCCAAGCCTCTCCTGACATAGAGCTGCCTAGCCTACCACTGATGAGCGCC
  CTCATGACCCCGAAGACTCCAGAAGAGAAGGCAGTGGCCAAAGTGGTGAAGGGGCAGGCCAAGACTTCAG
  AAGCCAAAGATGAGAAGGAGTTACGGACCTCATTCTGGAAGTCTGTGCTGGCGGGGCCCTTGCGCACACC
  CCTCTGTGGGGGCCACAGGGAGCCATGTGTGATGCGTACTGTGAAGAAGCCAGGACCCAACTTGGGCCGC
  CGCTTCTACATGTGTGCCAGGCCCCGGGGTCCTCCCACTGACCCCTCCTCCCGGTGCAACTTCTTCCTCT
  GGAGCAGGCCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200320 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLRVVSWNINGIRRPLQGVANQEPSNCAAVAVGRILDELDADIVCLQETKVTRDALTEPLAIVEGYNSYF
  SFSRNRSGYSGVATFCKDNATPVAAEEGLSGLFATQNGDVGCYGNMDEFTQEELRALDSEGRALLTQHKI
  RTWEGKEKTLTLINVYCPHADPGRPERLVFKMRFYRLLQIRAEALLAAGSHVIILGDLNTAHRPIDHWDA
  VNLECFEEDPGRKWMDSLLSNLGCQSASHVGPFIDSYRCFQPKQEGAFTCWSAVTGARHLNYGSRLDYVL
  GDRTLVIDTFQASFLLPEVMGSDHCPVGAVLSVSSVPAKQCPPLCTRFLPEFAGTQLKILRFLVPLEQSP
  VLEQSTLQHNNQTRVQTCQNKAQVRSTRPQPSQVGSSRGQKNLKSYFQPSPSCPQASPDIELPSLPLMSA
  LMTPKTPEEKAVAKVVKGQAKTSEAKDEKELRTSFWKSVLAGPLRTPLCGGHREPCVMRTVKKPGPNLGR
  RFYMCARPRGPPTDPSSRCNFFLWSRPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014481
• ORF Size: 1554 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014481.1, NM_014481.2, NM_014481.3, NP_055296.2
• RefSeq Size: 2095
• RefSeq ORF: 1557
• Locus ID: 27301
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• MW: 57.4 kDa
• Gene Summary: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]