ZNF174 (NM_001032292) Human Tagged ORF Clone

CAT#: RC200400

ZNF174 (Myc-DDK-tagged)-Human zinc finger protein 174 (ZNF174), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY422302) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200400 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ZNF174 protein (Cat# TP300400). The protein was produced from HEK293T cells transfected with ZNF174 cDNA clone (Cat# RC200400) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF174
• Synonyms: ZSCAN8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200400 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCTAAAATGGAGATAACTTTAAGCTCCAACACTGAAGCTTCCTCCAAGCAAGAGAGACACATAA
  TAGCCAAACTAGAAGAGAAACGGGGCCCTCCTCTGCAAAAAAACTGCCCAGATCCTGAGCTCTGCCGCCA
  GAGCTTCAGACGCTTTTGTTATCAAGAGGTGTCTGGACCCCAAGAGGCGCTCTCCCAGCTCCGACAGCTC
  TGCCGTCAGTGGTTGCAACCCGAGCTGCACACCAAGGAGCAGATTTTGGAGCTTCTGGTGATGGAGCAGT
  TCCTGACCATCCTGCCCCCGGAGATCCAGGCTCGGGTCAGGCATCGATGTCCAATGAGCAGCAAGGAGAT
  TGTGACCCTCGTGGAAGATTTTCACAGAGCATCCAAGAAACCAAAGCAGTGGGTGGCCGTTTGTATGCAG
  GGGCAAAAGGTGCTCTTGGAGAAAACTGGATCTCAGCTTGGAGAACAGGAACTGCCAGACTTTCAACCGC
  AGACTCCTAGGAGAGATCTCAGGGAGAGCTCTCCAGCAGAGCCTTCCCAGGCAGGAGCTTATGACCGGCT
  GAGCCCCCATCATTGGGAGAAATCCCCACTCCTCCAAGAACCAACCCCCAAATTGGCTGGGACAGAACTT
  CTTATAGAAAAGACAGATCCAAATATGGCCACAGATGAACTTCCATGCAAGCTATGGCTGAGTTTCATTG
  CT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200400 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAKMEITLSSNTEASSKQERHIIAKLEEKRGPPLQKNCPDPELCRQSFRRFCYQEVSGPQEALSQLRQL
  CRQWLQPELHTKEQILELLVMEQFLTILPPEIQARVRHRCPMSSKEIVTLVEDFHRASKKPKQWVAVCMQ
  GQKVLLEKTGSQLGEQELPDFQPQTPRRDLRESSPAEPSQAGAYDRLSPHHWEKSPLLQEPTPKLAGTEL
  LIEKTDPNMATDELPCKLWLSFIA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001032292
• ORF Size: 702 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001032292.1, NM_001032292.2, NP_001027463.1
• RefSeq Size: 1593 bp
• RefSeq ORF: 705 bp
• Locus ID: 7727
• Cytogenetics: 16p13.3
• Protein Families: Transcription Factors
• MW: 26.9 kDa
• Gene Summary: 'This gene encodes a protein with three Cys2-His2-type zinc fingers in the carboxy-terminus, a putative nuclear localization signal, and an amino-terminus SCAN box which forms homodimers. This protein is believed to function as a transcriptional repressor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Dec 2016]'