MVD (NM_002461) Human Tagged ORF Clone

CAT#: RC200451

MVD (Myc-DDK-tagged)-Human mevalonate (diphospho) decarboxylase (MVD)

Product Images

Western blot validation of overexpression lysate (Cat# LY419305) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200451 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MVD protein (Cat# TP300451). The protein was produced from HEK293T cells transfected with MVD cDNA clone (Cat# RC200451) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MVD
• Synonyms: FP17780; MDDase; MPD; POROK7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200451 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGGAGAAGCCGCTGGCGGCAGTCACTTGTACAGCGCCGGTCAACATCGCGGTCATCAAGTACT
  GGGGCAAGCGCGATGAAGAGCTGGTTCTGCCCATCAACTCCTCCCTGAGCGTCACTCTGCACCAGGACCA
  GTTAAAAACCACCACAACAGCCGTCATCAGCAAGGACTTCACCGAGGACCGGATTTGGCTGAATGGCCGG
  GAGGAGGATGTGGGGCAGCCGAGGCTGCAGGCCTGCCTGCGGGAGATCCGCTGCCTGGCCCGGAAGCGGA
  GGAACTCACGGGATGGGGACCCGCTGCCCTCCAGCCTCAGCTGCAAGGTGCACGTGGCATCGGTGAACAA
  CTTCCCCACGGCTGCGGGCCTGGCCTCCTCAGCGGCGGGCTATGCCTGCCTAGCCTACACCCTGGCCCGT
  GTCTACGGCGTGGAGAGTGACCTCTCAGAAGTGGCTCGCCGGGGCTCAGGCAGCGCCTGCCGGAGCCTGT
  ATGGGGGCTTTGTGGAGTGGCAGATGGGAGAGCAGGCCGACGGGAAGGACAGCATCGCTCGGCAAGTGGC
  CCCCGAGTCACACTGGCCTGAACTCCGCGTGCTCATCCTTGTGGTGAGCGCTGAGAAGAAGCTGACAGGC
  AGTACCGTGGGCATGCGGGCCAGTGTGGAGACCAGCCCCCTGCTTCGGTTCCGGGCCGAGTCCGTGGTGC
  CCGCGCGCATGGCGGAGATGGCCCGCTGCATCCGGGAGCGAGACTTCCCCAGCTTCGCCCAGCTGACCAT
  GAAGGACAGCAACCAGTTCCACGCCACCTGCCTCGACACCTTCCCGCCCATCTCTTACCTCAATGCCATC
  TCCTGGCGCATCATCCACCTGGTGCACCGCTTCAACGCCCACCACGGGGACACCAAGGTGGCGTACACCT
  TTGACGCGGGCCCCAATGCCGTGATCTTCACCCTGGACGACACTGTGGCTGAGTTTGTGGCTGCTGTGTG
  GCACGGCTTTCCCCCAGGCTCGAATGGAGACACGTTTCTGAAGGGGCTGCAGGTGAGGCCGGCCCCTCTC
  TCAGCTGAGCTTCAGGCTGCGCTGGCCATGGAGCCGACCCCCGGTGGGGTCAAATACATCATTGTCACTC
  AGGTGGGGCCAGGGCCTCAAATCCTGGATGACCCCTGCGCCCACCTCCTGGGTCCTGACGGCCTGCCGAA
  GCCAGCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200451 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASEKPLAAVTCTAPVNIAVIKYWGKRDEELVLPINSSLSVTLHQDQLKTTTTAVISKDFTEDRIWLNGR
  EEDVGQPRLQACLREIRCLARKRRNSRDGDPLPSSLSCKVHVASVNNFPTAAGLASSAAGYACLAYTLAR
  VYGVESDLSEVARRGSGSACRSLYGGFVEWQMGEQADGKDSIARQVAPESHWPELRVLILVVSAEKKLTG
  STVGMRASVETSPLLRFRAESVVPARMAEMARCIRERDFPSFAQLTMKDSNQFHATCLDTFPPISYLNAI
  SWRIIHLVHRFNAHHGDTKVAYTFDAGPNAVIFTLDDTVAEFVAAVWHGFPPGSNGDTFLKGLQVRPAPL
  SAELQAALAMEPTPGGVKYIIVTQVGPGPQILDDPCAHLLGPDGLPKPAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002461
• ORF Size: 1200 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002461.1, NM_002461.2, NP_002452.1
• RefSeq Size: 1812 bp
• RefSeq ORF: 1203 bp
• Locus ID: 4597
• Cytogenetics: 16q24.2
• Domains: GHMP_kinases
• Protein Pathways: Metabolic pathways, Terpenoid backbone biosynthesis
• MW: 43.4 kDa
• Gene Summary: 'The enzyme mevalonate pyrophosphate decarboxylase catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate in one of the early steps in cholesterol biosynthesis. It decarboxylates and dehydrates its substrate while hydrolyzing ATP. [provided by RefSeq, Jul 2008]'