COPS3 (NM_003653) Human Tagged ORF Clone

CAT#: RC200486

COPS3 (Myc-DDK-tagged)-Human COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis) (COPS3), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY418520) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200486 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: COPS3
• Synonyms: CSN3; SGN3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200486 representing NM_003653
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCTGCCCTGGAGCAGTTCGTGAACAGTGTCCGACAGCTCTCAGCTCAAGGGCAAATGACACAGC
  TTTGTGAACTGATCAACAAGAGTGGGGAACTCCTTGCGAAGAACTTATCCCATCTGGACACTGTGCTCGG
  GGCTCTGGATGTACAAGAACACTCCTTGGGCGTCCTTGCTGTTTTGTTTGTGAAGTTTTCTATGCCCAGT
  GTTCCTGACTTCGAAACGCTATTCTCACAGGTTCAGCTCTTCATCAGCACTTGTAATGGGGAGCACATTC
  GATATGCAACAGACACTTTTGCTGGGCTTTGCCATCAGCTAACAAATGCACTTGTGGAAAGAAAACAGCC
  CCTGCGAGGAATTGGCATCCTTAAGCAAGCCATAGACAAGATGCAGATGAATACAAACCAGCTGACCTCA
  ATACATGCTGATCTCTGCCAGCTTTGTTTGCTAGCAAAATGCTTTAAGCCTGCCCTTCCATATCTTGACG
  TGGATATGATGGATATCTGTAAAGAGAATGGAGCCTATGATGCAAAACACTTTTTATGTTACTATTATTA
  TGGAGGGATGATTTATACTGGGCTGAAGAACTTTGAAAGAGCTCTCTACTTTTATGAACAGGCTATAACT
  ACTCCTGCCATGGCGGTCAGTCATATCATGTTGGAATCATATAAAAAGTATATTTTAGTGTCTTTGATAT
  TACTTGGCAAAGTACAACAGCTACCAAAATATACATCTCAAATTGTGGGTAGATTCATTAAGCCTCTTAG
  CAATGCATACCACGAGTTAGCACAAGTGTATTCAACCAACAACCCCTCAGAACTCCGAAACCTGGTGAAT
  AAGCACAGTGAAACCTTCACTCGCGATAACAACATGGGGCTGGTGAAGCAATGCTTGTCATCTCTTTATA
  AGAAGAATATTCAGAGGCTAACAAAGACCTTTTTAACTCTATCATTACAAGATATGGCAAGTCGTGTGCA
  GTTGTCTGGACCTCAGGAGGCAGAGAAATACGTTCTGCACATGATAGAAGATGGTGAGATTTTTGCAAGT
  ATTAACCAGAAGGACGGTATGGTCAGTTTCCATGATAACCCTGAAAAATATAATAACCCAGCCATGCTTC
  ATAACATTGATCAGGAGATGCTGAAGTGCATTGAGCTGGATGAGCGGCTGAAAGCCATGGACCAGGAGAT
  CACAGTGAACCCTCAGTTTGTACAAAAGAGTATGGGCTCACAAGAAGATGATTCAGGAAACAAACCATCC
  AGTTATTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200486 representing NM_003653
  Red=Cloning site Green=Tags(s)
  
  MASALEQFVNSVRQLSAQGQMTQLCELINKSGELLAKNLSHLDTVLGALDVQEHSLGVLAVLFVKFSMPS
  VPDFETLFSQVQLFISTCNGEHIRYATDTFAGLCHQLTNALVERKQPLRGIGILKQAIDKMQMNTNQLTS
  IHADLCQLCLLAKCFKPALPYLDVDMMDICKENGAYDAKHFLCYYYYGGMIYTGLKNFERALYFYEQAIT
  TPAMAVSHIMLESYKKYILVSLILLGKVQQLPKYTSQIVGRFIKPLSNAYHELAQVYSTNNPSELRNLVN
  KHSETFTRDNNMGLVKQCLSSLYKKNIQRLTKTFLTLSLQDMASRVQLSGPQEAEKYVLHMIEDGEIFAS
  INQKDGMVSFHDNPEKYNNPAMLHNIDQEMLKCIELDERLKAMDQEITVNPQFVQKSMGSQEDDSGNKPS
  SYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003653
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003653.2, NM_003653.3, NP_003644.2
• RefSeq Size: 1652 bp
• RefSeq ORF: 1272 bp
• Locus ID: 8533
• Domains: PCI
• Protein Families: Stem cell - Pluripotency
• MW: 47.7 kDa
• Gene Summary: The protein encoded by this gene possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]