Chromogranin A (CHGA) (NM_001275) Human Tagged ORF Clone

CAT#: RC200492

CHGA (Myc-DDK-tagged)-Human chromogranin A (parathyroid secretory protein 1) (CHGA)

Product Images

Western blot validation of overexpression lysate (Cat# LY400511) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200492 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CHGA protein (Cat# TP300492). The protein was produced from HEK293T cells transfected with CHGA cDNA clone (Cat# RC200492) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CHGA
• Synonyms: CGA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200492 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTCCGCCGCTGTCCTGGCTCTTCTGCTCTGCGCCGGGCAAGTCACTGCGCTCCCTGTGAACAGCC
  CTATGAATAAAGGGGATACCGAGGTGATGAAATGCATCGTTGAGGTCATCTCCGACACACTTTCCAAGCC
  CAGCCCCATGCCTGTCAGCCAGGAATGTTTTGAGACACTCCGAGGAGATGAACGGATCCTTTCCATTCTG
  AGACATCAGAATTTACTGAAGGAGCTCCAAGACCTCGCTCTCCAAGGCGCCAAGGAGAGGGCACATCAGC
  AGAAGAAACACAGCGGTTTTGAAGATGAACTCTCAGAGGTTCTTGAGAACCAGAGCAGCCAGGCCGAGCT
  GAAAGAGGCGGTGGAAGAGCCATCATCCAAGGATGTTATGGAGAAAAGAGAGGATTCCAAGGAGGCAGAG
  AAAAGTGGTGAAGCCACAGACGGAGCCAGGCCCCAGGCCCTCCCGGAGCCCATGCAGGAGTCCAAGGCTG
  AGGGGAACAATCAGGCCCCTGGGGAGGAAGAGGAGGAGGAGGAGGAGGCCACCAACACCCACCCTCCAGC
  CAGCCTCCCCAGCCAGAAATACCCAGGCCCACAGGCCGAGGGGGACAGTGAGGGCCTCTCTCAGGGTCTG
  GTGGACAGAGAGAAGGGCCTGAGTGCAGAGCCAGGGTGGCAGGCAAAGAGAGAAGAGGAGGAGGAGGAGG
  AGGAGGAGGCTGAGGCTGGAGAGGAGGCTGTCCCCGAGGAAGAAGGCCCCACTGTAGTGCTGAACCCCCA
  CCCGAGCCTTGGCTACAAGGAGATCCGGAAAGGCGAGAGTCGGTCGGAGGCTCTGGCTGTGGATGGAGCT
  GGGAAGCCTGGGGCTGAGGAGGCTCAGGACCCCGAAGGGAAGGGAGAACAGGAGCACTCCCAGCAGAAAG
  AGGAGGAGGAGGAGATGGCAGTGGTCCCGCAAGGCCTCTTCCGGGGTGGGAAGAGCGGAGAGCTGGAGCA
  GGAGGAGGAGCGGCTCTCCAAGGAGTGGGAGGACTCCAAACGCTGGAGCAAGATGGACCAGCTGGCCAAG
  GAGCTGACGGCTGAGAAGCGGCTGGAGGGGCAGGAGGAGGAGGAGGACAACCGGGACAGTTCCATGAAGC
  TCTCCTTCCGGGCCCGGGCCTACGGCTTCAGGGGCCCTGGGCCGCAGCTGCGACGAGGCTGGAGGCCATC
  CTCCCGGGAGGACAGCCTTGAGGCGGGCCTGCCCCTCCAGGTCCGAGGCTACCCCGAGGAGAAGAAAGAG
  GAGGAGGGCAGCGCAAACCGCAGACCAGAGGACCAGGAGCTGGAGAGCCTGTCGGCCATTGAGGCAGAGC
  TGGAGAAAGTGGCCCACCAGCTGCAGGCACTACGGCGGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200492 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRSAAVLALLLCAGQVTALPVNSPMNKGDTEVMKCIVEVISDTLSKPSPMPVSQECFETLRGDERILSIL
  RHQNLLKELQDLALQGAKERAHQQKKHSGFEDELSEVLENQSSQAELKEAVEEPSSKDVMEKREDSKEAE
  KSGEATDGARPQALPEPMQESKAEGNNQAPGEEEEEEEEATNTHPPASLPSQKYPGPQAEGDSEGLSQGL
  VDREKGLSAEPGWQAKREEEEEEEEEAEAGEEAVPEEEGPTVVLNPHPSLGYKEIRKGESRSEALAVDGA
  GKPGAEEAQDPEGKGEQEHSQQKEEEEEMAVVPQGLFRGGKSGELEQEEERLSKEWEDSKRWSKMDQLAK
  ELTAEKRLEGQEEEEDNRDSSMKLSFRARAYGFRGPGPQLRRGWRPSSREDSLEAGLPLQVRGYPEEKKE
  EEGSANRRPEDQELESLSAIEAELEKVAHQLQALRRG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001275
• ORF Size: 1371 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001275.1, NM_001275.2, NM_001275.3, NP_001266.1
• RefSeq Size: 2079 bp
• RefSeq ORF: 1374 bp
• Locus ID: 1113
• Cytogenetics: 14q32.12
• Domains: Granin
• Protein Families: Druggable Genome, Secreted Protein
• MW: 50.7 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]'