Chromogranin A (CHGA) (NM_001275) Human Untagged Clone

CAT#: SC119356

CHGA (untagged)-Human chromogranin A (parathyroid secretory protein 1) (CHGA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHGA
• Synonyms: CGA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119356 sequence for NM_001275 edited (data generated by NextGen Sequencing)
  ATGCGCTCCGCCGCTGTCCTGGCTCTTCTGCTCTGCGCCGGGCAAGTCACTGCGCTCCCT
  GTGAACAGCCCTATGAATAAAGGGGATACCGAGGTGATGAAATGCATCGTTGAGGTCATC
  TCCGACACACTTTCCAAGCCCAGCCCCATGCCTGTCAGCCAGGAATGTTTTGAGACACTC
  CGAGGAGATGAACGGATCCTTTCCATTCTGAGACATCAGAATTTACTGAAGGAGCTCCAA
  GACCTCGCTCTCCAAGGCGCCAAGGAGAGGGCACATCAGCAGAAGAAACACAGCGGTTTT
  GAAGATGAACTCTCAGAGGTTCTTGAGAACCAGAGCAGCCAGGCCGAGCTGAAAGAGGCG
  GTGGAAGAGCCATCATCCAAGGATGTTATGGAGAAAAGAGAGGATTCCAAGGAGGCAGAG
  AAAAGTGGTGAAGCCACAGACGGAGCCAGGCCCCAGGCCCTCCCGGAGCCCATGCAGGAG
  TCCAAGGCTGAGGGGAACAATCAGGCCCCTGGGGAGGAAGAGGAGGAGGAGGAGGAGGCC
  ACCAACACCCACCCTCCAGCCAGCCTCCCCAGCCAGAAATACCCAGGCCCACAGGCCGAG
  GGGGACAGTGAGGGCCTCTCTCAGGGTCTGGTGGACAGAGAGAAGGGCCTGAGTGCAGAG
  CCAGGGTGGCAGGCAAAGAGAGAAGAGGAGGAGGAGGAGGAGGAGGAGGCTGAGGCTGGA
  GAGGAGGCTGTCCCCGAGGAAGAAGGCCCCACTGTAGTGCTGAACCCCCACCCGAGCCTT
  GGCTACAAGGACATCCGGAAAGGCGAGAGTCGGTCGGAGGCTCTGGCTGTGGATGGAGCT
  GGGAAGCCTGGGGCTGAGGAGGCTCAGGACCCCGAAGGGAAGGGAGAACAGGAGCACTCC
  CAGCAGAAAGAGGAGGAGGAGGAGATGGCAGTGGTCCCGCAAGGCCTCTTCCGGGGTGGG
  AAGAGCGGAGAGCTGGAGCAGGAGGAGGAGCGGCTCTCCAAGGAGTGGGAGGACTCCAAA
  CGCTGGAGCAAGATGGACCAGCTGGCCAAGGAGCTGACGGCTGAGAAGCGGCTGGAGGGG
  CAGGAGGAGGAGGAGGACAACCGGGACAGTTCCATGAAGCTCTCCTTCCGGGCCCGGGCC
  TACGGCTTCAGGGGCCCTGGGCCGCAGCTGCGACGAGGCTGGAGGCCATCCTCCCGGGAG
  GACAGCCTTGAGGCGGGCCTGCCCCTCCAGGTCCGAGGCTACCCCGAGGAGAAGAAAGAG
  GAGGAGGGCAGCGCAAACCGCAGACCAGAGGACCAGGAGCTGGAGAGCCTGTCGGCCATT
  GAGGCAGAGCTGGAGAAAGTGGCCCACCAGCTGCAGGCACTACGGCGGGGCTGA
  
  Clone variation with respect to NM_001275.3
  792 g=>c;1323 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001275 unedited
  GATTTATGTATACGACTTACTATAGTGGCGGCCGCGATTCGGCACGAGGTGCAGTGCTCG
  AGCCCCGTGCAGGGGAGCTTGCGGGAGGATCGACCGACAGACGGACGCACGCCGAGGCAC
  TGCGCCCCCAGCCCCGCGCCGGTGCCACCGCAGCCCGACCCCGGCCGCCAGTCCAGCCGC
  CCCTCGCCCGGTGCCTAGGTGCCCGGCCCCACACCGCCAGCTGCTCGGCGCCCGGGTCCG
  CCATGCGCTCCGCCGCTGTCCTGGCTCTTCTGCTCTGCGCCGGGCAAGTCACTGCGCTCC
  CTGTGAACAGCCCTATGAATAAAGGGGATACCGAGGTGATGAAATGCATCGTTGAGGTCA
  TCTCCGACACACTTTCCAAGCCCAGCCCCATGCCTGTCAGCCAGGAATGTTTTGAGACAC
  TCCGAGGAGATGAACGGATCCTTTCCATTCTGAGACATCAGAATTTACTGAAGGAGCTCC
  AAGACCTCGCTCTCCAAGGCGCCAAGGAGAGGGCACATCAGCAGAAGAAACACAGCGGTT
  TTGAAGATGAACTCTCAGAGGTTCTTGAGAACCAGAGCAGCCAGGCCGAGCTGAAAGAGG
  CGGTGGAAGAGCCATCATCCAAGGATGTTATGGAGAAAAGAGAGGATTCCAAGGAGGCAG
  AGAAAAGTGGTGAAGCCACAGACGGAGCCCGGCCCAAGCCCTTCCGGAGCCCATGCAGGA
  GTCCAAGCCTGAGGGGAACAATCANGCCCCTGGGGAGGAACAAGAGTGAGAGGAGGAGGC
  CACCCACACCACCCTTCAGNCAGTCTTCCCAGCCAGATTACCCAGGCCCACAGGCCGAGA
  GGACAGGGAGGGCCNTTCTAAGGTCTGGTGGACAGAGAAAGGCCCTGATGCAAAGCCAGG
  TGGCAGCAAGAAGAAAGGGGGAGAAGAGTAGTAGGGCTCTAGCCTGAAGGAGGTGTCCCT
  AGACAAGGCCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001275 unedited
  GCGTTTCGGGAGACCCANACAGCTTTATTTTTCTTTAGNTAAAGTTCATACAGAATATGT
  TACAGTCAGGAGTTCTCAGCTTTCACCAAAGGTCAGAAGTTATTGCAGTTGTGCCCAGTG
  GATAGGATGGAGGAAGGGAAACTTCTAGAAAGCTGCCTGTGTTCAGAGAAGGAATCGTCA
  ATGTTTAAAGTTGTGGGGCTGCCCTGCAAAGGATGTTCCAGGGCAGGTAGGAGTCAGGAG
  TAGGAGACAAGGCGCGTAGGGGGCGATAGGGTGGCCTGGGCTTGGGCAGGCTGGAGGCTG
  CCTCCCTACCGGAAGCAGCGTCCGGGCCATCTGGCCAGGACCTGCCAAGGGGACAGCAGA
  GCCAGGGCCACAGGGTGCCCTGGGGCCAGCCCTGCCAGCCGGTGTCTCAGCCCCGCCGTA
  GTGCCTGCAGCTGGTGGGCCACTTTCTCCAGCTCTGCCTCAATGGCCGACAGGCTCTCCA
  GCTCCTGGTCCTCTGGTCTGCGGTTTGCGCTGCCCTCCTCCTCTTTCTTCTCCTCGGGGT
  AGCCTCGGACCTGGAGGGGCAGGCCCGCCTCAAGGCTGTCCTCCCGGGAGGATGGCCTCC
  AGCCTCGTCGCAGCTGCGGCCCAGGGCCCCTGAAGCCGTAGGCCCGGGCCCGGAAGGAGA
  GCTTCATGGAACTGTCCCGGTTGTCCTCCTCCTTCTCCTGCCCCTCCAGCCGCTTCTCAG
  CCGTCAGCTCCTTGGCCAGCTGGTCCATCTTGCTCCAGCGTTTGGAGTCCTCCCACTCCT
  TGGAGAGCCGNTCCTCCTNCTGCTCCAGCTCTCCGCTCTTTCCACCCCCGNAGAAGCCTT
  GCGGGACCACTGNCATTTNCTNCTCCTNCTCTTTCTGCTGGGAGTGCTCCTGTNCTCCCT
  TCCTTTCGGGTCTGAGCCTTCTCAGCCCAGCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001275
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001275.2, NP_001266.1
• RefSeq Size: 1994 bp
• RefSeq ORF: 1374 bp
• Locus ID: 1113
• Cytogenetics: 14q32.12
• Domains: Granin
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).