ZNF202 (NM_003455) Human Tagged ORF Clone

CAT#: RC200542

ZNF202 (Myc-DDK-tagged)-Human zinc finger protein 202 (ZNF202)


  "NM_003455" in other vectors (6)

Reconstitution Protocol

USD 98.00

USD 560.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ZNF202
Synonyms ZKSCAN10; ZSCAN42
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200542 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTACAGCCGTGGAACCAGAGGACCAGGATCTTTGGGAAGAAGAGGGAATTCTGATGGTGAAACTGG
AAGATGATTTCACCTGTCGGCCAGAGTCTGTCTTACAGAGGGATGACCCGGTGCTGGAAACCTCCCACCA
GAACTTCCGACGCTTCCGCTACCAGGAGGCAGCAAGCCCTAGAGAAGCTCTCATCAGACTCCGAGAACTT
TGTCACCAGTGGCTGAGACCAGAGAGGCGGACAAAGGAGCAGATCCTAGAGCTGCTTGTGCTGGAACAAT
TTCTTACCGTCCTACCTGGAGAACTACAGAGCTGGGTGCGGGGCCAACGGCCAGAAAGTGGCGAGGAGGC
AGTGACGCTGGTGGAGGGTTTGCAGAAACAACCCAGGAGACCAAGGCGGTGGGTGACTGTCCATGTTCAC
GGCCAGGAAGTCCTGTCAGAGGAGACGGTGCATTTAGGAGCGGAGCCTGAGTCACCTAATGAGCTGCAGG
ATCCTGTGCAAAGCTCGACCCCCGAGCAGTCTCCTGAGGAAACCACACAGAGCCCAGATCTGGGGGCACC
GGCAGAGCAGCGTCCACACCAGGAAGAGGAGCTCCAGACCCTGCAGGAGAGCGAGGTCCCAGTGCCCGAG
GACCCAGACCTTCCTGCAGAGAGGAGCTCTGGAGACTCAGAGATGGTTGCTCTTCTTACTGCTCTGTCAC
AGGGACTGGTAACGTTCAAGGATGTGGCCGTATGCTTTTCCCAGGACCAGTGGAGTGATCTGGACCCAAC
ACAGAAAGAGTTCTATGGAGAATATGTCTTGGAAGAAGACTGTGGAATTGTTGTCTCTCTGTCATTTCCA
ATCCCCAGACCTGATGAGATCTCCCAGGTTAGAGAGGAAGAGCCTTGGGTCCCAGATATCCAAGAGCCTC
AGGAGACTCAAGAGCCAGAAATCCTGAGTTTTACCTACACAGGAGATAGGAGTAAAGATGAGGAAGAGTG
TCTGGAGCAGGAAGATCTGAGTTTGGAGGATATACACAGGCCTGTTTTGGGAGAACCAGAAATTCACCAG
ACTCCAGATTGGGAAATAGTCTTTGAGGACAATCCAGGTAGACTTAATGAAAGAAGATTTGGTACTAATA
TTTCTCAAGTGAATAGTTTTGTGAACCTTCGGGAAACTACACCCGTCCACCCCCTGTTAGGGAGGCATCA
TGACTGTTCTGTGTGTGGAAAGAGCTTCACTTGTAACTCCCACCTTGTTAGACACCTGAGGACTCACACA
GGAGAGAAACCCTATAAATGTATGGAATGTGGAAAAAGTTACACACGAAGCTCACATCTTGCCAGGCACC
AAAAGGTTCACAAGATGAACGCGCCTTACAAATATCCCCTAAACCGGAAGAATTTGGAAGAGACCTCCCC
TGTGACACAGGCTGAGAGAACTCCATCAGTGGAGAAACCCTATAGATGTGATGATTGCGGAAAGCACTTC
CGCTGGACTTCAGACCTTGTCAGACATCAGAGGACACATACTGGAGAAAAACCCTTCTTTTGTACTATTT
GTGGCAAAAGCTTCAGCCAGAAATCTGTGTTAACAACACACCAAAGAATCCACCTGGGAGGCAAACCCTA
CTTGTGTGGAGAGTGTGGTGAGGACTTCAGTGAACACAGGCGGTACCTGGCGCACCGGAAGACGCACGCT
GCTGAGGAACTCTACCTCTGCAGCGAGTGCGGGCGCTGCTTCACCCACAGCGCAGCGTTCGCCAAGCACT
TGAGAGGACACGCCTCAGTGAGGCCCTGCCGATGCAACGAATGTGGGAAGAGCTTCAGTCGCAGGGACCA
CCTCGTCAGGCATCAGAGAACACACACTGGGGAGAAACCATTCACGTGCCCTACCTGTGGAAAAAGCTTC
AGCAGAGGATATCACTTAATTAGGCATCAGAGGACCCACTCAGAAAAGACCTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200542 protein sequence
Red=Cloning site Green=Tags(s)

MATAVEPEDQDLWEEEGILMVKLEDDFTCRPESVLQRDDPVLETSHQNFRRFRYQEAASPREALIRLREL
CHQWLRPERRTKEQILELLVLEQFLTVLPGELQSWVRGQRPESGEEAVTLVEGLQKQPRRPRRWVTVHVH
GQEVLSEETVHLGAEPESPNELQDPVQSSTPEQSPEETTQSPDLGAPAEQRPHQEEELQTLQESEVPVPE
DPDLPAERSSGDSEMVALLTALSQGLVTFKDVAVCFSQDQWSDLDPTQKEFYGEYVLEEDCGIVVSLSFP
IPRPDEISQVREEEPWVPDIQEPQETQEPEILSFTYTGDRSKDEEECLEQEDLSLEDIHRPVLGEPEIHQ
TPDWEIVFEDNPGRLNERRFGTNISQVNSFVNLRETTPVHPLLGRHHDCSVCGKSFTCNSHLVRHLRTHT
GEKPYKCMECGKSYTRSSHLARHQKVHKMNAPYKYPLNRKNLEETSPVTQAERTPSVEKPYRCDDCGKHF
RWTSDLVRHQRTHTGEKPFFCTICGKSFSQKSVLTTHQRIHLGGKPYLCGECGEDFSEHRRYLAHRKTHA
AEELYLCSECGRCFTHSAAFAKHLRGHASVRPCRCNECGKSFSRRDHLVRHQRTHTGEKPFTCPTCGKSF
SRGYHLIRHQRTHSEKTS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003455
ORF Size 1944 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003455.1, NM_003455.2, NM_003455.3, NP_003446.2
RefSeq Size 4445 bp
RefSeq ORF 1947 bp
Locus ID 7753
Domains KRAB, LER, zf-C2H2
Protein Families Transcription Factors
MW 74.7 kDa
Gene Summary Transcriptional repressor that binds to elements found predominantly in genes that participate in lipid metabolism. Among its targets are structural components of lipoprotein particles (apolipoproteins AIV, CIII, and E), enzymes involved in lipid processing (lipoprotein lipase, lecithin cholesteryl ester transferase), transporters involved in lipid homeostasis (ABCA1, ABCG1), and several genes involved in processes related to energy metabolism and vascular disease. [UniProtKB/Swiss-Prot Function]

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