SART1 (NM_005146) Human Tagged ORF Clone

CAT#: RC200603

SART1 (Myc-DDK-tagged)-Human squamous cell carcinoma antigen recognized by T cells (SART1)

Product Images

Western blot validation of overexpression lysate (Cat# LY417488) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200603 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SART1
• Synonyms: Ara1; HAF; HOMS1; SART1259; SNRNP110; Snu66
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200603 representing NM_005146
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTCGTCCAAGAAGCATCGCGGAGAGAAGGAGGCGGCCGGGACGACGGCGGCGGCCGGCACCGGGG
  GTGCCACCGAGCAGCCGCCGCGGCACCGGGAACACAAAAAACACAAGCACCGGAGTGGCGGCAGTGGCGG
  TAGCGGTGGCGAACGACGGAAGCGGAGCCGGGAACGTGGGGGCGAGCGCGGGAGCGGGCGGCGCGGGGCC
  GAAGCTGAGGCCCGGAGCAGCACGCACGGGCGGGAGCGCAGCCAGGCAGAGCCCTCCGAGCGGCGCGTGA
  AGCGGGAGAAGCGCGATGACGGCTACGAGGCCGCTGCCAGCTCCAAAACTAGCTCAGGCGATGCCTCCTC
  ACTCAGCATCGAGGAGACTAACAAACTCCGGGCAAAGTTGGGGCTGAAACCCTTGGAGGTTAATGCCATC
  AAGAAGGAGGCGGGCACCAAGGAGGAGCCCGTGACAGCTGATGTCATCAACCCTATGGCCTTGCGACAGC
  GAGAGGAGCTGCGGGAGAAGCTGGCGGCTGCCAAGGAGAAGCGCCTGCTGAACCAAAAGCTGGGGAAGAT
  AAAGACCCTAGGAGAGGATGACCCCTGGCTGGACGACACTGCAGCCTGGATCGAGAGGAGCCGGCAGCTG
  CAGAAGGAGAAGGACCTGGCAGAGAAGAGGGCCAAGTTACTGGAGGAGATGGACCAAGAGTTTGGTGTCA
  GCACTCTGGTGGAGGAGGAGTTCGGGCAGAGGCGGCAGGACCTGTACAGTGCCCGGGACCTGCAGGGCCT
  CACCGTGGAGCATGCCATTGATTCCTTCCGAGAAGGGGAGACAATGATTCTTACCCTCAAGGACAAAGGC
  GTGCTGCAGGAGGAGGAGGACGTGCTGGTGAACGTGAACCTGGTGGATAAGGAGCGGGCAGAGAAAAATG
  TGGAGCTGCGGAAGAAGAAGCCTGACTACCTGCCCTATGCCGAGGACGAGAGCGTGGACGACCTGGCGCA
  GCAAAAACCTCGCTCTATCCTGTCCAAGTATGACGAAGAGCTTGAAGGGGAGCGGCCACATTCCTTCCGC
  TTGGAGCAGGGCGGCACGGCTGATGGCCTGCGGGAGCGGGAGCTGGAGGAGATCCGGGCCAAGCTGCGGC
  TGCAGGCTCAGTCCCTGAGCACAGTGGGGCCCCGGCTGGCCTCCGAATACCTCACGCCTGAGGAGATGGT
  GACCTTTAAAAAGACCAAGCGGAGGGTGAAGAAAATCCGCAAGAAGGAGAAGGAGGTAGTAGTGCGGGCA
  GATGACTTGCTGCCTCTCGGGGACCAGACTCAGGATGGGGACTTTGGTTCCAGACTGCGGGGACGGGGTC
  GCCGCCGAGTGTCCGAAGTGGAGGAGGAGAAGGAGCCTGTGCCTCAGCCCCTGCCGTCGGACGACACCCG
  AGTGGAGAACATGGACATCAGTGATGAGGAGGAAGGTGGAGCTCCACCGCCGGGGTCCCCGCAGGTGCTG
  GAGGAGGACGAGGCGGAGCTGGAGCTGCAGAAGCAGCTGGAGAAGGGACGCCGGCTGCGACAGTTACAGC
  AGCTACAGCAGCTGCGAGACAGTGGCGAGAAGGTGGTGGAGATTGTGAAGAAGCTGGAGTCTCGCCAGCG
  GGGCTGGGAGGAGGATGAGGATCCCGAGCGGAAGGGGGCCATCGTGTTCAACGCCACGTCCGAGTTCTGC
  CGCACCTTGGGGGAGATCCCCACCTACGGGCTGGCTGGCAATCGCGAGGAGCAGGAGGAGCTCATGGACT
  TTGAACGGGATGAGGAGCGCTCAGCCAACGGTGGCTCCGAATCTGACGGGGAGGAGAACATCGGCTGGAG
  CACGGTGAACCTGGACGAGGAGAAGCAGCAGCAGGATTTCTCTGCTTCCTCCACCACCATCCTGGACGAG
  GAACCGATCGTGAATAGGGGGCTGGCAGCTGCCCTGCTCCTGTGTCAGAACAAAGGGCTGCTGGAGACCA
  CAGTGCAGAAGGTGGCCCGGGTGAAGGCCCCCAACAAGTCGCTGCCCTCAGCCGTGTACTGCATCGAGGA
  TAAGATGGCCATCGATGACAAGTACAGCCGGAGGGAGGAATACCGAGGCTTCACACAGGACTTCAAGGAG
  AAGGACGGCTACAAACCCGACGTTAAGATCGAATACGTGGATGAGACGGGCCGGAAACTCACACCCAAGG
  AGGCTTTCCGGCAGCTGTCGCACCGCTTCCATGGCAAGGGCTCAGGCAAGATGAAGACAGAGCGGCGGAT
  GAAGAAGCTGGACGAGGAGGCGCTCCTGAAGAAGATGAGCTCCAGCGACACGCCCCTGGGCACCGTGGCC
  CTGCTCCAGGAGAAGCAGAAGGCTCAGAAGACCCCCTACATCGTGCTCAGCGGCAGCGGCAAGAGCATGA
  ACGCGAACACCATCACCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200603 representing NM_005146
  Red=Cloning site Green=Tags(s)
  
  MGSSKKHRGEKEAAGTTAAAGTGGATEQPPRHREHKKHKHRSGGSGGSGGERRKRSRERGGERGSGRRGA
  EAEARSSTHGRERSQAEPSERRVKREKRDDGYEAAASSKTSSGDASSLSIEETNKLRAKLGLKPLEVNAI
  KKEAGTKEEPVTADVINPMALRQREELREKLAAAKEKRLLNQKLGKIKTLGEDDPWLDDTAAWIERSRQL
  QKEKDLAEKRAKLLEEMDQEFGVSTLVEEEFGQRRQDLYSARDLQGLTVEHAIDSFREGETMILTLKDKG
  VLQEEEDVLVNVNLVDKERAEKNVELRKKKPDYLPYAEDESVDDLAQQKPRSILSKYDEELEGERPHSFR
  LEQGGTADGLRERELEEIRAKLRLQAQSLSTVGPRLASEYLTPEEMVTFKKTKRRVKKIRKKEKEVVVRA
  DDLLPLGDQTQDGDFGSRLRGRGRRRVSEVEEEKEPVPQPLPSDDTRVENMDISDEEEGGAPPPGSPQVL
  EEDEAELELQKQLEKGRRLRQLQQLQQLRDSGEKVVEIVKKLESRQRGWEEDEDPERKGAIVFNATSEFC
  RTLGEIPTYGLAGNREEQEELMDFERDEERSANGGSESDGEENIGWSTVNLDEEKQQQDFSASSTTILDE
  EPIVNRGLAAALLLCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDDKYSRREEYRGFTQDFKE
  KDGYKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVA
  LLQEKQKAQKTPYIVLSGSGKSMNANTITK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005146
• ORF Size: 2400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005146.1, NM_005146.2, NM_005146.3, NM_005146.4, NP_005137.1
• RefSeq Size: 3601 bp
• RefSeq ORF: 2403 bp
• Locus ID: 9092
• Cytogenetics: 11q13.1
• Domains: SART-1
• Protein Pathways: Spliceosome
• MW: 90.7 kDa
• Gene Summary: This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway. [provided by RefSeq, Jul 2008]