MGAT1 (NM_002406) Human Tagged ORF Clone

CAT#: RC200717

MGAT1 (Myc-DDK-tagged)-Human mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (MGAT1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY426497) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225735 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MGAT1
• Synonyms: GLCNAC-TI; GLCT1; GLYT1; GNT-1; GNT-I; GnTI; MGAT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200717 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAAGAAGCAGTCTGCAGGGCTTGTGCTGTGGGGCGCTATCCTCTTTGTGGCCTGGAATGCCCTGC
  TGCTCCTCTTCTTCTGGACGCGCCCAGCACCTGGCAGGCCACCCTCAGTCAGCGCTCTCGATGGCGACCC
  CGCCAGCCTCACCCGGGAAGTGATTCGCCTGGCCCAAGACGCCGAGGTGGAGCTGGAGCGGCAGCGTGGG
  CTGCTGCAGCAGATCGGGGATGCCCTGTCGAGCCAGCGGGGGAGGGTGCCCACCGCGGCCCCTCCCGCCC
  AGCCGCGTGTGCCTGTGACCCCCGCGCCGGCGGTGATTCCCATCCTGGTCATCGCCTGTGACCGCAGCAC
  TGTTCGGCGCTGCCTGGACAAGCTGCTGCATTATCGGCCCTCGGCTGAGCTCTTCCCCATCATCGTTAGC
  CAGGACTGCGGGCACGAGGAGACGGCCCAGGCCATCGCCTCCTACGGCAGCGCGGTCACGCACATCCGGC
  AGCCCGACCTGAGCAGCATTGCGGTGCCGCCGGACCACCGCAAGTTCCAGGGCTACTACAAGATCGCGCG
  CCACTACCGCTGGGCGCTGGGCCAGGTCTTCCGGCAGTTTCGCTTCCCCGCGGCCGTGGTGGTGGAGGAT
  GACCTGGAGGTGGCCCCGGACTTCTTCGAGTACTTTCGGGCCACCTATCCGCTGCTGAAGGCCGACCCCT
  CCCTGTGGTGCGTCTCGGCCTGGAATGACAACGGCAAGGAGCAGATGGTGGACGCCAGCAGGCCTGAGCT
  GCTCTACCGCACCGACTTTTTCCCTGGCCTGGGCTGGCTGCTGTTGGCCGAGCTCTGGGCTGAGCTGGAG
  CCCAAGTGGCCAAAGGCCTTCTGGGACGACTGGATGCGGCGGCCGGAGCAGCGGCAGGGGCGGGCCTGCA
  TACGCCCTGAGATCTCAAGAACGATGACCTTTGGCCGCAAGGGTGTGAGCCACGGGCAGTTCTTTGACCA
  GCACCTCAAGTTTATCAAGCTGAACCAGCAGTTTGTGCACTTCACCCAGCTGGACCTGTCTTACCTGCAG
  CGGGAGGCCTATGACCGAGATTTCCTCGCCCGCGTCTACGGTGCTCCCCAGCTGCAGGTGGAGAAAGTGA
  GGACCAATGACCGGAAGGAGCTGGGGGAGGTGCGGGTGCAGTATACGGGCAGGGACAGCTTCAAGGCTTT
  CGCCAAGGCTCTGGGTGTCATGGATGACCTTAAGTCGGGGGTTCCGAGAGCTGGCTACCGGGGTATTGTC
  ACCTTCCAGTTCCGGGGCCGCCGTGTCCACCTGGCGCCCCCACCGACGTGGGAGGGCTATGATCCTAGCT
  GGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200717 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLKKQSAGLVLWGAILFVAWNALLLLFFWTRPAPGRPPSVSALDGDPASLTREVIRLAQDAEVELERQRG
  LLQQIGDALSSQRGRVPTAAPPAQPRVPVTPAPAVIPILVIACDRSTVRRCLDKLLHYRPSAELFPIIVS
  QDCGHEETAQAIASYGSAVTHIRQPDLSSIAVPPDHRKFQGYYKIARHYRWALGQVFRQFRFPAAVVVED
  DLEVAPDFFEYFRATYPLLKADPSLWCVSAWNDNGKEQMVDASRPELLYRTDFFPGLGWLLLAELWAELE
  PKWPKAFWDDWMRRPEQRQGRACIRPEISRTMTFGRKGVSHGQFFDQHLKFIKLNQQFVHFTQLDLSYLQ
  REAYDRDFLARVYGAPQLQVEKVRTNDRKELGEVRVQYTGRDSFKAFAKALGVMDDLKSGVPRAGYRGIV
  TFQFRGRRVHLAPPPTWEGYDPSWN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002406
• ORF Size: 1335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002406.1, NM_002406.2, NM_002406.3, NP_002397.1
• RefSeq Size: 2937 bp
• RefSeq ORF: 1338 bp
• Locus ID: 4245
• Cytogenetics: 5q35.3
• Domains: GNT-I
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• MW: 50.9 kDa
• Gene Summary: 'There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'