MGAT1 (NM_002406) Human Untagged Clone

CAT#: SC118672

MGAT1 (untagged)-Human mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (MGAT1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MGAT1
• Synonyms: GLCNAC-TI; GLCT1; GLYT1; GNT-1; GNT-I; GnTI; MGAT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118672 sequence for NM_002406 edited (data generated by NextGen Sequencing)
  ATGCTGAAGAAGCAGTCTGCAGGGCTTGTGCTGTGGGGCGCTATCCTCTTTGTGGCCTGG
  AATGCCCTGCTGCTCCTCTTCTTCTGGACGCGCCCAGCACCTGGCAGGCCACCCTCAGTC
  AGCGCTCTCGATGGCGACCCCGCCAGCCTCACCCGGGAAGTGATTCGCCTGGCCCAAGAC
  GCCGAGGTGGAGCTGGAGCGGCAGCGTGGGCTGCTGCAGCAGATCGGGGATGCCCTGTCG
  AGCCAGCGGGGGAGGGTGCCCACCGCGGCCCCTCCCGCCCAGCCGCGTGTGCCTGTGACC
  CCCGCGCCGGCGGTGATTCCCATCCTGGTCATCGCCTGTGACCGCAGCACTGTTCGGCGC
  TGCCTGGACAAGCTGCTGCATTATCGGCCCTCGGCTGAGCTCTTCCCCATCATCGTTAGC
  CAGGACTGCGGGCACGAGGAGACGGCCCAGGCCATCGCCTCCTACGGCAGCGCGGTCACG
  CACATCCGGCAGCCCGACCTGAGCAGCATTGCGGTGCCGCCGGACCACCGCAAGTTCCAG
  GGCTACTACAAGATCGCGCGCCACTACCGCTGGGCGCTGGGCCAGGTCTTCCGGCAGTTT
  CGCTTCCCCGCGGCCGTGGTGGTGGAGGATGACCTGGAGGTGGCCCCGGACTTCTTCGAG
  TACTTTCAGGCCACCTATCCGCTGCTGAAGGCCGACCCCTCCCTGTGGTGCGTCTCGGCC
  TGGAATGACAACGGCAAGGAGCAGATGGTGGACGCCAGCAGGCCTGAGCTGCTCTACCGC
  ACCGACTTTTTCCCTGGCCTGGGCTGGCTGCTGTTGGCCGAGCTCTGGGCTGAGCTGGAG
  CCCAAGTGGCCAAAGGCCTTCTGGGACGACTGGATGCGGCGGCCGGAGCAGCGGCAGGGG
  CGGGCCTGCATACGCCCTGAGATCTCAAGAACGATGACCTTTGGCCGCAAGGGTGTGAGC
  CACGGGCAGTTCTTTGACCAGCACCTCAAGTTTATCAAGCTGAACCAGCAGTTTGTGCAC
  TTCACCCAGCTGGACCTGTCTTACCTGCAGCGGGAGGCCTATGACCGAGATTTCCTCGCC
  CGCGTCTACGGTGCTCCCCAGCTGCAGGTGGAGAAAGTGAGGACCAATGACCGGAAGGAG
  CTGGGGGAGGTGCGGGTGCAGTATACGGGCAGGGACAGCTTCAAGGCTTTCGCCAAGGCT
  CTGGGTGTCATGGATGACCTTAAGTCGGGGGTTCCGAGAGCTGGCTACCGGGGTATTGTC
  ACCTTCCAGTTCCGGGGCCGCCGTGTCCACCTGGCGCCCCCACCGACGTGGGAGGGCTAT
  GATCCTAGCTGGAATTAG
  
  Clone variation with respect to NM_002406.3
  668 g=>a;1304 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002406 unedited
  NNTTTGTCAAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGTCAGC
  TCAGCCATGGACTTTGGACCTGGGTAAGAAGAGAGAAGCTGCACTTTGGTGGATCTGATT
  TGGGATTCTCAGTTTTGAAAATGGAGTGCTGAATGGGGATTTGATGATCTCCTGGAGAGC
  AACTGAGACAAGAGAAGAAAGGTGCATGGCTGCCTCCTAATCCCATAGTCCAGAGGAGGC
  ATCCCTAGGACTGCGGGCAAGGGAGCCGGGCAAGCCCAGGGCAGCCTTGAACCGTCCCCT
  GGCCTGCCCTCCCCGGTGGGGGCCAGGATGCTGAAGAAGCAGTCTGCAGGGCTTGTGCTG
  TGGGGCGCTATCCTCTTTGTGGCCTGGAATGCCCTGCTGCTCCTCTTCTTCTGGACGCGC
  CCAGCACCTGGCAGGCCACCCTCAGTCAGCGCTCTCGATGGCGACCCCGCCAGCCTCACC
  CGGGAAGTGATTCGCCTGGCCCAAGACGCCGAGGTGGAGCTGGAGCGGCAGCGTGGGCTG
  CTGCAGCAGATCGGGGATGCCCTGTCGAGCCAGCGGGGNGAGGGTGCCCACCGCGGCCCC
  TCCCGCCCAGCCGCGTGTGCCTGTGACCCCCGCGCCGGCGGTGATTCCCATCCTGGTCAT
  CGCCTGTGACCGCAGCACTGTTCGGCGCTGCCTGGACAAGCTGCTGCATTATCGGCCCTC
  GGCTGAGCTCTTCCCATCATCGTTAGCCAGGACTGCGGGCACGAGGAGACGGCCCAGGCC
  ATCGCCTTCTACGGNAGACGCGGTCACGCACATTCGGCAGCCCGACCTGAGACATTTTTG
  CGGTGCCGNCGGACCACCCGCAGTTCCAGGGCTACTACAGGATCGCGCGCCTTACGCTGG
  GGCGCTGGGCCAGCTTTTNCCGTAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002406 unedited
  GGGGGGGGANNNNNNNNCCTCTTTTNNNNNNNNNCCTCTGTGNACCTGGACCGCATTTTA
  NGATCNGTTTTTTTTTTTTTTTTTTACAAACATTCAGCCTTTTATTTTAAAAAAAATTCT
  GTAGCTTCCACTTTCTTTCATGAAACTGAGGTCAGGCAAGAAACAAAAATCCACCAAGTC
  CTCTCCATCCTGCCATGGCGTCCTGGCCTGTGAGGACATGGGGCGCCTGGGAGCGGGCGG
  GGAGGCTGGGCAGCACTGGGCCAGAGGCGTCCTGGTCACTGCTCCACCTGGTCACTGCTC
  CACCTCATGCTGAGAGGAGCCTGTGTGTCAAACCCCAGGGGAAAAAGGGACAGGCAGATC
  GAATTCTGTCTTCTACCAAGCCAGCAGGAGCCTCCTAGAGCAACAGGGAGGGCACTGATT
  TTCAGAAAGGAGGGGTCAGGGCATAGTCCCTCTCTGAGGTAAGGCAAGAGAGAGGTTGTC
  CCAGCACAAGGCCTTCCTCCCTGCACCTCTCTCCTGACAGCTGGGCATGGGCTGAGGAGA
  GGTCTTGCTTGCCCCCTTCAACTTTCCATCTCAGAACTATAAACTGCTAGGCTGCAAGGA
  GAGAGGGCTAAGTGGGGGTCAAACAGGANAGAAGGGCAGGAGGCANTGAGCCCCGATGAC
  CCACCAACTNCACCNAGCCCTNGACAGGAAGCCCCTTTGGTTAGTATCATTTTGGCCACA
  AATATGTCCCCCTTCTCCCATAACTCAGTCCCCACTGGNGGAAAGGGGAGCANGTATANA
  AGGAGGGCTCGTGCCCCTGNNCTGAAAAACCAGGTCAGGNAAAAGGGGTCTGGTCAAGAA
  AGCGAACGGTGCCAAACTCTCTGCCCATGCTCCAAGAGAAAGCTCAAGACGTGGGCAATT
  TCTGGCCCCCACAAGCNCCAGGGCCCCCCACCCAACAGTGGGTTTCCTGTCTAATACCCC
  CGCACAATACCTAAAATAAGTCCCCCTGAACTGNACTCCCTTGAGAAACGGAGAAATATN
  CCCTCTGNTATCATTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002406
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002406.2, NP_002397.1
• RefSeq Size: 2937 bp
• RefSeq ORF: 1338 bp
• Locus ID: 4245
• Cytogenetics: 5q35.3
• Domains: GNT-I
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: 'There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 6. Variants 1-23 all encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and experimental data.