S adenosylhomocysteine hydrolase (AHCY) (NM_000687) Human Tagged ORF Clone
CAT#: RC200724
AHCY (Myc-DDK-tagged)-Human adenosylhomocysteinase (AHCY), transcript variant 1
"NM_000687" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | AHCY |
Synonyms | adoHcyase; SAHH |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200724 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACAAACTGCCCTACAAAGTCGCCGACATCGGCCTGGCTGCCTGGGGACGCAAGGCCCTGGACA TTGCTGAGAACGAGATGCCGGGCCTGATGCGTATGCGGGAGCGGTACTCGGCCTCCAAGCCACTGAAGGG CGCCCGCATCGCTGGCTGCCTGCACATGACCGTGGAGACGGCCGTCCTCATTGAGACCCTCGTCACCCTG GGTGCTGAGGTGCAGTGGTCCAGCTGCAACATCTTCTCCACCCAGGACCATGCGGCGGCTGCCATTGCCA AGGCTGGCATTCCGGTGTATGCCTGGAAGGGCGAAACGGACGAGGAGTACCTGTGGTGCATTGAGCAGAC CCTGTACTTCAAGGACGGGCCCCTCAACATGATTCTGGACGACGGGGGCGACCTCACCAACCTCATCCAC ACCAAGTACCCGCAGCTTCTGCCAGGCATCCGAGGCATCTCTGAGGAGACCACGACTGGGGTCCACAACC TCTACAAGATGATGGCCAATGGGATCCTCAAGGTGCCTGCCATCAATGTCAATGACTCCGTCACCAAGAG CAAGTTTGACAACCTCTATGGCTGCCGGGAGTCCCTCATAGATGGCATCAAGCGGGCCACAGATGTGATG ATTGCCGGCAAGGTAGCGGTGGTAGCAGGCTATGGTGATGTGGGCAAGGGCTGTGCCCAGGCCCTGCGGG GTTTCGGAGCCCGCGTCATCATCACCGAGATTGACCCCATCAACGCACTGCAGGCTGCCATGGAGGGCTA TGAGGTGACCACCATGGATGAGGCCTGTCAGGAGGGCAACATCTTTGTCACCACCACAGGCTGTATTGAC ATCATCCTTGGCCGGCACTTTGAGCAGATGAAGGATGATGCCATTGTGTGTAACATTGGACACTTTGACG TGGAGATCGATGTCAAGTGGCTCAACGAGAACGCCGTGGAGAAGGTGAACATCAAGCCGCAGGTGGACCG GTATCGGTTGAAGAATGGGCGCCGCATCATCCTGCTGGCCGAGGGTCGGCTGGTCAACCTGGGTTGTGCC ATGGGCCACCCCAGCTTCGTGATGAGTAACTCCTTCACCAACCAGGTGATGGCGCAGATCGAGCTGTGGA CCCATCCAGACAAGTACCCCGTTGGGGTTCATTTCCTGCCCAAGAAGCTGGATGAGGCAGTGGCTGAAGC CCACCTGGGCAAGCTGAATGTGAAGTTGACCAAGCTAACTGAGAAGCAAGCCCAGTACCTGGGCATGTCC TGTGATGGCCCCTTCAAGCCGGATCACTACCGCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200724 protein sequence
Red=Cloning site Green=Tags(s) MSDKLPYKVADIGLAAWGRKALDIAENEMPGLMRMRERYSASKPLKGARIAGCLHMTVETAVLIETLVTL GAEVQWSSCNIFSTQDHAAAAIAKAGIPVYAWKGETDEEYLWCIEQTLYFKDGPLNMILDDGGDLTNLIH TKYPQLLPGIRGISEETTTGVHNLYKMMANGILKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVM IAGKVAVVAGYGDVGKGCAQALRGFGARVIITEIDPINALQAAMEGYEVTTMDEACQEGNIFVTTTGCID IILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDRYRLKNGRRIILLAEGRLVNLGCA MGHPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMS CDGPFKPDHYRY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000687 |
ORF Size | 1296 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000687.1, NM_000687.2, NM_000687.3, NP_000678.1 |
RefSeq Size | 2211 bp |
RefSeq ORF | 1299 bp |
Locus ID | 191 |
Cytogenetics | 20q11.22 |
Domains | AdoHcyase |
Protein Families | Druggable Genome |
Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways, Selenoamino acid metabolism |
MW | 47.7 kDa |
Gene Summary | 'S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC319344 | AHCY (untagged)-Human adenosylhomocysteinase (AHCY), transcript variant 1 |
USD 310.00 |
|
RG200724 | AHCY (GFP-tagged) - Human adenosylhomocysteinase (AHCY), transcript variant 1 |
USD 460.00 |
|
RC200724L1 | Lenti ORF clone of Human adenosylhomocysteinase (AHCY), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC200724L2 | Lenti ORF clone of Human adenosylhomocysteinase (AHCY), transcript variant 1, mGFP tagged |
USD 768.00 |
|
RC200724L3 | Lenti ORF clone of Human adenosylhomocysteinase (AHCY), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC200724L4 | Lenti ORF clone of Human adenosylhomocysteinase (AHCY), transcript variant 1, mGFP tagged |
USD 620.00 |
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